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تعیین سطح بهینه زرده تخم‌مرغ در رقیق‌کننده انجماد اسپرم بز با ارزیابی‌های برون تنی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 دانشجوی دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

به ­منظور مقایسه اثر سطوح مختلف زرده تخم‌مرغ بر انجمادپذیری اسپرم بز، آزمایشی با استفاده از چهار رأس بز در قالب یک طرح کاملاً تصادفی با شش تکرار انجام شد. نمونه‌ها دو بار در هفته از حیوانات جمع‌آوری و در رقیق‌کننده بر پایه زرده در دو سطح 15 و 20 درصد رقیق و سپس منجمد شدند. پس از یخ­گشایی، فراسنجه­های کیفی ازجمله ویژگی‌های حرکتی اسپرم با کمک CASA، یکپارچگی غشا، درصد سلول‌های اسپرم با غشای فعال، درصد سلول‌های اسپرم با شکل طبیعی، وضعیت آپپتوز، یکپارچگی آکروزوم و قطعه­قطعه‌شدن DNA  مورد ارزیابی قرار گرفتند. داده‌ها با استفاده از رویه GLM نرم‌افزارSAS  آنالیز شدند. جنبایی کل و جنبایی پیش‌رونده، در رقیق‌کننده حاوی 20 درصد زرده (به‌ترتیب 69/1±00/69 و 29/1±57/52) در برابر 15 درصد زرده (به‌ترتیب 69/1±21/68 و 29/1±35/52) تفاوت معنی‌دار نداشت. همچنین درصد اسپرم زنده، اسپرم با غشای فعال و درصد سلول‌های اسپرم طبیعی در رقیق‌کننده حاوی 20 درصد زرده (به­ترتیب 08/1±88/83، 43/2±80/72 و 85/1±31/84) و در رقیق‌کننده حاوی 15 درصد زرده (به­ترتیب 08/1±65/86، 43/2±37/72 و 85/1±25/79) اختلاف معنی­داری نداشتند. درصد اسپرم با آکروزوم سالم و نرخ قطعه‌قطعهشدن DNA بین دو گروه آزمایشی تفاوت معنی‌داری نداشت. اما در آزمون آپپتوزیس، سلول‌های اسپرم زنده  بیشتری در گروه حاوی 20 درصد (17/0±18/46) نسبت به گروه حاوی 15درصد (17/0±52/45) زرده دیده شد (05/0>P). داده­های حاضر نشان­دهنده بهبود نسبی انجمادپذیری اسپرم بز در رقیق­کننده حاوی 20 درصد زرده تخم­مرغ است، اما برای اظهارنظر دقیق‌تر در خصوص سطح مناسب زرده در رقیق‌کننده، به مطالعات باروری درون تنی نیاز است.

کلیدواژه‌ها


عنوان مقاله [English]

Determination of the egg yolk optimum level in the goat sperm freezing extender by In vitro evaluations

نویسندگان [English]

  • Mohammad Tar 1
  • Armin Towhidi 2
  • Saeed Zeinoaldini 3
  • Mahdi Zhandi 3
  • Mohammad Hosein Moazeni Zadeh 4
1 M. Sc. Student, Department of Animal science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Animal science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Ph.D. Candidate, Department of Animal science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This research aimed to evaluate two concentrations of egg yolk inclusion rate (15% and 20%) in the semen extender of goat semen cryopreserved during breeding season. Thirty ejaculates were collected from five trained Mahabadi goats and diluted in egg yolk based extenders. The semen samples were frozen, and after thawing evaluated for seminal characteristics i.e. sperm motility and morphology, membrane and acrosome integrity, viability, apoptosis status and DNA fragmentation. The data were analyzed using GLM procedure in SAS software. There were not significant differences in total and progressive motility of sperm in 20% egg yolk extender (69.00±1.69 and 52.57±1.29 respectively) and 15% egg yolk extender (68.21±1.69 and 52.35±1.29 respectively). Moreover, sperm viability, membrane integrity, and normal sperm cells percentage in extender with 20% and 15% egg yolk were evaluated to be (83.88±1.08, 72.80±2.43, and 84.31±1.85) and (86.65±1.08, 72.37±2.43, and 79.25±1.85) respectively. Also, in the terms of sperm with intact acrosome and DNA fragmentation rate, no significant differences were observed in the extenders. In the apoptosis test, more live sperms were observed in the group containing 20% egg yolk compared with the one containing 15% egg yolk. To elucidate the effect of diluents used in present study on sperm fertility, further studies should be conducted in vivo

کلیدواژه‌ها [English]

  • egg yolk
  • extender
  • Goat
  • Semen
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