This study was conducted to investigate the application of different levels of peeled and washed quinoa seeds on growth performance, immune response, nutrient digestibility and intestinal morphology in broilers. A total 280 Ross 308, one day old chicks were used in a completely randomized design with seven treatments, four replicates and 10 chicks per replicate. Experimental groups consisted of seven groups; fed 4, 8 and 12% peeled quinoa, or 4, 8 and 12% washed quinoa, and one control group. The results showed that using 4% peeled quinoa significantly increased the body weight and the feed intake. Broiler fed 4% peeled quinoa had lower FCR compare to the control (P<0.05). The using of 4% peeled quinoa increased the relative weight of carcass components including the breast and thigh (P<0.05) but decreased abdominal fat compared to the control group (P<0.05). The relative weight of bursa was higher in chicks fed different levels of quinoa (P<0.05). At 42 days of age the quinoa had no effect on the titer of immunoglobulin M and G index but increased total anti-SRBC index in broilers fed different levels of processed quinoa, compared to control (P<0.05). Quinoa reduced the concentration of cholesterol and LDL in broiler chicks (P<0.05). The processed quinoa at 4% increased the villus height and crypt depth (P <0.05), but did not affect the villus width of jejunum of broilers. It is concluded that using peeled and washed quinoa at 4% improved growth performance of broiler chickens. However, up to 12% washed or peeled quinoa had no negative effect on growth performance, and may decrease abdominal fat and blood lipid and may increase immune response of broiler chickens.