تأثیر کورکومین بر فراسنجه‌های کیفی و باروری اسپرم خروس‌های مادرگوشتی پس از یخ‌گشایی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ‏ایران

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 استادیار بخش تحقیق، پرورش‎ ‎و تولید حیوانات آزمایشگاهی، مؤسسه تحقیقات واکسن و سرم‌‌سازی رازی، سازمان تحقیقات، ‏آموزش و ترویج کشاورزی، کرج، ایران

5 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

هدف این پژوهش، مطالعه اثر تغذیه کورکومین بر فراسنجه‌های کیفی اسپرم و باروری خروس­های مادرگوشتی پس از یخ‌گشایی‌ بود. تعداد 20 قطعه خروس مادرگوشتی سویه راس 308 در سن 49 هفتگی به­طور تصادفی به چهار گروه آزمایشی (5n =) تقسیم شدند. سطوح مختلف کورکومین شامل: صفر (T1)، 10 (T2)، 20 (T3) و 30 (T4) میلی‌گرم کورکومین به­ازای هر پرنده در هر روز به جیره پایه اضافه و از 49-61 هفتگی به پرنده­ها تغذیه شد. پس از گذشت یک دوره پنج هفته­ای از تغذیه کورکومین (49-53 هفتگی)، فراسنجه­های کیفی اسپرم طی شش هفته (54 تا 59 هفتگی) پس از یخ­گشایی ارزیابی شد. نمونه‌های منی هفته­های 60 و 61 پس از یخ‌گشایی برای ارزیابی باروری، به 68 مرغ مادر گوشتی (17n=) تلقیح شد. نتایج نشان داد جنبایی کل در تیمارهای T3 و T4 و جنبایی پیش‌رونده در تیمارهای T2، T3 و T4 نسبت به T1 افزایش یافت (05/0>P). تغذیه کورکومین زنده‌مانی اسپرم را پس از یخ­گشایی در تیمارهای T3 و T4 نسبت به T1 افزایش داد (05/0>P). عملکرد غشای پلاسمایی (HOS) در تمامی‌گروه‏‌‏های تیماری دارای کورکومین نسبت به تیمار­ T1 افزایش یافت (05/0>P)، به‌طوری که بیشترین عملکرد مربوط به تیمارهای T3 و T4 بود. نرخ باروری اسپرم در تیمارهای T3 و T4 نسبت به T1 افزایش یافت (05/0>P). در کل، نتایج این پژوهش نشان از تأثیرات مثبت تغذیه کورکومین بر کیفیت اسپرم و باروری خروس­های مادرگوشتی پس از یخ‌گشایی داشت و بهترین عملکرد زمانی حاصل شد که روزانه 20 یا 30 میلی­گرم کورکومین به‌ازای هر پرنده به‌مدت 13 هفته تغذیه شد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of curcumin on frozen-thawed sperm quality and fertility of broiler ‎breeder roosters

نویسندگان [English]

  • Faezeh Jalili 1
  • Ahmad Zareh-Shahneh 2
  • Saeed Zeinoaldini 3
  • Ali Reza Yousefi 4
  • Amin Kazemizadeh 5
1 M.Sc. Student, Department of Animal Science, College of Agriculture & Natural Resources, ‎University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associated Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Assistant Professor, Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research ‎Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5 M.Sc. Student, Department of Animal Science, College of Agriculture & Natural Resources, ‎University of Tehran, Karaj, Iran
چکیده [English]

The aim of this study was to investigate the effect of curcumin on post-thawed sperm quality parameters and fertility of male broiler breeders. Twenty 49-week-old Ross 308 broiler roosters were randomly divided into four experimental groups (n=5). Different levels of curcumin including: 0 (T1), 10 (T2), 20 (T3) and 30 (T4) mg of curcumin/bird/day were supplemented to basal diet and fed to the birds from 49-61 weeks of age. After a 5-week of feeding curcumin (49-53 weeks), sperm quality was assessed for 6 weeks (54 to 59 weeks of age) following cryopreservation of semen samples. In order to evaluate fertility rate, the semen samples from weeks 60 and 61 were thawed and artificially inseminated into 68 broiler hens (n=17). Curcumin supplementation increased total motility in the T3 and T4 groups, and progressive motility in the T2, T3, and T4 groups compared to the T1 group (P<0.05). Feeding curcumin increased the viability of sperm in T3 and T4 groups compared to the T1 group (P<0.05). Plasma membrane functionality (HOS) was increased in all curcumin-treated birds compared to the control group (P<0.05); however, the highest performance was observed in T3 and T4 groups. Curcumin supplementation increased fertility rate in T3 and T4 groups compared to the T1 group. In general, the results of this study indicated positive effects of curcumin on sperm quality and fertility of broiler breeder roosters after thawing, and the best results were obtained when 20 or 30 mg of curcumin were fed daily for 13 weeks.

کلیدواژه‌ها [English]

  • Antioxidant
  • cryopreservation sperm
  • ‎‏ ‏rooster
  • turmeric

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علوم دامی ایران
دوره 50، شماره 4
اسفند 1398
صفحه 295-306

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  • تاریخ دریافت: 07 اسفند 1396
  • تاریخ بازنگری: 09 اردیبهشت 1397
  • تاریخ پذیرش: 11 اردیبهشت 1397

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