Identification of genes, biological pathways and signaling affecting heat stress with ‎microarray data sets in poultry

Document Type : Research Paper


1 M.Sc. Student of Animal Breeding and Genetics, Department of Animal Science, ‎College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor of Animal Breeding and Genetics, Department of Animal Science, College of ‎Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Former Ph.D. Srtudent of Animal Breeding and Genetics, Department of Animal Science, College of Agriculture and Natural ‎Resources, University of Tehran, Karaj, Iran

4 Professor of Animal Breeding and Genetics, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, ‎Karaj, Iran


Heat stress in poultry decreases performance, weakens immune system and increases mortality, significantly. Given the interactions between biological pathways involved in heat stress, it is necessary to use a comprehensive approach to study heat stress. In this study, the effects of heat stress on gene expression in two groups of broilers under heat stress and without heat stress (control) were investigated. In the analysis, microarray data were extracted from 1000 genes and after removing duplicate genes and out of the level of significance in expression (P <0.01), 709 genes were identified. Using the String site and gene analysis in Cytoscape software, 115 genes were identified in four functional modules. The identified modules were involved in biological pathways of Spliceosome, Ubiquitin-mediated proteolysis, Ribosome biogenesis, Protein Processing in Endoplasmic Reticulum, Autophagy-Animal and Important Signaling pathways including Innate Immune System, MAPK pathway and Cellular Senescence. The results of this study showed that heat stress in poultry plays an important role in growth function, immune system and other biological mechanisms. Identifying the genes involved in heat stress such as PTEN and HSPs in birds, and reviewing microarray data could open new horizons for a better understanding heat stress-related biological process.


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