Azadeh Mirshamsollahi; Mahdi Ganjkhanlou; Farhang Fatehi; Abolfazl Zali; Mostafa Sadeghi
Abstract
An experiment was performed to investigate the effects of using saturated and unsaturated fatty acids calcium salts on the production performance and some physiological traits of finishing lambs fattened under heat stress condition. Thirty-two cross bred Lori-BakhtiariÍRomanov lambs were randomly ...
Read More
An experiment was performed to investigate the effects of using saturated and unsaturated fatty acids calcium salts on the production performance and some physiological traits of finishing lambs fattened under heat stress condition. Thirty-two cross bred Lori-BakhtiariÍRomanov lambs were randomly divided into 4 groups. Lambs were fed individually with the following experimental diets for 6 weeks (early July to mid-August): 1) Basal diet without supplemental fat (control), 2) Basal diet supplemented with 2% (on DM basis) of fish oil calcium salt, 3) Basal diet supplemented with 2% (on DM basis) of olive oil calcium salt and 4) Basal diet supplemented with 2% (on DM basis) of saturated fat calcium salt. Daily feed intake was recorded and lambs body weight was measured weekly. Rectal temperature and respiration rate were measured once per week. Using calcium salts of fish oil, olive oil and saturated fats in the diet significantly increased daily weight gain and reduced the lambs feed conversion ratio compared to control group (P≤ 0.05), but had no effect on dry matter intake of the Lambs. Regardless of fatty acid origin, supplementing lambs with calcium salts of fatty acids, reduced rectal temperature and respiration rate of lambs exposed to heat stress, compared to control group (P≤ 0.01). Also, using calcium salts of Fish oil, olive oil and saturated fat had no effect on DM, CP and EE digestibility, however, EE digestibility was higher in lambs consumed fat-supplemented diets than the control group. NDF digestibility was significantly higher in the control group lambs than the other groups. The results of this study showed that diet supplementation with calcium salts of unsaturated fatty acids of fish and olives and saturated fatty acids, improved the production performance of finishing lambs exposed to heat stress. No difference was detected between fat supplements with different fatty acid patterns, in this investigation.
Farzad Ghafouri; Mostafa Sadeghi; Abolfazl Bahrami; Rostam Abdollahi-Arpanahi; Arash Javanmard; Seyed Reza Miraei-Ashtiani
Abstract
Genetic selection for increasing milk production and economic profitability in the dairy industry has been associated with reduction in reproductive performance, including lower embryo survival and pregnancy loss. The main purpose of this study was to use the transcriptome profiles of endometrial tissue ...
Read More
Genetic selection for increasing milk production and economic profitability in the dairy industry has been associated with reduction in reproductive performance, including lower embryo survival and pregnancy loss. The main purpose of this study was to use the transcriptome profiles of endometrial tissue and Corpus luteum of two groups of high and low fertility Holstein dairy cows to identify genes that are effective in reproductive rate, especially in early pregnancy. By the analysis of RNA-Seq data to express the gene differences, 4538 genes were extracted, which a total of 1466 genes showed significant expression differences (P<0.000001, Fold change<0.5). Then, by comparing the relevant genes among transcriptome profiles, common genes between endometrial tissue (on days 7 and 13 of the estrous cycle) and Corpus luteum (on day 13 of the estrous cycle) including SYNM, PARM1, NXPE2, NT5DC3, COL4A3, COL12A1, ALPK3, ADAMDEC1, SERPINA14, S100A9, PI16, OAS1X, MSTN, MASP1, CD83, CA2, C2, C5, JSP.1 and SAA3 were identified. Annotation results of these genes indicated that they have a role in the main process of metabolic and signaling pathways related to the ion transport system, inflammation, immune system function, and cell-matrix structure. Overall, the present study can provide new insights into the molecular evidence for the biological mechanisms of transcriptome profiling in the uterine environment and biomarkers related to fertility in dairy cows.
Reza Faraji; Ardeshir Nejati Javaremi; Mostafa Sadeghi; Reza Miraei Ashtiani; Hamid Reza Mozhgani
Abstract
Nowadays one of the major challenges facing herders, dog owners, or working dog training centers is canine parvovirus (CPV-2). The virus has split into several types over the years since it discovered and resulted to genetic and amino acid changes that threaten a wide range of carnivorous around the ...
Read More
Nowadays one of the major challenges facing herders, dog owners, or working dog training centers is canine parvovirus (CPV-2). The virus has split into several types over the years since it discovered and resulted to genetic and amino acid changes that threaten a wide range of carnivorous around the world. The best way to avoiding the consequences of this disease is vaccination. This study were designed, due to the lack of sufficient studies on the distribution and prevalence of different types of this virus, which may be a prominent cause of vaccine errors, also because of high involvement of Iranian dogs with CPV. Molecular study and phylogenetic comparison of Iranian types compared to other types through the world showed that the dominant type of CPV-2 existed in Iran is CPV-2a with a prevalence of 24% and genetic differences in several points of VP2 gene with other types. We are probably facing to a new version of this virus in our country. In addition, based on the drawn phylogenetic tree, it was found that Iranian isolates on the other side have a relatively high topological difference compared to the origin types and conventional vaccines strain, which supports the initial hypothesis of this study.
Milad Rezaei Sinaki; Mostafa Sadeghi; Abolfazl Bahrami; Mohammad Moradi Shahrbabak
Abstract
Heat stress in poultry decreases performance, weakens immune system and increases mortality, significantly. Given the interactions between biological pathways involved in heat stress, it is necessary to use a comprehensive approach to study heat stress. In this study, the effects of heat stress on gene ...
Read More
Heat stress in poultry decreases performance, weakens immune system and increases mortality, significantly. Given the interactions between biological pathways involved in heat stress, it is necessary to use a comprehensive approach to study heat stress. In this study, the effects of heat stress on gene expression in two groups of broilers under heat stress and without heat stress (control) were investigated. In the analysis, microarray data were extracted from 1000 genes and after removing duplicate genes and out of the level of significance in expression (P <0.01), 709 genes were identified. Using the String site and gene analysis in Cytoscape software, 115 genes were identified in four functional modules. The identified modules were involved in biological pathways of Spliceosome, Ubiquitin-mediated proteolysis, Ribosome biogenesis, Protein Processing in Endoplasmic Reticulum, Autophagy-Animal and Important Signaling pathways including Innate Immune System, MAPK pathway and Cellular Senescence. The results of this study showed that heat stress in poultry plays an important role in growth function, immune system and other biological mechanisms. Identifying the genes involved in heat stress such as PTEN and HSPs in birds, and reviewing microarray data could open new horizons for a better understanding heat stress-related biological process.
Masoud Barzegar; Mojtaba Zaghari; Mahdi Zhandi; Mostafa Sadeghi
Abstract
In this research the effects of different levels and particle sizes of zinc oxide on reproductive performance of hens and roosters of broiler breeders were evaluated. A total of 200 female Ross 308 broiler breeders in a completely randomized design with 4 treatments, 5 replicates and 5 birds per each ...
Read More
In this research the effects of different levels and particle sizes of zinc oxide on reproductive performance of hens and roosters of broiler breeders were evaluated. A total of 200 female Ross 308 broiler breeders in a completely randomized design with 4 treatments, 5 replicates and 5 birds per each replication, and 24 male Ross 308 broiler breeders in a completely randomized design with 4 treatments, 6 replicates and one bird per each replication at 54 weeks of age were used. The experimental treatments included the diets containing 70 mg zinc from zinc oxide with large particle size (LPZnO-70), 100 mg zinc from zinc oxide with large particle size (LPZnO-100), 70 mg zinc from zinc oxide with small particle size (SPZnO-70) and 100 mg zinc from zinc oxide with small particle size (SPZnO-100). The results indicated that the experimental treatments had no significant effect on egg production, body weight, egg weight and egg yolk weight. In the entire experimental period, the egg shell thickness was significantly higher in treatment containing SPZnO than LPZnO (P<0.001). The highest and lowest fertility and hatchability ratioes were observed in treatments containing SPZnO-100and LPZnO-70, respectively. The highest semen volume was observed in roosters of treatments containing SPZnO-100 and LPZnO-100 (P< 0.05). Generally, it can be concluded that SPZnO has a more desirable effect on reproductive parameters of broiler breeders due to its higher bioavailability. Since the level of 100 mg zinc per kg of diet from both sources of zinc oxide resulted in better reproductive performance, the utilization of SPZnO-100 might be recommended.
Muna Kholghi; Mohammad Moradi Shahre Babak; Mostafa Sadeghi; Seyed Reza Miraei-Ashtiani; Mohammad Mehdi Ranjbar; Mohsen Lotfi
Abstract
This study was conducted to evaluate prevalence of BVD and the strategies which were applied for prevention of BVD over the past 50 years in some provinces of Iran. Blood samples of 500 Iranian Holstein race were randomly collected from three dairy farms located in Tehran, Isfahan and Qazvin provinces. ...
Read More
This study was conducted to evaluate prevalence of BVD and the strategies which were applied for prevention of BVD over the past 50 years in some provinces of Iran. Blood samples of 500 Iranian Holstein race were randomly collected from three dairy farms located in Tehran, Isfahan and Qazvin provinces. BVD control protocols of these farms were recorded. ELISA technique was used to measure the antibody and antigen titers for BVD. RT-PCR technique was performed to investigate the presence and the type of virus in all samples. The prevalence of Ab-/ Ag-, Ab+/ Ag-, Ab-/ Ag+ and Ab-/ Ag+ were 10.2%, 78.8%, 7.2% and 3.8%, respectively. Furthermore, approximately 4.2% of the animals were PI and the prevalence of antibody and antigen titers had not significant difference in three provinces. All positive samples were BVDV type 1. Type 2 was not observed in this study. The results of the study indicated that the efficiency of the used protocols to control BVD diseases is not successful. So, to control the BVD, applying large management policies at the national level is fundamental beside the biosecurity strategies.
Farzad Ghafouri; Mostafa Sadeghi; Abolfazl Bahrami; Seyed Reza Miraei Ashtiani
Abstract
Abdominal fat deposition and several other unique features in the metabolism of birds such as interaction between genetic and endocrine factors, fasting hyperglycemia and insulin resistance are signs of obesity and metabolic disorders in poultry, similar to humans. The main purpose of this study was ...
Read More
Abdominal fat deposition and several other unique features in the metabolism of birds such as interaction between genetic and endocrine factors, fasting hyperglycemia and insulin resistance are signs of obesity and metabolic disorders in poultry, similar to humans. The main purpose of this study was to use transcript profile of fat tissue in two groups of broiler chickens with high and low abdominal fat deposition, to identify the genes involved in storage and metabolism of fat, as well as the signaling pathways associated with the endocrine glands. Based on the analysis of microarray and RNA-seq data, 2914 and 1867 genes were detected as differentially expressed genes, respectively. In total, 1835 genes were identified by comparing the genes with a significant difference in expression (P<0.000001). Then, by comparing the number of relevant genes among the transcript profiles, the most important related genes were THBS1, COLEC12, ANXA7, RGS19, TMEM258 and HTR7L, which in the main process of pathways controlling synthesis, fat metabolism and storage and the endocrine signaling pathways activated by adipokines, are involved. The analysis of the relevant tissue may indicate the role of ventricular fat as a metabolic and endocrine organ in broiler chickens.
Mahdi Mokhber; Mohammad Moradi Shahre Babak; Mostafa Sadeghi; Hossein Moradi Shahrbabak; Javad Rahmani-Nia
Abstract
In order to estimate the effective population size (Ne) in Iranian water buffalo blood and hair samples of 407 individual from Azari (N=260), Khuzestani (N=120) and Mazandrani (N=27) buffalo populations were gathered. After DNA extaraction, the samples were genotyped using Axiom® Buffalo Genotyping ...
Read More
In order to estimate the effective population size (Ne) in Iranian water buffalo blood and hair samples of 407 individual from Azari (N=260), Khuzestani (N=120) and Mazandrani (N=27) buffalo populations were gathered. After DNA extaraction, the samples were genotyped using Axiom® Buffalo Genotyping 90K Array. The Ne was estimated from 700 to 4 generations ago and also for the present generation by linkage disequiblirum data and based on heterozygote-excess method using NeEstimator (V2), respectively. Estimated Ne for Azari, Khuzestani and Mazandarani were calculated 1530, 1375 and 1141, respectively, for 700 generations ago. Ne for the present generation in Azeri, Khuzestani and Mazandarani were estimated 447, 226 and 35, respectively. The Ne for Azeri and Khuzestani were relatively high and these two populations were not endanger to extinction, but their Ne has been declined in the resent generations massively and it is necessary to care about the maintenance of Ne and relatively high diversity for these populations. However, the Mazandarani population is endangered because of low Ne and so it is necessary to carefully monitor their effective population size, improve the profitability of production and planning a suitable mating scheme to control inbreeding and genetically conserve this population.
Mahdi Mokhber; Mohammad Moradi Shahre Babak; Mostafa Sadeghi; Hossein Moradi Shahrbabak; Javad Rahmani-Nia
Abstract
In order to detect signature of selection on buffalo genome, a set of 287 water buffalo samples from 260 Azari and 27 Mazandarani buffalo breeds were genotyped using the Axiom® Buffalo Genotyping 90K Array. The unbiased fixation index method (FST) was used to detect signatures of selection. In total, ...
Read More
In order to detect signature of selection on buffalo genome, a set of 287 water buffalo samples from 260 Azari and 27 Mazandarani buffalo breeds were genotyped using the Axiom® Buffalo Genotyping 90K Array. The unbiased fixation index method (FST) was used to detect signatures of selection. In total, 14 regions with outlier FST values (0.1%) were identified. Annotation of these regions using the UMD3.1 Bos taurus Genome Assembly was performed to find putative candidate genes and QTLs within the selected and 105 genes and 28 QTLs with selection signatures were detected. A high proportion of identified genes (N=27) in regions under selection were involved in olfactory receptor, also some of the detected genes were associated with growth and body development, metabolicand apoptosis possesses, immune system development, and mammary gland development. Some of the identified QTLs in regions under selection were associated with growth traits such as body weight at birth, weaning and mature, subcutaneous fat, meat yield and carcass weight. The detected QTL for milk traits were only associated with milk contents and somatic cell count. However, it is recommended to carry out association studies to show the actual function of these genes.
Mahdieh Yousefi Daresytani; Sayed Reza Miraie-Ashtiani; Mostafa Sadeghi
Abstract
Considering the importance of maintaining the diversity in native breeds and identification of genetic resources of the country, the genetic diversity in Azari buffaloes was evaluated using ten microsatellite markers (BoLA-DRB3, BM1818,BM1824,CSSM033,CSSM047,ILSTS017,ILSTS033,ILSTS058,ILSTS061andILSTS089). ...
Read More
Considering the importance of maintaining the diversity in native breeds and identification of genetic resources of the country, the genetic diversity in Azari buffaloes was evaluated using ten microsatellite markers (BoLA-DRB3, BM1818,BM1824,CSSM033,CSSM047,ILSTS017,ILSTS033,ILSTS058,ILSTS061andILSTS089). Hair samples were taken from 70 Azari buffaloes from three regions of the northwest of Iran including Ardabil, East Azerbaijan and West Azerbaijan provinces. Genomic DNA was extracted from the hair samples by the modified salting-out method and for DNA amplification the polymerase chain reactions for all primers were optimized. The results indicated that, in Azari buffalo population, all microsatellites loci were highly polymorphic, and they were deviated from Hardy-Weinberg equilibrium (P<0.001) with an exception for BoLA-DRB3 locus. The average observed and expected allelic numbers were 5.9 and 4.72, respectively. The observed and expected heterozygosity, Polymorphic Information Content (PIC) and Shannon Index were 0.87, 0.75, 0.71 and 1.57, respectively. In conclusion, the genetic variation in Azeri buffalo population is quite high and reliable.
Nahid Parna; Ardeshir Nejati Javaremi; Mostafa Sadeghi; Rostam Abdollahi Arpanahi
Abstract
Using SNP markers information and genomic evaluation approach, predicting the genetic merit of individuals without phenotypic records is now possible. However, using high-density panels for genomic evaluation of all individuals is not economically feasible. To achieve high genomic prediction accuracy ...
Read More
Using SNP markers information and genomic evaluation approach, predicting the genetic merit of individuals without phenotypic records is now possible. However, using high-density panels for genomic evaluation of all individuals is not economically feasible. To achieve high genomic prediction accuracy with reasonable price, it is possible to genotype a proportion of animals with high-density panels and the rest of animals with low-density panels then impute them to high-density genotypes. In this study, the effect of three low-density panels (1k, 2k and 4k), genotype imputation to 10k panel and the relationship between reference and validation populations on the accuracy of genomic predictions and also the correlation between the estimated breeding values using panels with different densities in simulated data were assessed. The low density panels genotypes were actually consisting of 10, 20 and 40 percent of 10k markers selected randomly and FImpute was used for genotype imputation. As a general trend, by increasing the density of markers, the correlation between the estimated breeding values was increased using different panels. So, the accuracy of genomic predictions was similar using 4k and 10k genotypes. Moreover, imputing 4k to 10k genotypes, did not improve the accuracy of genomic prediction. However, the accuracy of estimated breeding values was increased after imputation from 1k or 2k to 10k. The accuracy of imputation was decreased when the reference and validation populations were more distant.
Motahareh Ala Amjadi; Hassan Mehrabani yegahneh; Mostafa Sadeghi
Abstract
In this research genetic variation using 6 microsatellite loci (HMS07, HMS03, HMS02, HMS06, ASB02, ASB23) was studied in Iranian Kurdish horse population .Whole blood samples were randomly collected from 52 horses in Kurdistan, Kermanshah, Elam, ...
Read More
In this research genetic variation using 6 microsatellite loci (HMS07, HMS03, HMS02, HMS06, ASB02, ASB23) was studied in Iranian Kurdish horse population .Whole blood samples were randomly collected from 52 horses in Kurdistan, Kermanshah, Elam, Western Azerbaijan, Esfahan, Kerman and Hamadan regions. DNA was extracted from blood samples. Polymerase chain reaction (PCR) was used for amplification of 6 microsatellite loci using pairs of standard specific primers. Then, the PCR products assessed with 8% silver-staining acrylamide gel and electrophoresed. All microsatellites loci were polymorphic. The number of alleles observed and expected hetrozygosities were calculated with Shannon Index. The largest and smallest number of alleles were observed in HMS07 locus (10 alleles) and HMS02 (4 alleles) respectively. Our results confirmed the high efficiency of microsatellite markers for assessing population structure in Iranian native horses.
Abolfazl Bahrami; Seyed Reza Miraie-Ashtiani; Mostafa Sadeghi; Ali Najafi
Abstract
At the later stage of folliculogenesis, the mammalian ovarian follicle contains layers of epithelial granulosa cells surrounding an antral cavity. During follicle development, granulosa cells replicate, secrete hormones and support the growth of the oocyte. In cattle, the follicle needs to grow over ...
Read More
At the later stage of folliculogenesis, the mammalian ovarian follicle contains layers of epithelial granulosa cells surrounding an antral cavity. During follicle development, granulosa cells replicate, secrete hormones and support the growth of the oocyte. In cattle, the follicle needs to grow over 10 mm in diameter to allow an oocyte release in ovulation process, following which the granulosa cells cease dividing and differentiate into the specialized cells of the corpus luteum. To better understand the molecular basis of follicular growth and granulosa cell maturation, we undertook the transcriptome profiling of granulosa cells from small (< 5 mm; n = 10) and large (> 10 mm, n = 4) healthy bovine follicles, using data mining. In this regard, we have studied important genes that are included in folliculogenesis process using data, freely available in the different databases. In total 283 genes were identified with the comparison of transcriptome profiling of large and small granulosa cells. With construction and analysis of network, we became able to identify the interaction between them and finally we have found 6 important and functional modules using various software. The most important genes involved, were TNFα, NR1H4, LHCGR, FSHR, PTHLH, LHB, CAD, HSD3B1, CYP17A1, DICE1, MCE1, COX and Aromatase. These results suggest that identified modules can be used as markers for follicle differentiation and apoptosis process.
Masoumeh Naserkheil; Seyed Reza Miraie-Ashtiani; Mostafa Sadeghi; Ardeshir Nejati-Javaremi; Deukhwan Lee
Abstract
Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition ...
Read More
Diacylglycerol acyltransferase1 (DGAT1) is a key enzyme in synthesis of triglycerides. A transition mutation resulting substitution of guanine by adenine in DGAT1 gene and substitution of lysine by alanine in diacylglycerol-acyltransferase enzyme in cattle has a major effect on milk yield and milk composition traits. In this research, the polymorphism of the region spanning exon 3 to exon 17 of the DGAT1 gene of three indigenous Iranian buffalo populations was investigated. A total number of samples of 200 buffaloes (Khuzestani, Shomali and Azari) were collected to extract DNA and the desired fragments were amplified by polymerase chain reaction (PCR). Genotyping was performed using direct sequencing and analyzed by BioEdit software. Sequence analysis showed novel SNPs in comparative to the reference sequence GenBank (DQ886485) at nucleotide positions g.6097A>G, g.7036C>T, g.7338G>A, g.7710C>T, g.8087C>T, g.8259G>A, g.8275G>A and g.8426C>T of DGAT1 gene in buffalo populations. A comparison with Indian buffalo revealed three exonic SNPs, one of which was non-synonymous. A Unique 22 base insertion has been observed in the intron 10. The results described here genetic diversity and could be useful in genetic and breeding programs in Iranian buffalo.
Hossein Moradi Shahrbabak; Rasoul Khodabakhshzadeh; Mostafa Sadeghi; Amir TaheriYeganeh
Abstract
The growth hormone (GH) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. Growth hormone can contribute in growth metabolism when it\'s receptor (GHR) are on cells target. The single nucleotide polymorphism was occurred in GH and GHR genes and that ...
Read More
The growth hormone (GH) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. Growth hormone can contribute in growth metabolism when it\'s receptor (GHR) are on cells target. The single nucleotide polymorphism was occurred in GH and GHR genes and that associated with carcass traits in Lori-Bakhtiari sheep breed with using PCR-SSCP. In this research, blood samples were collected from the left jugular vein from 152 Lori-Bakhtiari sheep breed. Genomic DNA was extracted from blood samples by using the salting-out procedure and PCR were used to amplify the regions are located in exon 4 (214 bp segments) of the ovine GH gene and exon 10 (218 bp segments) of the ovine GHR Gene. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using acrylamide gel electrophoresis and silver-nitrate staining method. The result showed polymorphism in GH gene but any difference between banding patterns for GHR gene was not observed. The sequencing results showed the presence of 5 Single nucleotide polymorphism for GH gene in the studied population. No significant associations of the available genotypes in the exon 4 of the ovine GH Gene with carcass traits.
Mahdi Ansari; Mahdi Zhandi; Hamid Kohram; Mojtaba Zaghari; Mostafa Sadeghi
Abstract
The aim of this study was to evaluate the effect of D-aspartic acid on semen characteristics of Ross 308 roosters. Thirty 60-wk-old Ross 308 roosters were randomly assigned into 6 groups and individually caged. All groups fed the same basal diet and orally administered with different levels of D-aspartic ...
Read More
The aim of this study was to evaluate the effect of D-aspartic acid on semen characteristics of Ross 308 roosters. Thirty 60-wk-old Ross 308 roosters were randomly assigned into 6 groups and individually caged. All groups fed the same basal diet and orally administered with different levels of D-aspartic acid: 0(A-0), 40 (A-40), 80 (A-80), 120 (A-120), 160 (A-160), and 200 (A-200) mg/Kg BW per day as a single capsulated dose. Seminal characteristics including ejaculate volume, motility, progressive motility, sperm concentration, abnormality, viability and hypo osmotic swimming test were studied following two weeks of adaptation period, and continued for up to six weeks. Results of the experiment revealed that D-Aspartic acid positively affected rooster sperm parameters with all parameters being significantly improved with increasing the level of D-aspartic acid except for abnormality percentage that was not significantly affected by the treatment (p>0.05). Taking all parameters into account, A-200 treatment exhibited the most optimal response in sperm parameters amongst all treatments. However, further studies including fertility and hatchability tests are needed to ascertain these results.
Reza Faraji; Mostafa Sadeghi; Mohammad Moradi Shahrbabak
Abstract
This research was conducted to study polymorphism of intron 44 of ACACA gene and its association with milk traits in Mahabadi goats. Blood samples were taken from 81 Mahabadi goats. DNA was extracted from blood using salting out method. 897 base pair fragment of intron 44 ACACA gene was amplified using ...
Read More
This research was conducted to study polymorphism of intron 44 of ACACA gene and its association with milk traits in Mahabadi goats. Blood samples were taken from 81 Mahabadi goats. DNA was extracted from blood using salting out method. 897 base pair fragment of intron 44 ACACA gene was amplified using polymerase chain reaction. SSCP method was used to investigate the amplified DNA fragment polymorphism. Amplified products were sequenced in both directions. Finally, seven different patterns were obtained and sequences compared with each other also with Capra hircus ACACA gene sequence on NCBI (NC_022311). Seven novel SNPs were identified which were NC_022311: nucleotide 154503(T/G), 154518(A/G), 154577(A/G), 154640(C/T), 154712(A/C), 154928(C/T), 154956(C/T). Then a mixed animal model was used by SAS9.1 analysis system to evaluate the association of these SNPs with milk traits. In this model, variables of age of dam, genotypes and mount of recording were considered as fixed effects. There were significant association of SNP2 (P=0.05), SNP3 (P=0.05) and SNP5 (P<0.01) with milk yield. SNPs 1 (P=0.017), 6 (P=0.017) and 7 (P<0.01) had also significant effect on milk fat-percentage.
Mahdi Mokhber; Mohammad Moradi Shahrbabak; Mostafa Sadeghi; Hossein Moradi Shahrbabak; John Williams
Abstract
Identification of selection targeted genomic regions is one of the most challenging areas of research in animal genetics, particularly in livestock. We carried out a genome-wide scan for signatures of positive selection to identify genomic regions that had been under selection in Iranian Khuzestani and ...
Read More
Identification of selection targeted genomic regions is one of the most challenging areas of research in animal genetics, particularly in livestock. We carried out a genome-wide scan for signatures of positive selection to identify genomic regions that had been under selection in Iranian Khuzestani and Mazandrani buffalo breeds. A total of 148 water buffalo from Khuzestani (N=121) and Mazandrani (N=27) buffalo breeds were genotyped using Axiom® Buffalo Genotyping 90K Array. Unbiased method of population differentiation index (FST) was applied to detect signatures of selection. In total, 23 regions exceeding the 0.1 percent threshold of the empirical posterior distribution were identified as extremely differentiated. These selected genomic regions were surveyed to find encoding putative candidate genes and 64 genes and 27 QTL were extracted from the corresponding areas in UMD3.1 Bos Taurus Genome Assembly. Some of these genes have previously reported as signature of positive selection in the last studies. Some of these genes were also found to be involved in milk production traits and domestication-related changes include sensory perceptions, brain and neural system development, pigmentation, and geographic adaptation. Also, survey on extracted QTLs was shown that these QTLs involved in some economicl important traits in buffalo such as feed conversion ratio, subcutaneous fat, body weight, average daily gain, type, Meat tenderness, milk production conentent, udder attachment, calf size and calving ease traits. However, it will be necessary to carry out more association and functional studies to demonstrate the implication of these genes.
