تأثیر تنظیم‌کنندگان شیمیایی و بیولوژیکی ‏pH‏ بر هیستومورفومتری و هیستوپاتولوژی شکمبه، نگاری، ‏کبد و کلیه بره‌های عربی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد تغذیه دام، گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع ‏طبیعی خوزستان، ایران

2 استاد، گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ایران

3 دکتری تغذیه‌دام، هنرستان خوارزمی دزفول، ایران

4 استادیار، گروه دامپزشکی، دانشگاه آزاد اسلامی، واحد یاسوج، یاسوج، ایران

چکیده

پژوهش حاضر با هدف بررسی مقایسه‌ای اثر افزودنی‏های تنظیم‌کننده pH شکمبه بر هیستومورفومتری و هیستوپاتولوژی شکمبه و نگاری، کبد و کلیه انجام گرفت. در آزمایش حاضر از 12رأس بره‌ نر عربی ۱ ± 9 ماهه با وزن زنده‌ی 55/3 ± 95/35 کیلوگرم در مدت 35 روز استفاده شد. تیمارهای آزمایشی شامل ۱- جیره شاهد، ۲- جیره شاهد + بافر بی‏کربنات سدیم (بافر)، ۳- جیره شاهد + باکتری مگاسفرا السدنی و مخمر ساکرومایسیس سرویسیه (باکتری-مخمر) بودند. در پایان آزمایش، پس از ذبح بره‌ها برای مطالعات بافتی، از کبد، کلیه‌ها، شکمبه و نگاری نمونه برداری شد و مقاطع بافتی با ضخامت پنج میکرومتر تهیه شدند. ضخامت کل دیواره نگاری در تیمار شاهد از سایر تیمارها بیشتر بود و نسبت به تیمار دریافت‌کننده بافر معنی‏دار بود (05/0>P). ضخامت لایه عضلانی در نگاری در تیمار شاهد از سایر تیمارها بیشتر بود و این اختلاف نسبت به تیمار دریافت‌کننده بافر معنی‏دار بود (05/0>P). ارتفاع پرز و عمق پرز نگاری در تیمار دریافت‌کننده باکتری-مخمر به طور معنی‏داری از سایر تیمارها بیشتر بود (05/0>P). ضخامت بافت پوششی شکمبه در تیمارهای دریافت‌کننده بافر و باکتری–مخمر به طور معنی‏داری کمتر از تیمار شاهد بود (05/0>P). ارتفاع پرزهای شکمبه در تیمار شاهد بیشتر از تیمارهای دریافت‌کننده بافر و باکتری-مخمر بود. نفوذ سلول‏های التهابی در بافت‏های کبد، کلیه و دستگاه گوارش مشاهده شد. در مجموع، عوامل تنظیم‌کننده pH در جیره‏های با کنسانتره بالا باعث محافظت بافت‌های مورد مطالعه شدند. افزون بر این، اثر باکتری-مخمر به عنوان تنظیم‌کننده بیولوژیکی با بافر شیمیایی قابل رقابت بود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of chemical and biological regulators of pH on histomorphometry and ‎histopathology of rumen, reticulum, liver and kidney of Arabi lambs ‎

نویسندگان [English]

  • Freshteh Vafaee 1
  • Morteza Chaji 2
  • omid khorasani 3
  • Farshad Baghban 4
1 M.Sc. Student, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran
2 Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran
3 Ph.D. of Animal Nutrition, Kharazmi Industrial School of Dezful, Iran‎
4 Assistant Professor, Department of Veterinary Medicine, Azad University of Yasuj, Yasuj, Iran ‎
چکیده [English]

The aim of this study was to compare the effect of rumen pH-adjusting additives on histomorphometry and histopathology of rumen, reticulum, liver, and kidney. Twelve Arabi male lambs, 9±1 months old and initial body weight of 35.95±3.55 kg were used in a completely randomized design with three treatments and four replicates in 35 days. The experimental treatments consisted of 1- A control diet, 2- Control diet+sodium bicarbonate buffer, 3- Control diet+Megasphaera elsdenii, and Saccharomyces cerevisiae (bacteria-yeast). At the end of the experiment, the lambs were slaughtered and tissue sampls from the liver, kidney, reticulum, and rumen were collected, and the tissue sections with thickness of 5 μm were prepared.  The wall thickness of the reticulum in the control treatment was significantly higher than the buffer recipient treatment (P<0.05). Also, the thickness of muscle-layer in the reticulum in the control treatment was more than other treatments and was significantly higher compare to the buffer recipient treatment (P<0.05). Papillae height and Papillae depth of reticulum in the bacteria-yeast recipient treatment were significantly higher than other treatments (P<0.05). In addition, the thickness of rumen epithelial tissue in the buffer and bacteria-yeast recipient treatments was less than the control treatment (P<0.05). The height of rumen papillae in the control treatment was significantly higher than the buffer and bacteria-yeast treatments. Infiltration of inflammatory cells into the liver, kidney and gastrointestinal tissues was observed.  In general, pH regulators, in high-concentrate diets protected the tissues, which were studied in the present experiment. In addition, the addition of bacteria-yeast effects as a biological regulator is comparable to the chemical buffer.

کلیدواژه‌ها [English]

  • Buffer
  • Inflammatory cells
  • Megasphaera elsdenii
  • Saccharomyces cerevisiae
  • Tissue

مراجع

  1. Aschenbach, J. R., Borau, T. & Gäbel, G. (2002). Glucose uptake via SGLT-1 is stimulated by β2-adrenoceptors in the ruminal epithelium of sheep. The Journal of Nutrition, 132(6), 1254-1257.
  2. Aschenbach, J. R., Penner, G. B., Stumpff, F. & Gäbel, G. )2011(. Ruminant nutrition symposium: role of fermentation acid absorption in the regulation of ruminal pH. Journal of Animal Saience, 89(4), 1092-1107.
  3. Aschenbach, J. R., Zebeli, Q., Patra, A. K., Greco, G., Amasheh, S. & Penner, G. B. (2019). Symposium review: The importance of the ruminal epithelial barrier for a healthy and productive cow. Journal of Dairy Science, 102(2), 1866-1882.(1)
  4. Azimpour, S., Shaghayegh, A. & Gholam Hosseini, B. (2014). The effect of hypervitaminosis A on the liver of newborn lambs. Journal of Veterinary Clinical Research, 5(2), 1-8. ‏(In Farsi)
  5. Calsamiglia, S., Blanch, M., Ferret, A. & Moya, D. (2012). Is subacute ruminal acidosis a pH related problem? causes and tools for its control. Animal Feed Science and Technology, 172(1-2), 42–50.
  6. DeClerck, C., Wade, Z. E., Reeves, N. R., Miller, M. F., Johnson, B. J., Ducharme, G. A. & Rathmann, R. J. (2020) Influence of Megasphaera elsdenii and feeding strategies on feedlot performance, compositional growth, and carcass parameters of early weaned, beef calves. Translational Animal Science,4(2), 863-875.
  7. EL-Khodery,, EL-Boshy, M., Gaafar, K. & Elmashad, A. (2008). Hypocalcaemia in Ossimi sheep associated with feeding on beet tops (Beta vulgaris). Turkish Journal Veterinary and Animal Science, 32(3), 199-205.
  8. Erdman, A., Botts, R. L., Hemken, R. W. & Bull, L. S. (1980). Effect of dietary sodium bicarbonate and magnesium oxide on production and physiology in early lactation. Journal of Dairy Science,63(6), 923-930.
  9. Garcia Diaz,, Ferriani Branco, A., Jacovaci, F. A., Cabreira Jobim, C., Bolson, D. C. & Pratti Daniel, J. L. (2018). Inclusion of live yeast and mannan-oligosaccharides in high grain-based diets for sheep: Ruminal parameters, inflammatory response and rumen morphology. PloS One,13(2), e0193313.
  10. Hernández, J., Benedito, J. L., Abuelo, A. & Castillo, C. (2014). Ruminal acidosis in feedlot: from aetiology to prevention. The Scientific World Journal, 2014.
  11. Hovda, K. E., Guo, C., Austin, & Mcmartin K. E. (2010). Renal toxicity of ethylene glycol results from internalization of calcium oxalate crystals by proximal tubule cells. Toxicology letters, 192(3), 365-372.
  12. Joz-Ghasemi, S., Amanlou, H., Dehghan Banadaki, M., & Nagaraja, T. G. (2018). Investigation of the occurrence of liver abscesses in lactating and dry Holstein cows and its effect on some blood parameters and histology of hepatocytes. Iranian Journal of Animal Science, 48(4), 473-481.‏(In Farsi)
  13. Khan, A., Bach, A., Weary, D. M. & Von Keyserlingk, M .A. G. (2016). Invited review: Transitioning from milk to solid feed in dairy heifers. Journal of Dairy Science,99(2), 885-902.
  14. Khorasani,, Chaji, M. & Baghban, F. (2021). Effect of chemical buffer and Megasphaera elsdenii-yeast on histomorphometry and histopathology of rumen and liver of Arabian fattening lambs fed with concentrated diets. Animal Production, 23(1), 47-59.(In Farsi)
  15. Khorasani, O., Chaji, M., & Baghban, F. (2020). Comparison of the effect of sodium bicarbonate buffer with Megasphaera elsdenii as a rumen-consuming acid on growth performance, digestibility, rumen and blood parameters of lambs in high concentrate. Journal of Animal Science Researches, 30(2), 85-99.‏(In Farsi)
  16. Krause, M. & Oetzel, G. R. (2006). Understanding and preventing subacute ruminal acidosis in dairy herds: A review. Animal feed science and technology,126(3-4), 215-236.
  17. Li, G. H., Ling, B. M., Qu, M. R., You, J. M. & Song, X. Z. )2011(. Effects of several oligosaccharides on ruminal fermentation in sheep: an in vitro experiment. Revue de Medecine Veterinaire, 162(4), 192-197.
  18. Malekkhahi,, Tahmasbi, A. M., Naserian, A. A., Danesh-Mesgaran, M., Kleen, J. L., AlZahal, O. & Ghaffari, M. H. (2016). Effects of supplementation of active dried yeast and malate during sub-acute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites and milk production in dairy cows. Animal Feed Science and Technology, 213, 29-43.
  19. Mashayekhi, R., Erfani-majd, N., Sari, M. & Rezaei, M. (2020). Investigating the effects of slow-release urea and molasses on histomorphometric tissue of rumen and abomasum and rumen fermentation parameters of fattening lamb. Iranian Veterinary Journal, 16(1), 82-93.(In Farsi)
  20. Meissner,, Hagen, F., Deiner, C., Günzel, D., Greco, G., Shen, Z. & Aschenbach, J. R. (2017). Key role of short-chain fatty acids in epithelial barrier failure during ruminal acidosis. Journal of Dairy Science, 100(8), 6662-6675.
  21. Montagne,, Pluske, J. R. & Hampson, D. J. (2003). A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young nonruminant animals. Animal Feed Science and Technology, 108(1-4), 95–117.
  22. Newbold, J., Wallace, R. J., Chen, X. B. & Mcintosh, F. M. (1995). Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in vitro and in sheep. Journal of Animal Science, 73(6), 1811–1818.
  23. Niwińska,, Hanczakowska, E., Arciszewski, M. B. & Klebaniuk, R. (2017). Exogenous butyrate: implications for the functional development of ruminal epithelium and calf performance. Journals Animal, 11(9), 1522-1530.
  24. (2007). Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids and New World Camelids. National Academy Press Washington DC.
  25. Odongo, E., AlZahal, O., Lindinger, M. I., Duffield, T. F., Valdes, E. V., Terrell, S. P. & McBride, B. W. (2006). Effects of mild heat stress and grain challenge on acid-base balance and rumen tissue histology in lambs. Journal of Animal Science,84(2), 447-455.
  26. Pinloche,, McEwan, N., Marden, J. P., Bayourthe, C., Auclair, E. & Newbold, C. J. (2013). The effects of a probiotic yeast on the bacterial diversity and population structure in the rumen of cattle. PloS one, 8(7), e67824.
  27. Rose, B. D. (1989). Clinical physiology of acid-base and electrolyte disorders (3 rd ed.). Mc Grow Hill Inc. Singapore, 261-68, 478- 501.
  28. Russell, E. & Roussel, A. J. (2007). Evaluation of the ruminant serum chemistry profile. Veterinary Clinics of North America: Food Animal Practice, 23(3), 403-426.
  29. Sedighi, & Alipour, D. (2019). Assessment of probiotic effects of isolated Megasphaera elsdenii strains in Mehraban sheep and Holstein lactating cows. Animal Feed Science and Technology,248, 126-131.
  30. Shen,, Seyfert, H. M., Löhrke, B., Schneider, F., Zitnan, R., Chudy, A., Kuhla, S., Hammon, HM., Blum, JW., Martens, H. & Hagemeister, H. (2004). An energy-rich diet causes rumen papillae proliferation associated with more IGF type 1 receptors and increased plasma IGF-1 concentrations in young goats. The Journal of Nutrition, 134(1), 11-17.
  31. Silberberg,, Chaucheyras-Durand, F., Mialon, M. M., Monteils, V., Mosoni, P., Morgavi, D. P. & Martin, C. (2013). Repeated acidosis challenges and live yeast supplementation shape rumen microbiota and fermentations and modulate inflammatory status in sheep. Animal, an International Journal of Animal Bioscience,7(12), 19-10.
  32. Wang, H., Xu, M., Wang, F. N., Yu, Z. P., Yao, J. H., Zan, L. S. & Yang, F. X. (2009). Effect of dietary starch on rumen and small intestine morphology and digesta pH in goats. Livestock Science,122(1), 48-52.
  33. Zitnan, R., Kuhla, S., Nurnberg, K., Schonhusen, U., Ceresnakova, Z., Sommer, A., Baran, M., Greserova, G. & Voigt, J. (2003). Influence of the diet on the morphology of ruminal and intestinal mucosa and on intestinal carbohydrase levels in cattle. Veterinarni Medicina-Praha, 48(7), 177-182
علوم دامی ایران
دوره 52، شماره 4
بهمن 1400
صفحه 231-241

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سابقه مقاله

  • تاریخ دریافت: 21 مرداد 1400
  • تاریخ بازنگری: 27 شهریور 1400
  • تاریخ پذیرش: 11 مهر 1400

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