بررسی تأثیر باکتری تسوکامورلا اینکوننسیس بر عیار سرمی طیور گوشتی متعاقب واکسیناسیون علیه ‏بیماری بورس عفونی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه تبریز، تبریز، ایران

2 دانش‌آموخته دکتری تخصصی طیور، کلینیک طیور، یزد، استان یزد، ایران

چکیده

مطالعه حاضر به منظور بررسی اثر باکتری غیرفعال‌شده تسوکامورلا اینکوننسیس (Tsukamurella inchonensis) بر پاسخ ایمنی حاصل از واکسیناسیون علیه بیماری بورس عفونی (Infectious bursal Disease) در طیور گوشتی انجام گرفته است. تعداد 180 قطعه جوجه گوشتی یک روزه نژاد راس به پنج گروه 36تایی تقسیم شده و از سن یک‌روزگی داخل قفس پرورش داده شدند. گروه‌های تیمار با افزودن 106 باکتری غیرفعال‌شده به جیره فراهم شدند که در گروه اول این تعداد باکتری از روز اول تا سیزده به‌صورت 24 ساعته، در دسترس طیور بودند. در گروه دوم، باکتری در روزهای 1 تا 5، 8، 9، 12 و 13 در طی روز به جیره اضافه می‌شد. گروه 3، 106 باکتری کشته­شده را به‌صورت زیرجلدی در روزهای 1، 6 و 12 دریافت کرد. گروه‌های 4 و 5 در طی دوره آزمایش باکتری دریافت نکردند. در ضمن، برای گروه پنج واکسیناسیون صورت نپذیرفت. در روزهای 1، 14، 28 و 42 از 12 پرنده در هر گروه خون‌گیری تصادفی صورت گرفت. سنجش عیار آنتی‌بادی ضدبیماری بورس عفونی (گامبورو) با استفاده از کیت های تجاری و به روش الیزای ساندویچ دوگانه صورت پذیرفت. در گروه‌های تیمار، با هر روش تجویز تسوکامورلا اینکوننسیس، عیار آنتی‌بادی واکسن گامبورو افزایش یافته بود که این افزایش در گروه دو معنی‌دار بود (05/0P˂). به نظر می‌رسد تسوکامورلا اینکوننسیس به‌ویژه در صورت مصرف متناوب، منجر به افزایش اثر واکسن گامبورو شده و در نتیجه پاسخ ضد ویروسی را تقویت می‌کند.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of inactivated Tsukamurella inchonensis on post vaccination serum titer ‎against infectious bursal disease in broiler chicks

نویسندگان [English]

  • Katayoon Nofouzi 1
  • Monireh Khordadmehr 1
  • Vahid Reza Ranjbar 2
1 Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, ‎Tabriz, Iran
2 Ph.D. Graduate, Clinic of Poultry Disease, Yazd Province, Yazd, Iran
چکیده [English]

The present study was designed to investigate the effect of inactivated Tsukamurella inchonensis (T. inchonensis) on the immune response against infectious bursal disease (IBD) vaccination in broiler chicks. Ross broiler chicks in the cage (n= 180; one day old) were randomly assigned to five groups (36 birds in each group). Experimental diets were prepared by adding 106 cells per bird of inactivated T .inchonensis into the commercial basal diet. For group the treatment was continuously dosed during 24 h from day 1 to day 13; and for group 2 during 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 106 bacteria as subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 weren't received T. inchonensis during the experiment period. Blood was collected on days 1, 14, 28, and 42 from the wing vein of 12 birds, randomly per treatment. Serum IBD antibody titer were studied by ELISA, which measured by double-antibody sandwich ELISA using commercial kits. T. inchonensis treatments, irrespective of the routes of delivery, increased the antibody titers to IBD vaccines, especially when broiler chickens treated with pulse dosed in the feed (p < 0.05). T. inchonensis augmented the effects of IBD vaccination in strengthening subsequent anti-viral responses.

کلیدواژه‌ها [English]

  • Actinomycetes
  • Immune responses
  • route of administration

مراجع

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علوم دامی ایران
دوره 51، شماره 3
آذر 1399
صفحه 193-199

فایل ها

سابقه مقاله

  • تاریخ دریافت: 02 تیر 1399
  • تاریخ بازنگری: 07 مرداد 1399
  • تاریخ پذیرش: 19 مرداد 1399

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