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آثار جاذب‌های مختلف بر عملکرد و فراسنجه‌های کبدی جوجه‌های گوشتی تغذیه‌شده با جیره‌های ‏آلوده به آفلاتوکسین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران، ایران

چکیده

این آزمایش جهت بررسی اثر آلومینوسیلیکات، اسید هیومیک، دیواره سلولی مخمر، پودر گیاهی و یک توکسین بایندر تجاری برای کاهش آثار آفلاتوکسین B1 در جیره جوجه‌های گوشتی انجام شد. آزمایش با استفاده از 320 قطعه جوجه­­گوشتی در قالب طرح کاملاً تصادفی با 8 تیمار، 4 تکرار و 10 قطعه پرنده در هر تکرار از سن 7 تا 28 روزگی انجام شد. تیمار­های آزمایشی شامل: 1) شاهد منفی (بدون آفلاتوکسین)، 2) شاهد مثبت (حاوی 3/0 میلی­گرم آفلاتوکسینB1 در کیلوگرم جیره)، 3) شاهد مثبت + آلومینوسیلیکات، 4) شاهد مثبت+ آلومینوسیلیکات+ اسید هیومیک، 5) شاهد مثبت+ آلومینوسیلیکات+ دیواره سلولی مخمر، 6) شاهد مثبت+ آلومینوسیلیکات+ اسید هیومیک+ دیواره سلولی مخمر، 7) شاهد مثبت+ توکسین بایندر تجاری مگنوتوکس و 8) شاهد مثبت+ پودر گیاهی بود. استفاده از جیره حاوی آفلاتوکسین سبب کاهش عملکرد رشد جوجه‌ها، افزایش وزن کبد، قلب و پانکراس و کاهش غلظت آلبومین، پروتئین کل و گلوکز سرم شد (05/0>P). افزودن آلومینوسیلیکات به‌عنوان جاذب به تنهایی در کاهش آثار منفی آفلاتوکسین مؤثر بود. افزودن دیواره سلولی مخمرسبب تقویت اثرات آلومینوسیلیکات شد، اما افزودن اسید هیومیک زیاد مؤثر نبود. در مجموع، به نظر می­ رسد ترکیب آلومینوسیلیکات و دیواره سلولی مخمر بیشترین جذب آفلاتوکسین B1 را دارا می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of different adsorbents on the performance and liver parameters of broilers ‎fed diets contaminated with aflatoxin ‎

نویسندگان [English]

  • Moloud Parsafar 1
  • Maziar Mohiti Asli 2
  • Mohsen Farzaneh 3
1 M.Sc. Student, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht ‎‎41635-1314, Iran‎
2 Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht 41635-1314, Iran‎
3 Assistant Professor, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran 19835-389, Iran‎
چکیده [English]

The experiment was carried out to investigate the effect of aluminum silicate (AS), humic acid (HA), Saccharomyces cerevisiae cell wall (SC), herbal powder (HP) and a commercial toxin binder to alleviate the effects of aflatoxin B1 (AFB1) in broiler diet.  In this experiment we used 320 day old chicks in a completely randomized design with 8 treatments and 4 replications, and 10 birds in each replicate, from 7 to 28 days of age. Experimental treatments were: 1) negative control (NC; without AFB1), 2) positive control (PC; contaminated by 0.3 mg AFB1 / kg diet), 3) PC + AS, 4) PC + AS + HA, 5) PC + AS + SC, 6) PC + AS + HA + SC, 7) PC + Magnotox as a commercial binder and 8) PC + HP. Feeding AFB1 contaminated diet reduced broiler performance, increased relative weights of liver, heart, pancreas and reduced serum albumin, total protein and glucose concentrations (P <0.05). Inclusion of AS in PC diet individually improved the negative effects of AFB1. However, supplementation of SC boosted AS effects, HA supplementation was rarely effective. Finally, it can be concluded that the combination of AS + SC has the highest adsorbing ability of AFB1.

کلیدواژه‌ها [English]

  • Aflatoxin B1
  • Aluminum silicate
  • Broilers
  • Humic Acid
  • ‎‏ ‏Yeast cell wall‎
  1. Alam, M. J., Howlider, M. A. R., Pramanik, M. A. H. & Haque, M. A. (2003). Effect of exogenous enzyme in diet on broiler performance. International Journal of Poultry Science, 2, 168-173.
  2. AOAC International. (2005). Association of Official Analysis Chemists, (18th ed.). AOAC, Arlington, VA.
  3. Aravind, K. L., Patil, V. S., Devegowda, G., Umakantha, B. & Ganpule, S. P. (2003). Efficacy of esterified glucomannan to counteract mycotoxicosis in naturally contaminated feed on performance and serum biochemical and hematological parameters in broilers. Poultry Science, 82, 571-576.
  4. Bailey, R. H., Kubena, L. F., Harvey, R. B., Buckley, S. A. & Rottinghaus, G. E. (1998). Efficacy of various inorganic sorbents to reduce the toxicity of aflatoxin and T-2 toxin in broiler. Poultry Science, 77, 1623-1630.
  5. Daghir, N.J. (1995). Mycotoxins in Poultry Feeds, Poultry Production in Hot Climates. CAB International,pp, 157-184.
  6. Devegowda, G. & Raju, M.V. (1998). Mycotoxins: Novel solutions for their counteraction. Feedstuffs, 370, 12-16.
  7. Dos Anjos, F., Ledoux, D. R., Rottinghaus, G. E. & Chimonyo, M. (2016). Efficacy of Mozambican bentonite and diatomaceous earth in reducing the toxic effects of aflatoxins in chicks. World Mycotoxin Journal, 9, 63-72.
  8. Eraslan, G., Eşsiz, D., Akdoğan, M., Şahindokuyucu, F. & Altintaş, L. (2005). The effects of aflatoxin and sodium bentonite combined and alone on some blood electrolyte levels in broiler chickens. Turkish Journal of Veterinary and Animal Sciences, 29, 601-605.
  9. Gorran, A., Farzaneh, M., Shivazad, M., Rezaeian, M. & Ghassempour, A. (2013). Aflatoxin B1-reduction of Aspergillus flavus by three medicinal plants (Lamiaceae). Food Control, 31, 218-223. (in Farsi)
  10. Hoerr, F. J. (2003). Mycotoxicoses. Diseases of Poultry. (11th ed.). Y. M. Saif, H. J., Barnes, C. W., Beard, J. R. & Glisson, A. M. Fadly, L. R. & Osweiler, G. (2005). Pages 1103–1132. Veterinary Diagnostic Laboratory Iowa State University, Ames, Iowa.
  11. Islam, K. M. S., Schuhmacher, A. & Gropp, J. M. (2005). Humic acid substances in animal agriculture. Pakistan Journal of Nutrition, 4, 126-134.
  12. McGavin, M.O., Cartton, W.W. & Zachary, J.F. (2001). Thomsons Special Veterinary Pathology. (3rd ed.), MoASy, St. Louis, USA. Pp. 110.
  13. Neeff, D.V., Ledoux, D.R., Rottinghaus, G.E., Bermudez, A.J., Dakovic, A., Murarolli, R.A. & Oliveira, C.A.F. (2013). In vitro and in vivo efficacy of a hydrated sodium calcium aluminosilicate to bind and reduce aflatoxin residues in tissues of broiler chicks fed aflatoxin B1. Poultry Science, 92, 131-137.
  14. Oliveira, C. A. F., Kobashigawa, E., Reis, T. A., Mestieri, L., Albuquerque, R. & Correa, B. (2000). Aflatoxin B1 residues in eggs of laying hens fed a diet containing different levels of the mycotoxin. Food Additive Contamination, 17, 459-462.
  15. Parizadian-Kavan, B., Shams-Shargh, M., Hassani, S. & Mostafalo, U. (2015). Study of the effect of physical size of clinoptilolite on liver histology, carcass traits and blood enzymes activity of broilers fed rations contaminated with aflatoxin. Veterinary Journal (Pajouhesh-va-Sazandegi) 28, 35-44. (in Farsi)
  16. Pizzolitto, R. P., Armando, M. R., Salvano, M. A., Dalcero, A. M. & Rosa, C. A. (2013). Evaluation of Saccharomyces cerevisiae as an antiaflatoxicogenic agent in broiler feedstuffs. Poultry Science, 92, 1655-1663.
  17. Raju, M. V. & Devegowda, G. (2000). Influence of esterified glucomannan on performance and organ morphology, serum biochemistry and hematology in broiler exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T2 toxin). British Poultry Science, 41, 640-650.
  18. Ramos, A. J. & Hernandez, E. (1996). In vitro aflatoxin adsorption by means of montmorillonite silicate. A study of adsorption isotherms. Animal Feed Science and Technology, 62, 263-269.
  19. Razzaghi-Abyaneh, M., Shams-Ghahfarokhi, M., Yoshinari, T., Rezaee, M, B., Nagasawa, H. & Sakuda, S. (2008). Inhibitory effects of Satureja hortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus. International Journal of Food Microbiology, 123, 228-233. (in Farsi)
  20. Safamehr, A., Allameh, A., Shivazad, M., Moradi, M. & Mirhadi, A. (2005). Study of liver pathology lesions and performance of broiler chickens fed with contaminated corn by aflatoxin and ammoniac. Iranian Journal of Agricultural Science, 36, 1295-1303. (in Farsi)
  21. Santin, E., Paulillo, A.C., Maiorka, A., Nakaghi, L.S.O., Macari, M., Silva, A.V.F. & Alessi, A.C. (2003). Evaluation of the efficacy of Saccharomyces cerevisiae cell wall to ameliorate the toxic effects of aflatoxin in broilers. International Journal of Poultry Science, 2(5), 341-344.
  22. SAS Institute. (2003). SAS User’s Guide. Release Version 9/1. SAS Institute Inc., Cary, NC.
  23. Shannon, T.A., Ledoux, D.R., Rottinghaus, G.E., Shaw, D.P., Dakovic, A. & Markovi, M. (2016). The efficacy of raw and concentrated bentonite clay in reducing the toxic effects of aflatoxin in broiler chicks. Poultry Science, 96, 1651-1658.
  24. Stanley, V.G., Ojo, R., Woldesenbet, S. & Hutchinson, D. H. (1993). The use of Sacchoromyces cerevisiae to suppress the effects of aflatoxicosis in broiler chicks. Poultry Science, 72, 1867-1872.
  25. USDA. Giblets and Food Safety. (2008). Food Safety and Inspection Service. United States Department of Agriculture: Philadelphia, PA, USA, pp: 1-2.
  26. van Rensburg, C. J., Van Rensburg, C. E. J., Van Ryssen, J. B. J., Casey, N. H. & Rottinghaus, G. E. (2006). In vitro and in vivo assessment of humic acid as an aflatoxin binder in broiler chickens. Poultry Science, 85, 1576-1583.
  27. Yarru, L.P., Settivari, R.S., Antoniou, E., Ledoux, D.R. & Rottinghaus, G.E. (2009). Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male chicks. Poultry Science, 88, 360-371.
  28. Yoruk, M.A., Gul, M., Hayirli, A. & Macit, M. (2004). The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poultry Science, 83, 84-88.