نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد تغذیه دام، گروه علوم دامی، دانشکده علوم دامی و صنایع غذایی، دانشگاه علوم کشاورزی و منابع ‏طبیعی خوزستان، ایران

2 استاد، گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ایران

3 دکتری تغذیه‌دام، هنرستان خوارزمی دزفول، ایران

4 استادیار، گروه دامپزشکی، دانشگاه آزاد اسلامی، واحد یاسوج، یاسوج، ایران

چکیده

پژوهش حاضر با هدف بررسی مقایسه‌ای اثر افزودنی‏های تنظیم‌کننده pH شکمبه بر هیستومورفومتری و هیستوپاتولوژی شکمبه و نگاری، کبد و کلیه انجام گرفت. در آزمایش حاضر از 12رأس بره‌ نر عربی ۱ ± 9 ماهه با وزن زنده‌ی 55/3 ± 95/35 کیلوگرم در مدت 35 روز استفاده شد. تیمارهای آزمایشی شامل ۱- جیره شاهد، ۲- جیره شاهد + بافر بی‏کربنات سدیم (بافر)، ۳- جیره شاهد + باکتری مگاسفرا السدنی و مخمر ساکرومایسیس سرویسیه (باکتری-مخمر) بودند. در پایان آزمایش، پس از ذبح بره‌ها برای مطالعات بافتی، از کبد، کلیه‌ها، شکمبه و نگاری نمونه برداری شد و مقاطع بافتی با ضخامت پنج میکرومتر تهیه شدند. ضخامت کل دیواره نگاری در تیمار شاهد از سایر تیمارها بیشتر بود و نسبت به تیمار دریافت‌کننده بافر معنی‏دار بود (05/0>P). ضخامت لایه عضلانی در نگاری در تیمار شاهد از سایر تیمارها بیشتر بود و این اختلاف نسبت به تیمار دریافت‌کننده بافر معنی‏دار بود (05/0>P). ارتفاع پرز و عمق پرز نگاری در تیمار دریافت‌کننده باکتری-مخمر به طور معنی‏داری از سایر تیمارها بیشتر بود (05/0>P). ضخامت بافت پوششی شکمبه در تیمارهای دریافت‌کننده بافر و باکتری–مخمر به طور معنی‏داری کمتر از تیمار شاهد بود (05/0>P). ارتفاع پرزهای شکمبه در تیمار شاهد بیشتر از تیمارهای دریافت‌کننده بافر و باکتری-مخمر بود. نفوذ سلول‏های التهابی در بافت‏های کبد، کلیه و دستگاه گوارش مشاهده شد. در مجموع، عوامل تنظیم‌کننده pH در جیره‏های با کنسانتره بالا باعث محافظت بافت‌های مورد مطالعه شدند. افزون بر این، اثر باکتری-مخمر به عنوان تنظیم‌کننده بیولوژیکی با بافر شیمیایی قابل رقابت بود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of chemical and biological regulators of pH on histomorphometry and ‎histopathology of rumen, reticulum, liver and kidney of Arabi lambs ‎

نویسندگان [English]

  • Freshteh Vafaee 1
  • Morteza Chaji 2
  • omid khorasani 3
  • Farshad Baghban 4

1 M.Sc. Student, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran

2 Professor, Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural ‎Resources, University of Khuzestan, Mollasani, Ahvaz, Iran

3 Ph.D. of Animal Nutrition, Kharazmi Industrial School of Dezful, Iran‎

4 Assistant Professor, Department of Veterinary Medicine, Azad University of Yasuj, Yasuj, Iran ‎

چکیده [English]

The aim of this study was to compare the effect of rumen pH-adjusting additives on histomorphometry and histopathology of rumen, reticulum, liver, and kidney. Twelve Arabi male lambs, 9±1 months old and initial body weight of 35.95±3.55 kg were used in a completely randomized design with three treatments and four replicates in 35 days. The experimental treatments consisted of 1- A control diet, 2- Control diet+sodium bicarbonate buffer, 3- Control diet+Megasphaera elsdenii, and Saccharomyces cerevisiae (bacteria-yeast). At the end of the experiment, the lambs were slaughtered and tissue sampls from the liver, kidney, reticulum, and rumen were collected, and the tissue sections with thickness of 5 μm were prepared.  The wall thickness of the reticulum in the control treatment was significantly higher than the buffer recipient treatment (P<0.05). Also, the thickness of muscle-layer in the reticulum in the control treatment was more than other treatments and was significantly higher compare to the buffer recipient treatment (P<0.05). Papillae height and Papillae depth of reticulum in the bacteria-yeast recipient treatment were significantly higher than other treatments (P<0.05). In addition, the thickness of rumen epithelial tissue in the buffer and bacteria-yeast recipient treatments was less than the control treatment (P<0.05). The height of rumen papillae in the control treatment was significantly higher than the buffer and bacteria-yeast treatments. Infiltration of inflammatory cells into the liver, kidney and gastrointestinal tissues was observed.  In general, pH regulators, in high-concentrate diets protected the tissues, which were studied in the present experiment. In addition, the addition of bacteria-yeast effects as a biological regulator is comparable to the chemical buffer.

کلیدواژه‌ها [English]

  • Buffer
  • inflammatory cells
  • Megasphaera elsdenii
  • Saccharomyces cerevisiae
  • Tissue
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