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تأثیر سطوح بالا آلفا- توکوفرول استات بر فراسنجه‌های بافت‌شناسی بیضۀ خروس‌های سنگین‌ مادر گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشجوی سابق دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

هدف از این بررسی ارزیابی تأثیر افزودن سطوح بالای آلفاتوکوفرول استات بر فراسنجه‌های بافت­شناسی بیضۀ خروس‌های سنگین مادر گوشتی بود. در این راستا، شمار 60 خروس مادر گوشتی راس 308 (با سن 20 هفته) در دو گروه وزنی استاندارد (30=شمار؛ kg169/0±109/3=میانگین وزن بدن) و سنگین (30=شمار؛ kg 161/0 ± 509/3= میانگین وزن بدن) تقسیم شدند. خروس­ها به‌صورت تصادفی در ده گروه تیماری تقسیم و به قفس­های انفرادی منتقل شدند (به‌صورت فاکتوریل 5×2 با شش تکرار در هر تیمار). تیمارهای آزمایشی با جیره­ای همسان افزون بر سطوح مختلف ویتامین E 0 (E0)، ۱۰۰ (E100)، ۲۰۰ (E200)، ۳۰۰ (E300)، ۴۰۰ (E400) میلی‌گرم در کیلوگرم جیرۀ ویتامین E به مدت ده هفته تغذیه شدند. در مدت آزمایش، گروه سنگین 30 درصد بیشتر از گروه سبک خوراک دریافت کرد. در پایان آزمایش خروس­ها کشتار شدند و بیضه­ها برای ارزیابی­های بافت­شناسی از لاشه جدا شدند. نتایج نشان داد، ویتامین E به‌صورت معنی­داری اندازۀ بیضه­ها و شمار لوله­های اسپرم‌ساز را در همۀ گروه­های تیماری نسبت به گروه شاهد (کنترل) افزایش داده است (05/0P≤). همچنین، در هر دو گروه وزنی، قطر بیضۀ چپ، قطر لوله­های اسپرم­ساز، ضخامت بافت پوششی (اپیتلیوم) اسپرم­ساز، شمار یاخته­های لایدیگ و شمار رگ‌های خونی برای گروه 400 میلی­گرم ویتامین E بالاتر بود (05/0P≤).  درکل، بنابر نتایج به‌دست‌آمده می­توان عنوان کرد، سطوح بالای ویتامین E (400 میلی­گرم در کیلوگرم جیره) موجب بهبود فراسنجه‌های بافت‌شناسی بیضۀ خروس شده است.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of high doses of α-tocopherol acetate on testicular morphology and histology parameters in broiler breeder roosters

نویسندگان [English]

  • Majid Ajafar 1
  • Mojtaba Zaghari 2
  • Mehdi Zhandi 3
  • Leila Lotfi 4
1 Ph. D. Candidate, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Former Ph.D. Student, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The aim of this study was to investigate effect of dietary supplementation by high doses of α-tocopherol acetate breeder (20 weeks of age) were used in two groups, standard (n=30; body weight= 3.109±0.169) and heavy (n=30; ±body weight= 3509±0.161) weight. Roosters were individually kept and randomly divided into 10 treatments (in a 2×5 factorial arrangements with six replicates in each group).  The experimental treatments were fed a same diet supplemented with different levels of vitamin E (0, 100, 200, 300, 400 mg α-tocopherol acetate/kg diet) for 10 weeks. The heavy weight group received 30% excessive diet than standard group from beginning until the end of the experiment. After 5 weeks of adaptation period, roosters were fed by supplemented diet for 10 weeks. In the end of experiment roosters slaughtered and testicles removed from carcass for morphology and histology analysis. Results showed that vitamin E were significantly increased the size of testis, the number of seminiferous tubules in all treatment group compared to the control group (P ≤ 0.05). As well as, in two body weight groups the weight of testis, left testis diameter, seminiferous tubules diameter, seminiferous epithelium thickness, number of leydig cell and blood vessels were highest in E400 group (P ≤ 0.05). Finally, based on these results, it was concluded that high dose of vitamin E (400 mg/kg diet) improved testis morphology and histology parameters.

کلیدواژه‌ها [English]

  • Heavy weight roosters
  • leydig cells
  • Seminiferous tubules
  • seminiferous epithelium
  • vitamin E
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