بررسی اثرات افزودن کروسین بر رقیق‌کننده مایع منی سگ در طی نگهداری در یخچال

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه مامایی و طیور، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران

2 گروه تولیدمثل دام های کوچک، دانشکده دامپزشکی، دانشگاه هانوفر، هانوفر، آلمان

چکیده

تلقیح مصنوعی با استفاده از منی سرد یکی از تکنیک‌های قابل‌دسترس در پرورش حیوانات اهلی است، اما فرایند سرد کردن به دلیل افزایش تنش اکسیداتیو سبب کاهش چشمگیری در کیفیت اسپرم می‌شود. هدف از مطالعه حاضر، بررسی اثرات کروسین بر نگهداری مایع منی سگ در دمای ۴ درجه سانتی‌گراد در طی دوره ۴۸ ساعته است. در این مطالعه، ۲۵ انزال از ۵ سگ نژاد تریر گرفته شد و در یک رقیق‌کننده بر پایه تریس رقیق شدند. سپس، آن‌ها به ۵ قسمت در گروه‌های شاهد، شاهد حلال (دی­متیل­سولفوکساید 5 درصد حلال کروسین) و تیمار شده با ۵/۰، ۱ و ۵/۱ میلی‌مولار کروسین تقسیم شدند. فراسنجه‌های اسپرم ازجمله، جنبایی کل و پیش‌رونده، شاخص‌های جنبایی و قابلیت زنده ماندن اسپرم‌ها به مدت ۴۸ ساعت ارزیابی شدند. نتایج به‌دست‌آمده نشان داد که جنبایی پیش‌رونده و کلی در طی ۴۸ ساعت ذخیره‌سازی به ترتیب در غلظت‌های ۵/۱، ۱ و ۵/۰ میلی‌مولار کروسین در مقایسه با گروه شاهد به‌طور معنی‌داری بالاتر بودند. همچنین بررسی شاخص‌های جنبایی به‌استثناء راستی مسیر طی شده  نشان دادند که افزودن ۵/۱ میلی‌مولار کروسین به‌طور معنی‌داری باعث بهبود شاخص‌ها نسبت به گروه شاهد می‌شود. بررسی قابلیت زنده‌ماندن اسپرم‌ها نشان داد که به‌طور معنی‌داری افزودن به ترتیب ۵/۱، ۱ و ۵/۰ میلی‌مولار کروسین باعث بهبود زنده‌مانی اسپرم‌ها می‌شود. درنتیجه، مطالعه حاضر نشان داد که افزودن کروسین به مایع منی سگ، می‌تواند فراسنجه‌های منی سگ را پس از ذخیره‌سازی به‌صورت مایع بهبود ببخشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Evaluation of the effects of crocin addition on canine semen dilution during refrigerated storage

نویسندگان [English]

  • Saeed Salehi 1
  • Ali Soleimanzadeh 1
  • Sandra Goericke-Pesch 2
  • Esmail Ayen 1
1 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2 Department of Small Animal Reproductive Medicine, Faculty of Veterinary Medicine, University of Hanover, Hanover, Germany
چکیده [English]

Artificial insemination is one of the most accessible techniques for raising domestic animals. It has been proven that refrigeration significantly reduces the quality of sperm. The aim of this study is to investigate the effects of crocin on the storage of dog semen at 4 ºC during a period of 48 hours. In this study, 25 ejaculates were collected from 5 terrier dogs and diluted in a Tris-based diluent. Then, they were divided into 5 parts in control, control dissolve (Dimethyl sulfoxide 5%, dissolve of crocin) and treated with 0.5, 1 and 1.5 mM crocin groups. Sperm parameters including evaluation of total motility, progressive motility, motility characteristics and sperm viability were evaluated for 48 hours. The obtained results showed that progressive motility and total motility during 48 hours of storage were significantly higher in 1.5, 1, and 0.5 mM crocin concentrations compared to the control group. Also, the examination of motility characteristics, except for STR, showed that the addition of 1.5 mM crocin significantly improved the characteristics compared to the control group. Investigating sperm viability showed that the addition of 1.5, 1 and 0.5 mM crocin significantly improves sperm viability. As a result, the present study determined that the addition of crocin to dog semen can improve the parameters of dog semen after liquid storage.

کلیدواژه‌ها [English]

  • Antioxidants
  • Crocin
  • dog sperm
  • Oxidative stress

Extended Abstract

Introduction

Artificial insemination is one of the most accessible techniques for raising domestic animals. It has been proven that refrigeration significantly reduces the quality of sperm. Various studies have shown that sperm cooling and freezing leads to increased oxidative stress and excessive reactive oxygen species production, and this process ultimately leads to membrane lipid peroxidation and sperm damage. In general, the introduction of sperm into a laboratory environment leads to lipid peroxidation and ultimately decreases DNA quality and integrity. During sperm storage, there are insufficient antioxidants to prevent free radical damage and lipid peroxidation in sperm. Sperm freezing leads to problems such as decreased sperm viability and motility after the freeze-thaw process. The aim of this study is to investigate the effects of crocin on the storage of dog semen at 4 ºC during a period of 48 hours.

 

Materials and Methods

In this study, 25 ejaculates were collected from 5 terrier dogs and diluted in a Tris-based diluent. Then, they were divided into 5 parts in control, control dissolve (Dimethyl sulfoxide 5%, dissolve of crocin) and treated with 0.5, 1 and 1.5 mM crocin groups. Sperm parameters including evaluation of total motility, progressive motility, motility characteristics and sperm viability were evaluated for 48 hours. The obtained results showed that progressive motility and total motility during 48 hours of storage were significantly higher in 1.5, 1, and 0.5 mM crocin concentrations compared to the control group. Also, the examination of motility characteristics, except for STR, showed that the addition of 1.5 mM crocin significantly improved the characteristics compared to the control group. Investigating sperm viability showed that the addition of 1.5, 1 and 0.5 mM crocin significantly improves sperm viability.

 

Discussion

This study is to investigate the effects of crocin on the storage of dog semen at 4 ºC during a period of 48 hours.  The present results showed that sperm samples containing crocin significantly improve the qualitative parameters of dog sperm after storage so that the fertility potential can be increased. The process of freezing-thawing and preserving sperm increases reducing sperm motility and viability and increasing the amount of free radicals. The before studies showed that the quality and integrity of the sperm plasma membrane is positively related to the rate of fertility. In the present study, it has been shown that the viability of sperm decreases during storage in the refrigerator. Also, the present study showed that adding crocin to dog semen increases sperm viability. As a result, the present study determined that the addition of crocin to dog semen can improve the parameters of dog semen after liquid storage. In this study, dose-dependent positive effects of crocin on quality parameters of dog sperm during refrigerated storage were shown. Considering the positive effects of crocin, the present study confirms the use of crocin in canine semen extenders. However, more research on fertility rates with crocin-containing sperms is needed to confirm its protective effects.

 

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علوم دامی ایران
دوره 54، شماره 3
مهر 1402
صفحه 303-315

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  • تاریخ دریافت: 19 مهر 1401
  • تاریخ بازنگری: 21 آذر 1401
  • تاریخ پذیرش: 07 دی 1401

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