اثر پپتیدهای استخراج شده از کنجاله تخم آفتابگردان بر عملکرد، ویژگی‌های لاشه و فعالیت آنتی‌اکسیدانی در جوجه‌های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 'گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

2 گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران

چکیده

این آزمایش به منظور بررسی اثر پپتیدهای استخراج‌ شده از کنجاله تخم ‌آفتابگردان بر عملکرد، ویژگی‌های لاشه و فعالیت آنتی‌اکسیدانی در جوجه گوشتی انجام شد. در این آزمایش از 200 قطعه جوجه گوشتی نر سویه راس 308 در قالب طرح کاملا تصادفی با 5 تیمار و 4 تکرار و 10 قطعه جوجه در هر تکرار،  به مدت 26 روز استفاده شد. تیمارهای آزمایشی شامل: 1) جیره شاهد بدون افزودنی 2) جیره‌ شاهد + 300 میلی‌گرم ویتامین ای در کیلوگرم جیره 3) جیره‌ شاهد + 250 میلی‌گرم پپتید استخراج ‌شده‌ از کنجاله تخم آفتابگردان در کیلوگرم جیره 4) جیره‌ شاهد + 500 میلی‌گرم پپتید استخراج ‌شده‌ از کنجاله تخم آفتابگردان در کیلوگرم جیره  5) جیره‌ شاهد + 1000 میلی‌گرم پپتید استخراج ‌شده‌ از کنجاله تخم آفتابگردان در کیلوگرم جیره بودند. از نظر مصرف خوراک و افزایش وزن در دوره‌ رشد ( 26-11 روزگی) و در کل دوره آزمایش (26-1 روزگی)، اختلاف معنی‌داری بین تیمارها مشاهده شد ( 05/0<  (P. بیشترین و کمترین مقدار مصرف خوراک و افزایش وزن، به ترتیب مربوط به تیمار 5 و تیمار شاهد بود. کمترین مقدار ضریب تبدیل خوراک در دوره‌ رشد و در کل دوره، مربوط به تیمار 5 ( به ترتیب37/1و 36/1) بود که با سایر تیمارها  اختلاف معنی‌داری داشت ) 05/0<(P. تاثیر تیمارهای آزمایشی بر درصد لاشه معنی‌دار بود ) 05/0<  (P. بیشترین درصد لاشه مربوط به تیمار 5 (3/73 درصد) و کمترین درصد لاشه مربوط به تیمار 2 (4/68 درصد) بود ) 05/0<  (P.  غلظت مالون‌دی‌آلدئید در گوشت سینه و ران در گروه‌های دریافت کننده‌ سطوح مختلف پپتید و تیمار 2 کمتر از تیمار شاهد بود. به طورکلی، نتایج این آزمایش نشان داد که استفاده از پپتیدهای زیست‌فعال استخراج شده از کنجاله تخم‌آفتابگردان، علاوه بر بهبود عملکرد، سبب افزایش کیفیت گوشت در مدت ذخیره‌سازی پس از کشتار از طریق کاهش اکسیداسیون چربی‌ها می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of extracted peptides from sunflower seed meal on performance, carcass characteristics, and antioxidant activity in broiler chickens

نویسندگان [English]

  • Hamid Ashkevar Ghorbani 1
  • Mansour Rezaei 2
  • Mohammad Kazemifard 2
1 Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

This experiment was conducted to investigate the effect of extracted peptides from sunflower seed meal on performance, carcass characteristics, and antioxidant activity in broiler chickens. In this experiment, 200 Ross 308 male broiler chicks were used in a completely randomized design with five treatments, four replicates, and 10 chicks per each replicate, for 26 days. Experimental treatments included: 1) Control diet without any additives, 2) Control diet + 300 mg vitamin E per kg diet, 3) Control diet + 250 mg sunflower seed meal peptides per kg diet, 4) Control diet + 500 mg sunflower seed meal peptides per kg diet, 5) Control diet + 1000 mg sunflower seed meal peptides per kg diet. There were significant differences between treatments for feed conversion ratio and body weight gain in grower (days 11-26)and whole periods(days 1-26) of the experiment (p<0.05). The most, and least body weight gain belonged to treatment 5, and control treatment. Treatment 5 showed  least feed conversion ratio in grower and whole period (1.37 and 1.36) respectively (p<0.05). The effect of experimental treatments on carcass percentage was significan and highest carcass percentage belonged to treatment 5 (73.31%), and the lowest was observed in treatment 2 (68.37%) (p<0.05). The malondialdehyde concentration in breast and thigh meat treatments  received different levels of peptides and also treatment 2 was lower than the control treatment. In conclusion, results of the present experiment, showed that use of sunflower seed meal peptides have antioxidant activity and can improve performance, increase carcass quality, and meat stability during storaging through decreasing fat oxidation.  

کلیدواژه‌ها [English]

  • Bioactive peptides
  • broiler
  • performance
  • sunflower seed meal

Extended Abstract

Introduction

    In recent years, attention to use of new feed additives such as, bioactive peptides derived from animal or plant origin in poultry diets to enhance performance and meat stability has been  increased. There is not published data about effect of using bioactive sunflower seed meal peptides on broiler performance and antioxidant activity. Thus the aim of the present experiment was to investigate the effect of sunflower seed meal peptide on performance, carcass characteristics, antioxidant activity and breast and thigh meat storage stability in broiler chickens.

 

Meterials and Methods

    In this experiment a completely randomized design with200 Ross 308 male broiler chicks , five treatments, four replicates and 10 chicks per replicate was used Experimental treatments included: 1) Control diet without any additives 2) Control diet + 300 mg vitamin E per kg diet 3) Control diet + 250 mg sunflower seed meal peptides per kg diet 4) Control diet + 500 mg sunflower seed meal peptides per kg diet 5) Control diet + 1000 mg sunflower seed meal peptides per kg diet. Feed intake, body weight were measured in starter (days 1 to 10), grower (days 11 to 26), and whole period of the experiment (days 1 to 26) and feed conversion ratio was also calculated in these periods. On 26 days of age, one bird with body weight, close to the pen average body weight was selected from each replicate, was slaughtered and breast, thigh, abdominal fat and organ weight such as heart, spleen, liver, and pancreas, were measured. Samples from breast and thigh meat were taken and kept at -20°C to measure serum antioxidant activity. Ferric reducing antioxidant power (FRAP) was determined. Data of this experiment was analysed using  GLM procedure in SAS sowftware.  Differences between treatments mean was determined by Duncan,s multiple range test at p<0.05.

 

Results

    The lowest and highest  feed conversion ratio in grower and whole periods were observed in treatment 5 control treatments respectively. The higher and lower body weight gain belonged to treatment 5 and control group, respectively. The effect of experimental treatments on carcass percentage was significant (P<0.05). Teatments 3 and 4 showed higher carcass and breast percentage, which was significantly differed from the other treatments (P<0.05). The higher percentage of abdominal fat was observed in control group and treatment 5. Lowere glucose concentration was observed in treatments 1 and 5. Treatment 5 had higher triglycerides concentration which was significantly differed from the other treatments. The least cholesterol concentration belonged to treatments 4 and 5 (P<0.05). The least glutamic oxaloacetic transaminase value (aspartate aminotransferase), [GOT (AST)] was observed in treatment 3. Higher , and the lowere concentrations of low-density lipoprotein (LDL) belonged to cotrol, treatments 2 and 5 respectively. Higher very low density lipoprotein (VLDL) concentration was observed in treatment 5 (P<0.05) and  higher malondialdehyde (MDA) concentration was observed in control group, and lowere of that  was seen in treatments 2, 4 and 5 (P<0.05) respectively. The higher ferric reducing antioxidant power value belonged to treatment 5 and the lowere of that  related to the control and treatment 3.

 

Conclusion

     The results of the present study showed that, peptides extracted from sunflower seed meal have antioxidant activity and can improve the performance, carcass qualities in   and also  meat storage stability. Therefore, with the economical consideration , use of  sunflower seed meal peptidescan up to 1000 mg per kg broiler diet in whole period (1 to 26 days) is recommended.

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علوم دامی ایران
دوره 55، شماره 4
دی 1403
صفحه 603-620

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  • تاریخ دریافت: 22 فروردین 1402
  • تاریخ بازنگری: 12 تیر 1402
  • تاریخ پذیرش: 23 تیر 1402

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