مقایسه تاثیرتزریق درون تخم‌مرغی L و DL متیونین بر رشد جنین، درصد جوجهدرآوری و عملکرد جوجه های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران.

3 گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

پژوهش کنونی با هدف بررسی تاثیر تزریق درون تخم‌مرغی سطوح مختلف اسیدآمینه L و DL-متیونین بر درصد جوجه‌درآوری، عملکرد و فراسنجه‌‌های بیوشیمیایی خون جوجه‌‌ها تا سن هفت روزگی انجام شد. به این منظور در روز 16 جوجه‌‌کشی، 800 عدد تخم‌مرغ نطفه‌دار سویه راس 308 به‌طور تصادفی بین 8 تیمار (4 تکرار) به‌‌صورت 3 سطح L-متیونین (10، 20 و 30 میلی‌گرم)، 3 سطح DL-متیـونین (10، 20 و 30 میلی‌گرم) و 2 تیمار شاهد، شامل شاهد منفی (بدون تزریق) و مثبت (تزریق 200 میکرولیتر سالین) تقسیم شدند. تزریق تیمارها به‌طور معنی‌داری درصد جوجه‌درآوری را کاهش داد (05/0(P<، اما اثری بر وزن جوجه‌های تفریخ شده، نسبت وزن جوجه‌ به وزن تخم‌مرغ و مصرف خوراک در هفته نخست دوره پرورش نداشت. بیشترین افزایش وزن روزانه (3/27 گرم) و بهترین ضریب تبدیل خوراک مصرفی (62/0) در تیمار 30 میلی‌گرم L-متیـونین دیده شد. نسبت وزن لاشه به وزن زنده (5/50) در تیمار 30 میلی‌گرم L-متیونین به طور معنی‌داری (05/0P<) افزایش یافت. سطح تری‌گلیسرید خون جوجه‌ها در 7 روزگی در همه‌‌ی تیمارها نسبت به تیمار شاهد بدون‌‌تزریق به طور معنی‌‌داری کاهش یافت. به‌‌طور‌‌کلی نتیجه‌‌ی این پژوهش نشان داد که تزریق 30 میلی‌گرم L-متیونین در مقایسه با DL-متیونین می تواند منجر به افزایش عملکرد و بهبود نسبت لاشه در 7 روزگی شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Comparison of the effect of in ovo injection of L and DL methionine on embryonic growth, hatchability percentage and performance of broiler chickens

نویسندگان [English]

  • Morteza Gholami 1
  • Zarbakht Ansari Pirsaraei 2
  • Hamid Deldar 2
  • Mohammad Kazemi-Fard 3
1 Animal Science Department, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2 Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
3 Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
چکیده [English]

Methionine is the first limiting amino acid in the poultry diet, and it plays essential roles in energy production and protein synthesis, increasing the growth performance of broiler chickens. This trial was conducted to compare the effect of L- and DL-Methionine on hatchability, productive performance and biochemistry of some blood parameters of broiler chicken from 1 to 7 day of age. On days 16 of incubation, 800 fertile egg (Ross 308) were randomly divided between 8 treatments (and 4 replicates) including 3 levels of L-Methionine (10, 20 and 30 mg), 3 levels of DL-Methionine (10, 20 and 30 mg) and two control treatments including negative (no injection) and positive (injection of 200 microliters of saline) control. Results showed that all treatments significantly (p<0.05) decreased the hatchability, without no effect on chick weight, chick to egg weight ratio and feed intake from 1 to 7 days of age. Birds received 30 mg L-Methionine showed the best BWG (27.3 g) and FCR (0.62). Likewise, 30 mg L-Methionine increased the carcass to live weight ratio (50.5) significantly (p<0.04). The applied treatments compare to the negative control group significantly (P<0.04) decreased triglyceride content of serum in the chickens. In general, the results of this research showed that injection of 30 mg of L-methionine compared to DL-methionine can lead to increased performance and improved carcass ratio in 7 days.

کلیدواژه‌ها [English]

  • Amino acid
  • Broiler
  • DL- Methionine
  • in ovo injection
  • L-Methionine

Extended Abstract

Introduction and Objective: Methionine is the first limiting amino acid in the poultry diet, and it plays essential roles in energy production and protein synthesis, increasing the growth performance of broiler chickens. The current study was aimed to compare the effect of L- and DL-Methionine on hatchability, productive performance and biochemistry of some blood parameters of broiler chicken from 1 to 7 day of age.

 

Materials and methods

    On days 16 of incubation, 800 fertile egg (Ross 308) were randomly divided between 8 treatments (and 4 replicates) including 3 levels of L-Methionine (10, 20 and 30 mg), 3 levels of DL-Methionine (10, 20 and 30 mg) and two control treatments including negative (no injection) and positive (injection of 200 microliters of saline) control. After 21 days, the hatchability of eggs was determined, and the hatchlings weighed were also evaluated. chicks were transferred in farm of agriculture and Natural Resources University of Sari (Iran). Feed intake was measured during experiment and mortality was recorded daily. After the first week chicks were weighed and feed: gain was measured. Then, Blood was collected and centrifuged to separate the plasma. Blood parameters were measured by the spectrophotometric method according to the instructions of the manufacturer of the kits.

 

Results

    Results showed that all treatments significantly (p<0.05) decreased the hatchability, without no effect on chick weight, chick to egg weight ratio and feed intake from 1 to 7 days of age. Birds received 30 mg L-Methionine showed the best BWG (27.3 g) and FCR (0.62). Likewise, 30 mg L-Methionine increased the carcass to live weight ratio (50.5) significantly (p<0.04). The applied treatments compare to the negative control group significantly (P<0.04) decreased triglyceride content of serum in the chickens.

 

Conclusion

 In general, the results of this research showed that injection of 30 mg of L-methionine compared to DL-methionine can lead to increased performance and improved carcass ratio in 7 days.

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