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اثر دمای‎ ‎منی رقیق‌شده و غلظت اسپرم بر نرخ نفوذ اسپرم، باروری و جوجه‌درآوری‎ ‎مرغ‌های مادر ‏گوشتی مسن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 پژوهش‌گر شرکت سهامی مزرعه نمونه (خاص)، سازمان اتکا

2 دانش‌آموخته دکتری رشته فیزیولوژی دام، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

هدف از این پژوهش بهینه‌سازی تلقیح مصنوعی در مرغ­های مادر گوشتی مسن در قالب دو آزمایش بود. در آزمایش نخست، اثر دو دمای متفاوت منی رقیق‌شده (5 و 25 درجه سلسیوس) خروس­ هوبارد (40 خروس، سن 58 هفته) بر باروری، جوجه درآوری و نرخ نفوذ اسپرم (SP) به لایه فراویتلین مرغ­ هوبارد (180 مرغ، سن 58 هفته) بررسی شد. در آزمایش دو، سه غلظت متفاوت اسپرم (100 (C100)، 200 (C200) و 400 (C400) میلیون اسپرم در 25/0 میلی‌لیتر (به‌ازای هر مرغ)، از خروس­های هوبارد (40 خروس، سن 62 هفته) بر باروری، جوجه درآوری و SP در 270 مرغ بررسی شد. در آزمایش نخست، یافته­ها نشان دادند دمای 5 درجه سلسیوس منی رقیق‌شده نسبت به دمای 25 درجه سلسیوس سبب افزایش درصد جوجه­درآوری کل، جوجه­درآوری تخم­مرغ­های بارور و SP و کاهش مرگ و میر جنینی اولیه شد. یافته­های آزمایش دو نشان دادند، بیشترین درصد باروری و SP در تیمار C400 گزارش شد. همچنین در این آزمایش بیشترین جوجه­درآوری کل و جوجه­درآوری تخم­مرغ­های بارور و کمترین میزان مرگ و میر جنینی اولیه در تیمارهای C200 و C400 گزارش شد. نسبت هزینه به فایده برای تیمارهای C200 و C400 به‌ترتیب تقریباً برابر است با 9/2 و 4/1 محاسبه شد. به طور کلی یافته­های این پژوهش نشان دادند که برای بهینه‌سازی تلقیح مصنوعی در مرغ­های مسن (با توجه به نسبت هزینه به فایده و جوجه­درآوری) می­توان از غلظت تلقیحی 200 تا 400 میلیون در 25/0 میلی‌لیتر به‌ازای هر مرغ در دمای 5 درجه سلسیوس استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of diluted semen temperature and sperm concentration on sperm penetration ‎rate, fertility and hatchability of aged broiler breeder hens

نویسندگان [English]

  • Hossein Sharideh 1 2
  • Mojtaba Zaghari 3
  • Seyed Jafar Mirbehbahani 1
1 Researcher of ETKA Organization ‎ |Ph.D. Graduate in Animal Physiology, Department of Animal Science, College of Agriculture and Natural ‎Resources, University of Tehran, Karaj, Iran
2 Researcher of ETKA Organization ‎ |Ph.D. Graduate in Animal Physiology, Department of Animal Science, College of Agriculture and Natural ‎Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The current study aimed to optimize artificial insemination in aged broiler breeder hens in two experiments. In the first experiment, the effect of (two) different diluted semen temperatures (5 and 25 °C) of Hubbard rooster (40 roosters, 58 weeks of age) on fertility, hatchability and sperm penetration (SP) rate in the perivitelline layer of Hubbard hen (180 hens) were investigated. In the second experiment, three (different) sperm concentrations (100 (C100), 200 (C200), and 400 (C400) million sperm in 0.25 mL per hen) of Hubbard roosters (40 roosters, 62 weeks of age) on fertility, hatchability and SP rate of Hubbard broiler breeder hens (270 hens) were explored. In the first experiment, the results showed that the temperature of 5 °C of diluted semen compared to the 25 °C, increased percentage of hatchability of set eggs, hatchability of fertile eggs, and SP and decreased early embryonic mortality. The results of the second experiment showed the highest percentage of fertility and SP rate were observed at treatment C400. Also, in this experiment that highest percentage of hatchability of set eggs and hatchability of fertile eggs and lowest early embryonic mortality were observed at treatment C400. Return on investment (ROI) of the treatments C200 and C400 was approximately 2.9 and 1.4, respectively. In overall, the results of this study showed that (in attention to ROI and hatchability) to optimize artificial insemination of aged broiler breeder hens we can use a sperm concentration of 200 to 400 million in 0.25 mL per hens at 5 °C.

کلیدواژه‌ها [English]

  • fertility
  • Extender temperature
  • Rooster
  • hatchability
  • Sperm penetration‎
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