مطالعه اثرات برون‌تنی آکوااستارت بر ترونت‌های ایکتیوفتیریوس مولتی فیلییس در ماهی قزل‌آلای رنگین‌کمان

نوع مقاله : مقاله پژوهشی

نویسندگان

• جلیل رحمتی طاقجه حسن ¹
• محمد یخچالی ²
• علی نکوئی فرد ³

¹ گروه پاتوبیولوژی، بخش انگل‌شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه،ایران

² گروه پاتوبیولوژی،‌‌بخشانگل‌شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه،ایران.

³ مرکز تحقیقات آرتمیای کشور، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران

چکیده

کلیدواژه‌ها

• آکوااستارت
• ایکتیوفتیریوس‌مولتی ‌فیلییس
• ترونت
• قزل‌آلای رنگین‌کمان

موضوعات

• مدیریت و بهداشت دام

عنوان مقاله [English]

In-vitro effects of Aquastart on theronts of Ichthyophthirius multifiliis in rainbow trout (Oncorhynchus mykiss)

نویسندگان [English]

• Jalil Rahmati Taghjeh Hassan ¹
• Mohammad Yakhchali ²
• Ali Nekuie fard ³

کلیدواژه‌ها [English]

• Aquastart
• Ichthyophthirius multifiliis
• Rainbow trout
• Theront

Extended Abstract

Introduction

Rainbow trout (Oncorhynchus mykiss) is one of the economically and nutritionally important famed species of cold-water fish in Iran. Ichthyophthirius multifiliis is a parasitic protozoan infection; causes economic losses in to both aquaculture and ornamental fish industries. Rainbow trout is a sensitive fish to white spot disease. Ichthyophterius multifiliis is a ciliate protozoan with global distribution and low host specificity. It has the potential to destroy all fish in a farm. There are different levels of resistance among different hosts. So far, no vaccine and resistance has been reported in farmed fish against I. multifiliis infection. In earlier years, green malachite was applied as an effective compound against I. multifiliis, but it has been prohibited in many countries because of its carcinogenic and teratogenic effects in humans. In addition, other chemical compounds like copper sulfate, sodium chloride, potassium permanganate, and formalin, have some environmental issues such as aquatic ecosystems changes with lower efficiency and higher cost. Thus, the seeking for effective and environmental agents and management procedures to prevent I. multifiliis infection is essential. Aquastart is as an effective antimicrobial agent against other fish pathogens in aquaculture. Additionally, production time and degradation of Aquastart were introduced it as a suitable disinfectants in aquaculture. The present study aimed to investigate in-vitro effect of Aquastart (antiseptic solution) on I. multifiliis isolated from rainbow trout.

Materials and Methods

 From a rainbow trout fish farm, a total number of 20 infected rainbow trout fish with body weight average 20±4 gr and length 14±2 cm and I. multifiliis trophont were trapped from a rainbow trout breeding farm suffering from ICH disease. Then, 80 uninfected fingerling rainbow trout were gotten exposed to each other in three stages. To isolate trophonts, their skin and gills were gently scraped in a petri dish containing chlorine-free water and the scarps transferred to a petri dish containing chlorine-free water. After 2 hours, the tomonts were attached to the bottom of the Petri dish, washed three times with chlorine-free water, and incubated for 24 hours at 23°C. Afterwards to release and counting the theronts, 2 µL of parasite containing suspension were placed on the slide and 2 µL of 1% formalin were added. Concentrations of 200, 100, 50, 20, 10 and 5 mg/L were prepared from the stock concentration of Aquastart (400 mg/L) and 300 theronts were added. The lethality level of aquastart was evaluated with presence of control groups, i.e. malachite green (0.05 mg/ µL) and water without Aquastart (50µL).

Results and discussion

       The minimum time of occurrence of the lethal effect of Aquastart at concentration of 200 mg/L was 0.67 ± 0.038 and the maximum time of its effect at a concentration of 100 mg/liter was 150 ± 1.2 seconds. The minimum and maximum lethal effect of Aquastart were at 0.67 ± 0.038 (200 mg/L) and 150 ± 1.2 (5 mg/L), respectively. The duration of lethal effect of Aquastart had significant association with concentration.

Conclusion

       From the results of this study, it was concluded that Aquastart had toxic effect on theronts of I. multifiliis and different concentrations of Aquastart at different time durations eliminated them.