تغییرات غلظت استروئیدهای جنسی و بیان ژن گیرنده‌های آنها در دوره بلوغ خروس‌های بومی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، ایران

2 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، ایران

3 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، ایران

چکیده

به‌منظور بررسی تغییرات غلظت استروئیدهای جنسی و بیان ژن گیرنده‌های آنها در بیضه­های خروس­های بومی فارس، 24 قطعه خروس 5، 6، 7 و 8 ماهه به‌طور تصادفی انتخاب شدند. در هر نوبت نمونه­برداری شش خروس کشتار و نمونه‌های خون برای اندازه­گیری غلظت هورمون­های تستوسترون، استرادیول و پروژسترون و نمونه‌های بافت بیضه برای اندازه‌گیری میزان بیان ژن گیرنده‌های این هورمون­های جنسی جمع­آوری شدند. نتایج نشان داد که غلظت تستوسترون و پروژسترون خون  خروس­ها در سن 7 و 8 ماهگی به‌طور معنی‌داری بیشتر از سن 5 و 6 ماهگی بود. بیشترین غلظت استرادیول خون در 5 ماهگی و کمترین آن در 7 و 8 ماهگی مشاهده شد. میزان بیان ژن گیرنده‌های تستوسترون و استرادیول در بیضه خروس­های 6، 7 و 8 ماهه نسبت به خروس­های 5 ماهه افزایش یافت ولی میزان بیان ژن گیرنده پروژسترونی در خروس­های 6 ماهه نسبت به 5 ماهه کاهش و در خروس­های 7 و 8 ماهه نسبت به خروس­های 5 ماهه تغییر نکرد. نتایج به‌طور کلی نشان داد که غلظت هورمون تستوسترون و بیان ژن گیرنده آن در دوره بلوغ‌جنسی خروس­های بومی فارس افزایش و علی‌رغم کاهش غلظت هورمون استرادیول بیان ژن گیرنده آن در این دوره افزایش یافت.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation the changes of sexual steroids concentrations and their receptors gene ‎expression during the sexual maturity of native roosters

نویسندگان [English]

  • Sina Looharii Yeilaghi 1
  • Mehrdad Meamar 2
  • Mostafa Mohaghegh Dolatabadi 3
  • Reza Naghiha najaf Abadi 2
1 M.Sc. Student, Department of Animal Science, Yasouj ‎University, Yasouj, Iran
2 Assistant Professor, Department of Animal Science, Yasouj University, Yasouj, Iran‎
3 Associate Professor, Department of Animal Science, Yasouj University, Yasouj, Iran‎
چکیده [English]

In order to investigate the changes of sexual steroids concentrations and expression of their receptors’ gene in the testes of Fars native roosters, 24 roosters in the age of 5, 6, 7 and 8 months were selected randomly. In each date of sampling, 6 roosters were slaughtered and blood samples for measuring the concentration of testosterone, estradiol and progesterone, and testis samples for evaluating the level of receptors gene expression of these sexual hormones, were collected. The results indicated that testosterone and progesterone concentrations in the blood of 7 and 8-month roosters were significantly higher compared to 5 and 6-month roosters. The maximum concentration of estradiol in blood was observed in 5 –month roosters and the minimum concentration was observed in 7 and 8-month roosters. The level of testosterone and estradiol receptors gene expression in the testes of 6, 7 and 8-month roosters were increased significantly compared to 5-month roosters, but the level of progesterone receptor gene expression decreased in 6-month roosters compared to 5-month ones. Progesterone receptor gene expression in 7 and 8-month roosters was not changed significantly compared to 5-month roosters. In conclusion the results indicated that both of the testosterone concentration in blood and the expression of its receptor gene in testis, were increased during the sexual maturity period of Fars native roosters but the estradiol concentration decreased while its receptor gene expression increased in the mentioned period.

کلیدواژه‌ها [English]

  • Estradiol
  • gene expression
  • roosters
  • testosterone
  • testosterone and estradiol receptors‎

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علوم دامی ایران
دوره 51، شماره 1
اردیبهشت 1399
صفحه 37-44

فایل ها

سابقه مقاله

  • تاریخ دریافت: 10 مهر 1398
  • تاریخ بازنگری: 03 فروردین 1399
  • تاریخ پذیرش: 06 اردیبهشت 1399

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