بررسی آثار تغذیه‌ای لسیتین سویا و ویتامین ‏E‏ بر شاخص‌های کیفی اسپرم در خروس‌های اجداد ‏هوبارد

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار، گروه علوم دامی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار، گروه علوم دام و طیور، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران

چکیده

با به‌کارگیری 36 قطعه خروس اجداد هوبارد در سن 45 هفتگی، آزمایشی با هدف بررسی اثر ویتامین E و لسیتین سویا در خوراک، بر برخی فراسنجه­های کیفی و کمی اسپرم طراحی شد. آزمایش در طی 60 روز و با چیدمان فاکتوریل (2×2) در قالب طرح کاملا تصادفی با دو سطح لسیتین (صفر و یک درصد در هر کیلوگرم خوراک) و دو سطح ویتامین E (صفر و 300 میلی­گرم در کیلوگرم خوراک) انجام گرفت. اسپرم‌گیری در روزهای صفر، 20، 40 و 60 آزمایش انجام شد. نتایج نشان داد بالاترین غلظت و بیشترین جنبایی کل در اسپرم خروس­هایی مشاهده شد که جیره حاوی ویتامین E + لسیتین سویا دریافت کرده بودند (05/0P<). حجم اسپرم و صفات وابسته به جنبایی نیز تحت تأثیر تیمارهای آزمایشی قرار نگرفت. همچنین با اینکه تیمارهای آزمایشی اثری بر درصد مورفولوژی غیرنرمال، و درصد اسپرم با DNA آسیب­دیده نداشت، اما یکپارچگی غشای پلاسمایی، و زنده­مانی اسپرم تحت تأثیر تیمار حاوی لسیتین سویا + ویتامین E افزایش معنی­داری یافت. کمترین میزان مالون­دی­آلدئید در منی خروس­هایی یافت شد که همزمان لسیتین سویا و ویتامین E دریافت کرده بودند (05/0P<). بررسی اثر متقابل زمان× جیره در خصوص صفات جنبایی کل و پیش­رونده، غلظت اسپرم، زنده­مانی، یکپارچگی غشا و میزان مالون­دی­آلدئید نشان داد که این صفات در روزهای پایانی آزمایش، متأثر از تیمار حاوی ویتامین E + لسیتین سویا بهبود یافتند (05/0P<). بنظر می­رسد به‌کارگیری همزمان ویتامین E و لسیتین سویا در جیره خروس­های مسن، منجر به بهبود وضعیت کیفی اسپرم شود.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of dietary effects of soybean lecithin and vitamin E on sperm quality ‎parameters in ‎Hubbard grandparent roosters

نویسندگان [English]

  • Shahram Shabani 1
  • Morteza Mehri 2
  • Fatemeh Shirmohammad 2
  • Mohsen Sharafi 3
1 Ph.D. Candidate, Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, ‎Iran
2 Assistant Professor, Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Department of Animal and Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
چکیده [English]

Using 36 Hubbard grandparent roosters at 45 weeks of age, the present study investigated the effects of supplementing vitamin E and soybean lecithin in the roosters' diet on their semen quality. The experiment was conducted in a completely randomized design with four treatments including basal diet, basal diet supplemented with vitamin E (300 mg/kg), basal diet supplemented with lecithin (1%), and basal diet supplemented with lecithin & vitamin E. Roosters were fed their diets for 60 days and semen collection was performed on days 0, 20, 40 and 60 of the experiment. The results showed that the treatment containing vitamin E + lecithin significantly improved total sperm motility and sperm concentration (P<0.05). The semen volume and sperm kinematic values were not affected by experimental diets (P>0.05). Although experimental treatments had no effect on abnormal morphology of sperm and DNA fragmentation, but membrane integrity and sperm viability were significantly increased in roosters fed diet supplemented with soybean lecithin and vitamin E (P<0.05). Malondialdehyde (MDA) concentration was also shown to be significantly lower in treatment containing vitamin E and soybean lecithin (P<0.05). The interaction effects of time× diet on total and progressive motility, sperm concentration, viability, membrane integrity and MDA content were significant (P<0.05) and these traits improved in the last days of the experiment due to diet supplemented with vitamin E+ soybean lecithin. Overall, it was concluded that supplementing aged roosters' diet with vitamin E and lecithin appears to improve sperm quality traits.

کلیدواژه‌ها [English]

  • Rooster
  • soybean lecithin
  • Sperm
  • vitamin E
  1. Adabi, S., Cooper, R., Kamali, M. & Hajbabaei, A. (2011). The influence of inclusions of vitamin E and corn oil on semen traits of Japanese quail (Coturnix coturnix japonica). Animal Reproduction Science, 123(1-2), 119-125.
  2. Aitken, R., Smith, T., Lord, T., Kuczera, L., Koppers, A., Naumovski, N., Connaughton, H., Baker, M. & De Iuliis, G. (2013). On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid. Andrology, 1(2), 192-205.
  3. Akhter, S., Ansari, M.S., Rakha, B.A., Ullah, N., Andrabi, S.M. & Khalid. M. (2011). In vitro evaluation of liquid stored buffalo semen at 5c diluted in soya lecithin-based extender (Bioxcell), tris-citric egg yolk, skim milk and egg yolk-citrate extenders. Journal of Reproduction Domestic Animal, 46(1), 45-49.
  4. Attia, Y.A., Hussein, A.S., TagEl-Din, A.E., Qota, E.M., Abed El-Ghany, A.I. & El-Sudany, A.M. (2009). Improving productive and reproductive performance of dualpurpose crossbred hens in the tropics by lecithin supplementation. Tropical Animal Health Production, 41, 461-475.
  5. Attia, Y.A. & Kamel, K.I. (2012). Semen quality, testosterone, seminal plasma biochemical and antioxidant profiles of rabbit bucks fed diets supplemented with different concentrations of soybean lecithin. Animal, 6(5), 824-833.
  6. Avendano, C., Franchi, A., Taylor, S., Morshedi, M., Bocca, S. & Oehninger, S. (2009). Fragmentation of DNA in morphologically normal human spermatozoa. Fertility & Sterility, 91(4), 1077-84.
  7. Aydilek, N., Aksakal, M. & Karakilcik, A.Z. (2004). Effects of testosterone and vitamin E on the antioxidant system in rabbit testis. Andrologia, 36, 277-281.
  8. Ayinde, O.C., Ogunnowo, S. & Ogedegbe, R.A. (2012). Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats. BMC Pharmacology and Toxicology, 13(2), 1-8.
  9. Bansal, A.K. & Bilaspuri, G.S. (2011). Impacts of oxidative strees and antioxidants on semen functions. Veterinary Medicine International, 686137, 1-7.
  10. Behnamifar, A., Rahimi, S.H., Karimi Torshizi, M.A., Sharafi, M. & Grimes, J.L. (2020). Effects of dietary alpha-lipoic acid supplementation on the seminal parameters and fertility potential in aging broiler breeder roosters, Poultry Science, 100(2), 1221-1238.
  11. Burrows, W.H. & Quinn, J. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16, 19–24.
  12. Castellini, C., Lattaioli, P., Dal Bosco, A., Minelli, A. & Mugnai, C. (2003). Oxidative status and semen characteristics of rabbit buck as affected by dietary vitamin E, C and n-3 fatty acids. Reproduction Nutrition Development, 43(1), 91-103.
  13. Cerolini, S., Zaniboni, L., Maldjian, A. & Gliozzi, T. (2006). Effect of docosahexaenoic acid and a-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66, 877-886.
  14. Cerolini, S., Kelso, K.A., Noble, R.C., Speake, B.K., Pizzi, F. & Cavalchini, L.G. (1997). Relationship between spermatozoan lipid composition and fertility during aging of chickens. Biology of Reproduction, 57(5), 976-980.
  15. Chohan, K.R., Griffin, J.T. & Carrell, D.T. (2004). Evaluation of chromatin integrity in human sperm using acridine orange staining with different fixatives and after cryopreservation. Andrologia, 36(11), 321-326.
  16. Colares, J.R., Schemitt, E.G., Hartmann, R.M., Moura, R.M., Morgan-Martins, M.I., Fillmann, H.S., Fillmann, L. & Marroni, N.P. (2016). Effect of lecithin on oxidative stress in an experimental model of rats colitis induced by acetic acid. Journal of Coloproctology, 36(2), 97-103.
  17. Comhaire, F.H., Christophe, A.B., Zalata, A.A., Dhooge, W.S., Mahmoud, A.M. & Depuydt, C. (2000). The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men. Prostaglandins Leukot Essent Fatty Acids, 63(3), 159-165.
  18. Crespilho, A. M., SaFilho, M. F., Dell’Aqua, J. A., Nichi, M., Monteiro, G. A., Avanzi, B. R., Martins, A. & Papa, F.O. (2012). Comparison of in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or new lecithin based extenders. Livestock Science, 149(1), 1-6.
  19. Darestani, A., karimi, K., Zand, K. & Khodaei motlagh, M. (2019). Effect of vitamins C and E on some sperm characteristics in Varamini rooster in cold conditon. Research on Animal Production, 10(24), 66-75.
  20. Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K., Sane Ketaki, S., Ghaskadbi Saroj, S. & Lele, R.D. (2004). Free radicals and antioxidants in human health: Current status and future prospects. Journal of Association of Physicians of India, 52, 794-804.
  21. EI-Shenawy, N.S., AL-Harbi, M.S. & Hamza, R.Z. (2014).Effect of vitamin E and selenium separately and in combination onbiochemical, immunological and histological changes induced bysodium azide in male mice. Experimental and Toxicologic Pathology, 67, 65-76.
  22. Emamverdi, M., Zhandi, M., Zare Shahneh, A., Sharafi, M. & Akbari-Sharif, A. (2013). Optimization of ram semen cryopreservation using a chemically defined soybean lecithin-based Reproduction Domestic Animal, 48(1), 899-904.
  23. Ernster, L. (1993). Lipid peroxidation in biological membranes: mechanisms and implications. In: K. Yagi (Ed), Active oxygen, lipid peroxides and antioxidants. (pp. 1-38.) CRC Press, Boca Raton.
  24. Esterbauer, H., Schaur, R.J. & Zollner, H. (1991). Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology and Medicine, 11, 81-128.
  25. Fouad, A.M., Senousey, H.K., Ruan, D., Xia, W., Chen, W., Wang, S. & Zheng, C. (2020). Nutritional modulation of fertility in male poultry. Poultry Science, 99 (11), 5637-5646.
  26. Fouad, A.M., Ruan, D., El-Senousey, H.K., Chen, W., Jiang, S. & Zheng, C. (2019). Harmful effects and control strategies of aflatoxin B1 produced by Aspergillus flavus and Aspergillus parasiticus strains on poultry: Review. Toxins, 11(3), 176.
  27. Fulbert, J.C. & Cals, M.J. (1992). Free radicals in clinical biology. Origin, pathogenic effect and defense mechanisms. Pathologie Biologie, 40, 66–77.
  28. Gallardo, M., Pérez, D., Strobel, P., Cárcamo, J. & Leighton, F. (2015). Cholesterol and vitamin E determination in broiler chickens fed canola oil. Archivos de Medicina Veterinaria, 47(2), 221-224.
  29. Gliozzi, T.M., Zaniboni, L., Maldjian, A., Luzi, F., Maertens, L. & Cerolini, S. (2009). Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets. Theriogenology, 71(6), 910-919.
  30. Herrara, C., Brogliatti, G., Cavia, R., Conde, P., Revora, M. & Pasqualini, R. S. (2004). CASA sperm parameters and their relation with in vitro fertilization. In: Proceedings of the 15th International Congress on Animal Reproduction, 8-12 Aug., Porto Seguro, Brazil, pp. 411-415.
  31. Hocking, P. & Bernard, R. (2000). Effects of the age of male and female broiler breeders on sexual behaviour, fertility and hatchability of eggs. British Poultry Science, 41, 370-376.
  32. Jaldin, R.G., Falcão, H.A., Sequeira, J.L. & Yoshida, W.B. (2006). O processo aterosclerótico em artérias de coelhos submetidos à dieta suplementada com gema de ovo: modelo experimental de baixo custo. Jornal Vascular Brasileiro, 5, 247-256.
  33. Judde, A., Villeneuve, P., Rossignol-Castera, A. & Le Guillou, A. (2003). Antioxidant effect of soy lecithins on vegetable oil stability and their synergism with tocopherols. Journal of the American Oil Chemists' Society, 80, 1209-1215.
  34. Khan, R.U., Rahman, Z.U., Javed, I. & Muhammad, F. (2012). Effect of vitamins, probiotics and protein on semen traits in post-molt male broiler breeders. Animal Reproduction Science, 135(1–4), 85-90.
  35. Long, J.A., Bongalhardo, D.C., Pelaez, J., Saxena, S., Settar, P., O’Sullivan, N.P. & Fulton, J.E. (2010). Rooster semen cryopreservation: effect of pedigree line and male age on postthaw sperm function. Poultry Science, 89, 966-973.
  36. Lotfi, S., Mehri, M., Sharafi, M. & Masoudi, R. (2017). Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster. Animal Reproduction Science, 184, 204–210.
  37. Min, Y., Sun, T., Niu, Z. & Liu, F. (2016). Vitamin C and vitamin E supplementation alleviates oxidative stress induced by dexamethasone and improves fertility of breeder roosters. Animal Reproduction Science, 171, 1-6.
  38. Mohamad Asrol, K. & Abdul Rashid, B. (2017). Effect of vitamin E supplementation on semen quantity and quality of Local Kampong roosters. Malaysian Journal of Animal Science, 20(1), 37-43.
  39. Najafi, A., Taheri, R.A., Mehdipour, M., Martínez-Pastor, F., Rouhollahi, A. & Nourian, M.R. (2019). Improvement of post-thawed sperm quality in broiler breeder roosters by ellagic acid-loaded liposomes. Poultry Science, 98(1), 440–446.
  40. Papa, F.O., Felicio, G.B., Melo-Ona, C.M., Avarenga, M.A., De.Vita, B., Trinque, C., Puoli-Filhob, J.N.D. & Dell Aqu, J.A. (2010). Replacing egg yolk with soybean lecithin in the cryopreservation of stallion semen. Journal of Animal Reproduction Science, 129, 73-77.
  41. Partyka, A., Niżański, W. & Łukaszewicz, E. (2010). Evaluation of fresh and frozen-thawed fowl semen by flow cytometry. Theriogenology, 74, 1019-1027.
  42. Pruchniak, M.P., Arzna, M. & Demkow, U. (2016). Biochemistry of oxidative stress. Advances in Experimental Medicine and Biology, 878, 9–19.
  43. Rengaraj, D. & Hong, Y.H. (2015). Effects of dietary vitamin E on fertility functions in poultry species. International Journal of Molecular Sciences, 16(5), 9910-9921.
  44. Rutkowska, J., Cichon, M., Puerta, M. & Gil, D. (2005). Negative effects of elevated testosterone on female fecundity in zebra finches. Hormones and Behavior, 47, 585-591.
  45. Safari Asl, R., Shariatmadari, F., Sharafi, M., Karimi Torshizi, M.A. & Shahverdi, A. (2018). Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Ross breeder roosters fed a diet supplemented with a moderate ratio of n-3: n-6 fatty acids. Poultry Science, 97(11), 4113-4121.
  46. Sanocka, D. & Kurpisz, M. (2004). Reactive oxygen species and cells. Reproductive Biology and Endocrinology, 2(12), 1-7.
  47. Sexton, K.J. and Renden J.A. (1988). Effects of feeding regimen during early development on body composition, gastro-intestinal tract size and semen quality of broiler breeder cockerels after maturation. Poultry Science, 67, 835-841.
  48. Shahverdi, A., Sharafi, M., Gourabi, H., Amiri Yekta, A., Esmaeili, V., Sharbatoghli, M., Janzamin, E., Hajnasrollahi, M. & Mostafayi, F. (2015). Fertility and flow cytometric evaluations of frozen-thawed rooster semen in cryopreservation medium containing low-density lipoprotein. Theriogenology, 83, 78-85.
  49. Surai, P.F., Fujihara, N., Speake, B.K., Brillard, J.P., Wishart, G.J. & Sparks, N.H.C. (2002). Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semen. Asian-Australas Journal of Animal Science, 14, 1024–1050.
  50. Surai, P.F., Kochish, I.I., Romanov, M.N. & Griffin, D.K. (2019). Nutritional modulation of the antioxidant capacities in poultry: the case of vitamin E. Poultry science, 98(9), 4030-4041.
  51. Tabatabaei, S., Batavani, R.A. & Ayen, E. (2011). Effects of vitamin e addition to chicken semen on sperm quality during in vitro storage of semen. Veterinary Research Forum, 2(2), 103-111.
  52. Teymouri Zadeh, Z., Shariatmadari, F., Sharafi, M. & Amir Torshizi, M.M. (2020). Amelioration effects of n-3, n-6 sources of fatty acids and rosemary leaves powder on the semen parameters, reproductive hormones, and fatty acid analysis of sperm in aged Ross broiler breeder roosters. Poultry Science, 99(2), 708-718.
  53. Tsakmakidis, I.A. (2010). Ram semen evaluation: development and efficiency of modern Small Ruminants Research, 92(1), 126-130.
  54. Tsukunaga, S. (1987). Morphological evidence of osmotic and thermal shock of fowl sperm in relation to infertility of sperm. Bulletin of the Hiroshima Agricultural College, 8, 257-303.
  55. Wang, G. and Wang, T. (2008). Oxidative stability of egg and soy lecithin as affected by transition metal ions and pH in emulsion. Journal of Agricultural and Food Chemistry, 56, 11424-11431.
  56. Weil, S., Rozenboim, I., Degen, A., Dawson, A., Friedländer, M. & Rosenstrauch, A. (1999). Fertility decline in aging roosters is related to increased testicular and plasma levels of estradiol. General and Comparative Endocrinology, 115, 23-28.
  57. Zaniboni, L. & Cerolini, S. (2009). Liquid storage of turkey semen: Changes in quality parameters, lipid composition and susceptibility to induced in vitro peroxidation in control, n-3 fatty acids and alphatocopherol rich spermatozoa. Animal Reproduction Science, 112, 51-65.
  58. Zanussi, H.P., Shariatmadari, F., Sharafi, F. & Ahmadi, H. (2019). Dietary supplementation with flaxseed oil as source of Omega-3 fatty acids improves seminal quality and reproductive performance in aged broiler breeder roosters. Theriogenology, 130, 41-48.
  59. Zini, A., de Lamirande, E. & Gagnon, C. (1993). Reactive oxygen species in the semen of infertile patients: Levels of superoxide dismutase and catalase-like activities in seminal plasma. International Journal of Andrology, 16, 183-188.