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شناسایی ژن‌های مؤثر بر میزان چربی محوطۀ بطنی جوجه‏‌‏های گوشتی با استفاده از داده‌های ‏ریزآرایه و توالی‌یابی ‏RNA

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، ژنتیک و اصلاح نژاد دام گروه علوم دامی، پردیس کشاورزی ‏و منابع طبیعی، دانشگاه تهران، کرج، ایران

2 دانشیار ژنتیک و اصلاح نژاد دام گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه ‏تهران، کرج، ایران

3 دانشجوی سابق دکتری ژنتیک و اصلاح نژاد دام گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ‏ایران

4 استاد ژنتیک و اصلاح نژاد دام گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

امروزه انتخاب ژنتیکی برای افزایش رشد و تولید پروتئین حیوانی با کیفیت بالا در جوجه‏‌‏های گوشتی (Gallus gallus domesticus)، معمولاً منجر به افزایش وزن بیش از حد خواهد شد که نتیجۀ آن تأثیر منفی بر راندمان مصرف خوراک و کیفیت لاشه مانند افزایش چربی محوطه بطنی می­شود. هدف این مطالعه استفاده از پروفایل ترانسکریپتوم بافت چربی دو دسته جوجه‏‌‏های گوشتی با چربی زیاد و کم در محوطۀ بطنی، به‏‌‏منظور شناسایی ژن­های مؤثر در متابولیسم و ذخیره چربی می­باشد. در تجزیه داده­های ریزآرایه و RNA-seq برای بیان تفاوت ژنی به­ترتیب 2914 و 1867 ژن استخراج شد که در مجموع با مقایسه ژن­های شناسایی­شده که تفاوت بیانی معنی­داری داشتند (000001/0P<)، 1835 ژن شناسایی شد. سپس با مقایسه تعداد ژن مربوطه در میان پروفایل­های ترانسکریپتوم، ژن­های مشترک مرتبط شامل THBS1، COLEC12، ANXA7، RGS19، TMEM258 و HTR7L بودند که در فرآیند اصلی مسیرهای کنترل سنتز، متابولیسم و ذخیره چربی و مسیر سیگنالینگ غدد درون­ریز فعال شده توسط آدیپوکین­ها دارای نقش می­باشند. تجزیه و تحلیل ترانسکریپتوم مرتبط با ذخیره چربی محوطه بطنی می­تواند بیان­کننده نقش آن به‏‌‏عنوان یک اندام متابولیک و درون­ریز در جوجه‏‌‏های گوشتی باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of genes affecting the amount of abdominal fat in broiler chickens ‎using microarray and RNA sequencing data

نویسندگان [English]

  • Farzad Ghafouri 1
  • Mostafa Sadeghi 2
  • Abolfazl Bahrami 3
  • Seyed Reza Miraei Ashtiani 4
1 M.Sc. Student, of Animal Breeding and Genetics, Department of Animal ‎Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor of Animal Breeding and Genetics, Department of Animal Science, ‎College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Former Ph.D. Student of Animal Breeding and Genetics, Department of Animal Science, College of Agriculture and ‎Natural Resources, University of Tehran, Karaj, Iran
4 Professor of Animal Breeding and Genetics, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, ‎Karaj, Iran
چکیده [English]

Abdominal fat deposition and several other unique features in the metabolism of birds such as interaction between genetic and endocrine factors, fasting hyperglycemia and insulin resistance are signs of obesity and metabolic disorders in poultry, similar to humans. The main purpose of this study was to use transcript profile of fat tissue in two groups of broiler chickens with high and low abdominal fat deposition, to identify the genes involved in storage and metabolism of fat, as well as the signaling pathways associated with the endocrine glands. Based on the analysis of microarray and RNA-seq data, 2914 and 1867 genes were detected as differentially expressed genes, respectively. In total, 1835 genes were identified by comparing the genes with a significant difference in expression (P<0.000001). Then, by comparing the number of relevant genes among the transcript profiles, the most important related genes were THBS1, COLEC12, ANXA7, RGS19, TMEM258 and HTR7L, which in the main process of pathways controlling synthesis, fat metabolism and storage and the endocrine signaling pathways activated by adipokines, are involved. The analysis of the relevant tissue may indicate the role of ventricular fat as a metabolic and endocrine organ in broiler chickens.

کلیدواژه‌ها [English]

  • Data Mining
  • Fat Metabolism
  • signaling pathway
  • transcriptomic profile
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