تأثیر افزودن مخلوطی از اسانس‌های نعناع فلفلی، آویشن و رزماری به جیره بر عملکرد رشد، ‏فراسنجه‌های تخمیر شکمبه‌ و متابولیت‌های خون بره‌های پرواری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، دانشگاه رازی، کرمانشاه، ایران

2 دانشیار، گروه علوم دامی، دانشکده علوم و مهندسی کشاورزی، دانشگاه رازی، کرمانشاه، ایران

3 استاد، گروه علوم دامی ، دانشکده علوم و مهندسی کشاورزی، دانشگاه رازی، کرمانشاه، ایران

چکیده

این آزمایش برای ارزیابی اثر افزودن مخلوط اسانس­های نعناع فلفلی، آویشن و رزماری به جیره به ترتیب با نسبت 3:1:2 بر عملکرد رشد، فراسنجه­های شکمبه و خون بره­های پرواری انجام شد. بیست و یک رأس بره نر مهربان با میانگین وزن 80/29 کیلوگرم به‌طور تصادفی با یکی از سه جیره 1) شاهد، 2) جیره پایه + 25/1 میلی­لیتر مخلوط اسانس و 3) جیره پایه + 5/2 میلی­لیتر مخلوط اسانس تغذیه شدند. افزایش وزن روزانه در گروه‌های دریافت‌کننده مخلوط اسانس‌ها بیشتر از گروه شاهد بود، هرچند این افزایش بیشتر ناشی از اثر متقابل تیمار در زمان بود. میزان مصرف خوراک و ضریب تبدیل خوراک نسبت به گروه شاهد تغییری نکرد. غلظت کل اسیدهای چرب فرّار و استات در گروه‌های دریافت‌کننده مخلوط اسانس نسبت به گروه شاهد کاهش یافت(05/0>P)، در حالی که غلظت پروپیونات در 5/2 میلی‌لیتر اسانس و غلظت ایزووالرات در غلظت‌های مختلف مخلوط اسانس نسبت به گروه شاهد افزایش یافت(05/0>P). تعداد پروتوزوآ، pH و نیتروژن آمونیاکی شکمبه تفاوت معنی‌دار نداشت. غلظت گلوکز در سطح بالای مخلوط اسانس‌ها افزایش یافت (05/0>P). غلظت‌های اوره، کلسترول، تری‌گلیسرید، کراتینین، پروتئین کل و آلبومین تغییری نکرد. تعداد سلول‌های قرمز، هموگلوبین، هماتوکریت، تعداد و درصد سلول‌های سفید شامل لنفوسیت و ائوزینوفیل تحت تأثیر تیمارها قرار نگرفت ولی درصد مونوسیت‌ها در سطح پایین مخلوط اسانس‌ها کاهش یافت. نتایج این آزمایش نشان داد که افزودن مخلوط اسانس­ها به جیره تأثیر معنی‌داری بر فراسنجه‌های عملکرد و سیستم ایمنی بره‌ها نداشت، هر چند سبب کاهش غلظت کل اسیدهای چرب فرّار، استات و افزایش غلظت پروپیونات در شکمبه شد. 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of adding a mixture of peppermint, thyme and rosemary essential oils to ‎diet on growth performance, rumen fermentation parameters and blood metabolites ‎of fattening lambs

نویسندگان [English]

  • Marzieh Rahmati Zaed 1
  • Fardin Hozhabri 2
  • Farokh Kafilzadeh 3
1 Ph.D. Candidate, Department of Animal Science, Faculty of Science and Agricultural ‎Engineering, Razi University, Kermanshah, Iran
2 Associate Professor, Department of Animal Science, Faculty of Science and Agricultural Engineering, Razi University, ‎Kermanshah, Iran
3 Professor, Department of Animal Science, Faculty of Science and Agricultural Engineering, Razi University, Kermanshah, Iran
چکیده [English]

This study was performed to evaluate the effect of adding a mixture of peppermint, thyme and rosemary essential oils (EOs) in a ratio of (3:1:2), respectively, to the diet, on growth performance, rumen and blood parameters of fattening lambs. Twenty-one Mehraban lambs with an average weight of 29.80 kg were randomly allocated to one of three diets: 1) control, 2) base diet+1.25ml of EOs and 3) base diet+2.5ml of EOs. Daily weight gain was greater in the groups receiving the EOs than in the control, although this increase was mainly due to the interactions of treatment over time. Feed consumption and feed conversion ratio compared to the control did not altered. The total concentrations of VFAs and acetate in the groups receiving EOs decreased (P<0.05), while the concentration of propionate increased (P<0.05) at the level of 2.5ml of EOs and isovalerate increased (P<0.05) in both levels of EOs compared to the control. Total population of protozoa, rumen pH and ammonia nitrogen concentration were not affected by treatments. Glucose concentration increased at high level of EOs (P<0.05). Concentrations of urea, cholesterol, triglycerides, creatinine, total protein and albumin did not altered. The number of red blood cells, hemoglobin, hematocrit, number and percentage of white cells including lymphocytes and eosinophils were not affected by treatments but the percentage of monocytes decreased (P<0.05) at low level of the EOs. The results of this experiment showed that the addition of mixture of EOs to the diet did not have a significant effect on performance parameters and immune system of lambs, although it reduced the total concentration of VFA and acetate and increased the concentration of propionate.

کلیدواژه‌ها [English]

  • Blood cells and metabolites
  • herbal essential oil
  • Mehraban lambs
  • rumen fermentation

مراجع

  1. Abd El Tawab, A. M., Kholif, A. E., Khattab, M. S. A., Shaaban, M. M., Hadhoud, F. I., Mostafa, M. M. M. & Olafadehan O. A. (2020). Feed utilization and lactational performance of Barki sheep fed diets containing thyme or celery. Small Ruminant Research, 192, 1-9. Academy Press. Washington. DC. 384 pages.
  2. Agarwal, N., Shekhar, Ch., Kumar, R., Chaudhary, L. C. & Kamra, D. N. (2009). Effect of peppermint (Mentha piperita) oil on in vitro methanogenesis and fermentation of feed with buffalo rumen liquor. Animal Feed Science and Technology, 148, 321-327.
  3. Ahmed, M. G., El-Zarkouny, S. Z., El-Shazly, K. A. & Sallam, S. M. A. (2014). Impact of essential oils blend on methane emission, rumen fermentation characteristics and nutrient digestibility in barki sheep. Journal of Agricultural Science, 6(7), 144-156.
  4. Akhavan Gigloo, T., Hozhabri, F. & Souri, M. (2019). Two different dietary antioxidants: Blood immune parameters and performance in Sanjabi suckling lambs. Journal of Livestock Production, 21(1), 23- 35. (In Farsi)
  5. Akram, M. Z., Asghar, M. U. & Jalal, H. (2021). Essential oils as alternatives to chemical feed additives for maximizing livestock production. Journal of the Hellenic Veterinary Medical Society, 72(1), 2595-2610.
  6. Al-Desouky, A., Abdelaziz, Sh. & Ratib, M. O. (2019). Effect of thyme, ginger and boldenone as growth promoters on some biochemical blood parameters in white male new zealand rabbits. Benha Veterinary Medical Journal, 37(2), 6-13.
  7. Allam, S. & El-Elaime, R. R. (2020). Impact of garlic, lemongrass, peepermint and rosemary as feed additives on performance of growing barki lambs. Egyptian Journal of Nutrition and Feeds, 23(3), 359-367.
  8. (1990). Official Methods of Analysis, 17th edition. Association of official analytical chemists, Arlington, VA.
  9. Baraz, H., Jahani‐Azizabadi, H. & Azizi, O. (2021). Effects of thyme essential oil and disodium fumarate alone or in combination on performance, blood metabolites, ruminal fermentation and microbial communities in holstein dairy cows. Iranian Journal of Applied Animal Science, 11(2), 261-270.
  10. Benchar, C., Calsamiglia, S., Chaves, A. V., Fraser, G. R., Colom-batto, D., Mcallister, T.A. & Beauchemin, K. A. (2008). A review of plant-derived essential in ruminant nutrition and production. Animal Feed Scienc and Technology, 145, 209-228.
  11. Bhat, A. R., Ganai, A. M., Ishfaq, A., Masood, D., Sheikh, G. G & Beigh, Y. A. (2017). Effects of mentha piperita on in vitro fermentation and digestibility of complete rations for small ruminants. Indian Journal of Animal Nutrition, 34 (2), 173-177.
  12. Broderick, G.A. & Kang, J. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminanl fluid and in vitro Journal of Diary Science, 63, 64-75.
  13. Craig, W. J. (1999). Health-promoting properties of common herbs. American Journal of Clinical Nutrition, 70, 491-499.
  14. Dehority, B.A. (2003). Rumen Microbiology. Nottingham University Press, Nottingham. UK.
  15. Douiri, L.F., Bougdad, A., Assobhei, O. & Moumni, M. (2013). Chemical composition and biological activity of essential of alulium sativum agalnst callosobruchus maculatus. Journal of Environmental Science, Toxicology and Food Technology, 3(1), 30- 36.
  16. Ebrahimi, M. A., SobhaniRad, S. & Bayat, A. R. (2018). Effects of ajwain (Trachyspermum ammi) and thyme (Thymus vulgaris) oils on nutrients digestibility, blood parameters and growth performance of brown swiss neonatal calves. Iranian Journal of Applied Animal Science, 8(3), 387-395.
  17. El-Essawy, A. M., Abdou, A. R., Khattab, I. M. & Abdel-Wahed A. M. (2019). Effect of addition of anise, clove and thyme essential oils on barki lambs’ performance, digestibility, rumen fermentation, carcass characteristics and intramuscular fatty acids. Egyptian Journal. Nutrition and Feeds, 22 (3), 465- 477.
  18. El-Essawy, A. M., Aneleb, U. Y., Abdel-Waheda, A. M., Ahlam, A. M., Abdoua, R. & Khattab, I. M. (2020). Effects of anise, clove and thyme essential oils supplementation on rumen fermentation, blood metabolites, milk yield and milk composition in lactating goats. Animal Feed Science and Technology, P: 1-40.
  19. Fazlara, A., Pourmahdi, M., Zarei, M. & Karimi, T. (2017). Effect of edible chitosan-rosemary coating on quality and shelf life of refrigerated chicken fillets. Iranian Veterinary Journal, 13(1), 78-90.
  20. Ibrahim, O.A.E.H., Mohamed, A.G. & Bahgaat, W.K. (2019). Natural peppermint-flavored cheese. Acta Scientiarum Polonorum Technologia Alimentaria, 18,75-85.
  21. Kaki, S., Moeini, M. M. & Hozhabri, F. (2020). Effects of mixed crushed caraway (Carum carvi) with chromium-methionine or zinc-methionine supplementations on serum components and physiological responses of lambs subjected to transportation stress. Small Ruminant Research, 183.
  22. Kaki, S., Moeini, M. M., Hozhabri, F. & Nikousefat, Z. (2018). The use of crushed caraway (Carum carvi) and black seed (Nigella sativa) additives on growth performance, antioxidant status, serum components and Physiological responses of sanjabi lambs. Iranian Journal of Applied Animal Science, 8(3), 439-444.
  23. Khamisabadi, H. & Fazaeli, H. (2021). Effect of thymus vulgaris or peppermint on lactating sanjabi ewe performance, milk composition, lamb growing and relevant blood metabolites. Journal of Medicinal Plants and By-products, 1, 95-101.
  24. Khamisabadi, H. (2016). The Effect of Adding Peppermint or Thyme to the Diet on Gastrointestinal Development, Immune System, Production Performance and Meat Quality in Fattening Lambs.D. Thesis. Faculty of Agriculture Razi University, Kermanshah. (In Farsi).
  25. Khamisabadi, H., Kafilzadeh, F. & Charaien, B. (2016). Effect of thyme (Thymus vulgaris) or peppermint (Mentha piperita) on performance, digestibility and blood metabolites of fattening Sanjabi lambs. Biharean Biologist, 10 (2), 118-122.
  26. Khateri, N., Azizi, O. & Jahani-Azizabadi, H. (2017). Effects of a specific blend of essential oils on apparent nutrient digestion, rumen fermentation and rumen microbial populations in sheep fed a 50:50 alfalfa hay: concentrate diet. Asian-Australas Journal of Animal Science, 30(3), 370-378.
  27. Kholif, A. E., Matloupa, O. H., Morsya, T. A., Abdo, M. M., Abu Elellab, A., Anelec, U. Y. & Swanson, K. C. (2017). Rosemary and lemongrass herbs as phytogenic feed additives to improve efficient feed utilization, manipulate rumen fermentation and elevate milk production of Damascus goats. Livestock Science, 204, 39-46.
  28. McEwan, N. R., Graham, R. C., Wallace, R. J., Losa, R., Williams, P. & Newbold, C. J. (2002). Effect of essential oils on ammonia production by rumen microbes. Reproduction Nutrition Development, 42 (Suppl. 1): S65 (abstract).
  29. Mirzaei Cheshmehgachi, S., Moeini, M. M., Hozhabri, F. & Nooryan Soroor, M. E. (2017). Effect of essential oils of zataria multiflora, eucalyptus globolus and their combination on fermentation parameters using merghoz goat rumen liquor. Iranian Journal of Applied Animal Science, 7(1), 53-59.
  30. Mirzaei, Z., Hozhabri, F. & Alipour, D. (2016). Thymus kotschyanus essential oil components and their effects on in vitro rumen fermentation, protozoal population and acidosis parameters. Iranian Journal of Applied Animal Science, 6(1), 77-85.
  31. Mohamadi, R., Rahchamani, R., Ghanbari, F. & Farivar, F. (2017). Peppermint and pennyroyal essential oil effect on performance, rumen microbial population and some blood parameters of sheep. Iranian Journal of Veterinary Medicine, 11(1), 75-84.
  32. Monteschio, J. O., Souza, K. A., Vital, A. C. P., Guerrero, A., Valero, M. V., Kempinski, E. M. B. C. & Prado, I. N. (2017). Clove and rosemary essential oils and encapsuled active principles (eugenol, thymol and vanillin blend) on meat quality of feedlot-finished heifers. Meat Science, 130, 50-57.
  33. Morshedy, S. A., Zweil, H. S., Zahran, S. M., Ahmed, M. H. & El-Mabrok, B. M. (2019). Growth performance, carcass traits, immune response and antioxidant status of growing rabbitts supplemented with peppermint and basil essential oils. Egyptian Poultry Science Journal, 39, 61-79.
  34. National Research Council. (2007). Nutrient Requirements of Small Ruminants. The National Academy Press. Washington. DC. 384 pages.
  35. Nieto, G., Diaz, P., Banon, S. & Dolores Garrido, M. (2010). Effect on lamb meat quality of including thyme (Thymus zygis gracilis) leaves in ewes’ diet. Meat Science,85, 82-88.
  36. Ortuno, J., Serrano, R., Jose Jordán, M. & Bañón, S. (2016). Relationship between antioxidant status and oxidative stability in lamb meat reinforced with dietary rosemary diterpenes. Food Chemistry, 190, 1056-1063.
  37. Osman, N. N., Alsharari, M. A. & Alsufiani, H. M. (2020). Peppermint (Mentha piperita) and thyme (Thymus vulgaris) attenuate the immune and inflammatory disorders in rats consumed repeatedly heated palm oil. Inter Journal Pharmaceutical Phytopharmacological Research (IJPPR), 10, 59-66.
  38. Puvaca, N., Lika, E., Cocoli, S., Shtylla Kika, T., Bursic, V., Vukovic, G., Tomas Simin, M., Petrovic, A. & Cara, M. (2020). Use of tea tree essential oil (Melaleuca alternifolia) in laying hen’s nutrition on performance and egg fatty acid profile as a promising sustainable organic agricultural tool. Sustainability, 12, 3420.
  39. Ribeiro, A. D. B., Ferraz Junior, M. V. C., Polizel1, D. M., Miszura1, A.A., Barroso1, J.P.R., Cunha, A.R., Souza, T.T., Ferreira, E.M., Susin, I. & Pires, A.V. (2020). Effect of thyme essential oil on rumen parameters, nutrient digestibility, and nitrogen balance in wethers fed high concentrate diets. Veterinary and Animal Science, 72 (2), 573-580.
  40. Rivaroli, D. C., do Prado, R. M., Ornaghi, M. G., Mottin, C., Ramos, T. R., Barrado, A. G., Jorge, A. M. & do Prado, I. N. (2017). Essential oils in the diet of crossbred (½ angus ½ nellore) bulls finished in feedlot on animal performance, feed efficiency and carcass characteristics. Journal of Agricultural Science, 9(10), 205-212.
  41. Saha, S., Mukhopadhyay, M., Ghosh, P. & Nath, D. (2012). Effect of methanolic leaf extract of Ocimum basilicum L. on benzene-induced hematotoxicity in mice. Evidence-Based Complementary and Alternative Medicine, 7 pp.
  42. Sahraei, M., Pirmohammadi R. & Payvastegan S. (2014). The effect of rosemary (Rosmarinus officinalis) essential oil on digestibility, ruminal fermentation and blood metabolites of Ghezel sheep fed barley-based diets. Spanish Journal of Agricultural Research,12(2), 448-454.
  43. SAS Institute (computer software). (2003). Users Guide: Statistics, version 9.2. Cary, NC, USA: SAS Institute, Inc.
  44. Savoini, G., Cattaneo, D., Paratte, R., Varisco. G., Bronzo, V., Moroni, P. & Pisoni, G. (2003). Dietary rosemary extract in dairy goats organically managed: effects on immune response, mammary infections and milk quality. Italian Journal of Animal Science 2, 548-550.
  45. Seirafy, H. & Sobhanirad, S. (2017). Effects of oregano (Origanum vulgare) and thyme (Thymus vulgaris) oils on growth performance and blood parameters in Holstein suckling calves. Iranian Journal of Applied Animal Science, 7(4), 585-593.
  46. Smeti, S., Hajji, H., Mekki, I., Mahouachi, M. & Atti, N. (2018). Effects of dose and administration form of rosemary essential oils on meat quality and fatty acid profile of lamb. Small Ruminant Research, S0921-4488(17)30279-1.
  47. Vakili, A. R., Khorrami, B., Danesh Mesgaran, M. & Parand, E. (2013). The effects of thyme and cinnamon essential oils on performance, rumen fermentation and blood metabolites in holstein calves consuming high concentrate diet. Asian Australian Journal of Animal Science, 26, 935-944.
  48. Walsh, S. E., Maillard, J. Y., Russell, A. D., Catrenich, C. E., Charbonneau, D. L. & Bartolo, R. G. (2003). Activity and mechanisms of action of selected biocidal agents on gram-positive and -negative bacteria. Journal of Applied Microbiology, 94, 240-247.
علوم دامی ایران
دوره 53، شماره 4
اسفند 1401
صفحه 273-285

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سابقه مقاله

  • تاریخ دریافت: 21 اسفند 1400
  • تاریخ بازنگری: 21 خرداد 1401
  • تاریخ پذیرش: 21 خرداد 1401

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