بررسی آثار تغذیه‌ای لسیتین سویا و ویتامین ‏E‏ بر شاخص‌های کیفی اسپرم در خروس‌های اجداد ‏هوبارد

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار، گروه علوم دامی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار، گروه علوم دام و طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

با به‌کارگیری 36 قطعه خروس اجداد هوبارد در سن 45 هفتگی، آزمایشی با هدف بررسی اثر ویتامین E و لسیتین سویا در خوراک، بر برخی فراسنجه­های کیفی و کمی اسپرم طراحی شد. آزمایش در طی 60 روز و با چیدمان فاکتوریل (2×2) در قالب طرح کاملا تصادفی با دو سطح لسیتین (صفر و یک درصد در هر کیلوگرم خوراک) و دو سطح ویتامین E (صفر و 300 میلی­گرم در کیلوگرم خوراک) انجام گرفت. اسپرم‌گیری در روزهای صفر، 20، 40 و 60 آزمایش انجام شد. نتایج نشان داد بالاترین غلظت و بیشترین جنبایی کل در اسپرم خروس­هایی مشاهده شد که جیره حاوی ویتامین E + لسیتین سویا دریافت کرده بودند (05/0P<). حجم اسپرم و صفات وابسته به جنبایی نیز تحت تأثیر تیمارهای آزمایشی قرار نگرفت. همچنین با اینکه تیمارهای آزمایشی اثری بر درصد مورفولوژی غیرنرمال، و درصد اسپرم با DNA آسیب­دیده نداشت، اما یکپارچگی غشای پلاسمایی، و زنده­مانی اسپرم تحت تأثیر تیمار حاوی لسیتین سویا + ویتامین E افزایش معنی­داری یافت. کمترین میزان مالون­دی­آلدئید در منی خروس­هایی یافت شد که همزمان لسیتین سویا و ویتامین E دریافت کرده بودند (05/0P<). بررسی اثر متقابل زمان× جیره در خصوص صفات جنبایی کل و پیش­رونده، غلظت اسپرم، زنده­مانی، یکپارچگی غشا و میزان مالون­دی­آلدئید نشان داد که این صفات در روزهای پایانی آزمایش، متأثر از تیمار حاوی ویتامین E + لسیتین سویا بهبود یافتند (05/0P<). بنظر می­رسد به‌کارگیری همزمان ویتامین E و لسیتین سویا در جیره خروس­های مسن، منجر به بهبود وضعیت کیفی اسپرم شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of dietary effects of soybean lecithin and vitamin E on sperm quality ‎parameters in ‎Hubbard grandparent roosters

نویسندگان [English]

  • Shahram Shabani 1
  • Morteza Mehri 2
  • Fatemeh Shirmohammad 2
  • Mohsen Sharafi 3
1 Ph.D. Candidate, Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, ‎Iran
2 Assistant Professor, Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Department of Animal and Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
چکیده [English]

Using 36 Hubbard grandparent roosters at 45 weeks of age, the present study investigated the effects of supplementing vitamin E and soybean lecithin in the roosters' diet on their semen quality. The experiment was conducted in a completely randomized design with four treatments including basal diet, basal diet supplemented with vitamin E (300 mg/kg), basal diet supplemented with lecithin (1%), and basal diet supplemented with lecithin & vitamin E. Roosters were fed their diets for 60 days and semen collection was performed on days 0, 20, 40 and 60 of the experiment. The results showed that the treatment containing vitamin E + lecithin significantly improved total sperm motility and sperm concentration (P<0.05). The semen volume and sperm kinematic values were not affected by experimental diets (P>0.05). Although experimental treatments had no effect on abnormal morphology of sperm and DNA fragmentation, but membrane integrity and sperm viability were significantly increased in roosters fed diet supplemented with soybean lecithin and vitamin E (P<0.05). Malondialdehyde (MDA) concentration was also shown to be significantly lower in treatment containing vitamin E and soybean lecithin (P<0.05). The interaction effects of time× diet on total and progressive motility, sperm concentration, viability, membrane integrity and MDA content were significant (P<0.05) and these traits improved in the last days of the experiment due to diet supplemented with vitamin E+ soybean lecithin. Overall, it was concluded that supplementing aged roosters' diet with vitamin E and lecithin appears to improve sperm quality traits.

کلیدواژه‌ها [English]

  • Rooster
  • soybean lecithin
  • sperm
  • vitamin E

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علوم دامی ایران
دوره 52، شماره 3
آذر 1400
صفحه 177-188

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سابقه مقاله

  • تاریخ دریافت: 08 خرداد 1400
  • تاریخ بازنگری: 11 تیر 1400
  • تاریخ پذیرش: 15 تیر 1400

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