نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 محقق، دانشگاه فلوریدا، آمریکا ‏

چکیده

در پرورش گاوشیری هدف اصلی افزایش سود است. افزایش بیماری ورم‌پستان از مشکلات اصلی پرورش گاوشیری با کاهش طول‌عمر تولیدی و افزایش هزینه‌ها، سبب تحمیل زیان‌های اقتصادی جدی به این صنعت شده‌است. این مطالعه باهدف بررسی معماری ژنتیکی و شناسایی نواحی ژنومی مرتبط با صفات تولیدشیر و تعداد سلول‌های بدنی به‌عنوان یکی از شاخص‌های ارزیابی کیفیت شیر و معیاری غیرمستقیم از بیماری ورم‌پستان، انجام شده‌است. برای بررسی ارتباط و شناسایی پنجره‌های ژنومی اصلی، از روش مطالعه پیوستگی سراسری ژنوم یک‌مرحله‌ای با داده‌های ژنومی 1938 راس گاو نر استفاده شد. نتایج براساس واریانس ژنتیکی افزایشی پنجره‌‌های 5/1 مگابازی از SNP‌‌های مجاور ارائه شده است. پنجره‌هایی که بیش از 1% واریانس را کنترل می‌کنند به‌عنوان نواحی ژنومی اصلی و جهت یافتن ژن‌های کاندیدا استفاده شدند. تعداد 11 پنجره ژنومی اصلی روی 9 کروموزوم اتوزوم، حاوی 94 ژن کاندیدا حدود 20% واریانس ژنتیکی معیار سلول‌های بدنی را توصیف می‌کردند. بیشترین واریانس مربوط به پنجره‌ای روی کروموزوم 14 (85/3%) بود. تعداد 6 پنجره اصلی روی 6 کروموزوم شامل 89 ژن کاندیدا، مرتبط با تولیدشیر بودند. این پنجره‌ها 8/8% واریانس و مهم‌ترین پنجره روی کروموزوم 10، حدود 08/2% واریانس را کنترل می‌نمودند. درخصوص مقدار چربی و پروتئین شیر، به‌ترتیب تعداد 9 منطقه روی 7 کروموزوم، حدود 6/15% و 9 پنجره روی 8 کروموزوم، حدود 6/10% از  واریانس را توصیف می‌کردند. نتایج نشان داد که 4 ناحیه ژنومی اثر پلیوتروپی دارند. یافته‌های این تحقیق می‌تواند به‌عنوان منبع اطلاعاتی مهمی در ارزیابی‌های ژنومی صفات تولید شیر و تعداد سلول‌های بدنی باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Genome-wide association study for milk production and somatic cell score traits in ‎Iranian Holstein cattle

نویسندگان [English]

  • Rostam Pahlavan 1
  • Mohammad Moradi Shahre Babak 2
  • Ardeshir Nejati Javaremi 2
  • Rostam Abdollahi Arpanahi 3

1 Ph.D. Candidate in Animal Breeding and Genetics , Department of Animal Science, College of Agriculture and ‎Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Researcher, Department of Animal Science, University of Florida, USA

چکیده [English]

The main objective of dairy farmers is to maximize their profit. Increased incidence of mastitis in farms is one of the health problems, causing in serious economic losses as a consequence of treatment costs and reduction of production and longevity. The objective of this study was to evaluate the genetic architecture and associated genomic regions with milk production and somatic cell score (SCS) as an indirect measure of mastitis and the quality of raw milk. Thus, an SNP data set from 1938 Holstein bulls were used in a single-step genome-wide association study. The proportion of additive genetic variance (agv) for each of 1.5-Mb genomic window (adjacent SNPs) was used to identify informative genomic regions, accounting for more than 1% of the agv. A total of 11 significant windows over 9 bovine autosomes were found for the SCS. A peak on BTA14 explained the largest proportion of variance (3.85%). These regions together, explained 20% of agv and harbored 94 candidate genes. For milk yield, we identified 6 informative windows across 6 chromosomes, and a peak on BTA10 explained 2.08% of agv. These regions, explained 8.8% of the agv and sheltered 89 candidate genes. For the fat yield, 9 significant windows were identified on 7 chromosomes and explained 15.6% of agv, and 9 windows contained 87 candidate genes on 8 bovine autosomes were associated with milk protein yield (10.6% of agv). Four genomic regions had a pleiotropic effect. These findings can be an important source of information in genomic evaluation of dairy cattle.

کلیدواژه‌ها [English]

  • Candidate gene
  • genomic windows
  • Holstein
  • somatic cell score‎
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