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مقایسه میزان هوموزیگوسیتی در جایگاه‌های SNP و ROH در گاوهای شیری پر ‌تولید و کم‌ تولید هلشتاین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری ژنتیک و اصلاح نژاد دام، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع‌طبیعی دانشگاه تهران، کرج

3 استاد، گروه علوم‌دامی، پردیس کشاورزی و منابع‌طبیعی دانشگاه تهران، کرج

4 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه ایالتی آیووا، ایالات متحده امریکا

چکیده

افزایش هوموزیگوسیتی حاصل از آمیزش حیوانات خویشاوند، به­عنوان یکی از چالش­های فرا روی صنعت پرورش گاو شیری می­باشد که توجه زیادی را به خود جلب کرده است. پژوهش حاضر به ­منظور بررسی هوموزیگوسیتی بر مبنای جایگاه­های SNP و ROH در دام­های پر ­تولید و کم ­تولید هلشتاین انجام شده است. در این مطالعه، برای تعیین حیوانات کاندیدا برای تعیین ژنوتیپ از دو رویکرد تابعیت تصادفی و شاخص شجره­ای استفاده شد. برای این منظور، نمونه­گیری از 150 رأس گاو شیری (75 دام با ارزش اصلاحی بالا و 75 دام با ارزش اصلاحی پایین برای صفت تولید شیر) انجام شد. یک روش بهینه به­ منظور انتخاب حیوانات کاندیدا برای تعیین ژنوتیپ، از طریق تلفیق برآوردهای حاصل از تابعیت تصادفی و شاخص شجره­ای ارائه گردید. ارزش­های اصلاحی ژنومی برای دو گروه نشان داد که تاکید بر افزایش سطح تولید در دام­های پر تولید، اثر منفی بر روی صفات مرتبط با باروری (55/0- = DPR) و طول­عمر تولیدی (1/0=PL) داشته است. همچنین، میزان هوموزیگوسیتی بر مبنای ROH دامنه­ای از تغییرات را برای کروموزوم­های مختلف در دام­های با سطح تولید بالا و پایین نشان داد که ممکن است با تفاوت در سطح تولید این دو گروه و پراکنش غیر یکنواخت ژن­های مؤثر بر میزان تولید بر روی کروموزوم­های مختلف در ارتباط باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of homozygosity in SNP and ROH sites in Holstein dairy cows with high ‎and low production levels

نویسندگان [English]

  • Hojatollah Mousapour 1
  • Ardeshir Nejati-Javaremi 2
  • Mohammad Moradi-ShahreBabak 3
  • Mahdi Saatchi 4
1 Ph.D. Candidate in Animal Breeding and Genetics, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Assistant Professor in Animal Breeding and Genetics, Department of Animal Science, Iowa State University, USA
چکیده [English]

Increased homozygosity resulted from mating of relatives is considered as one of the challenges faced in dairy farming industry which has attracted the attentions. This research has been conducted to measure homozygosity based on SNP and ROH in high- and low-producing Holstein cows. In current research, both random regression and pedigree index approaches were used to obtain candidate animals for genotyping process. The samples were obtained from 150 Holstein dairy cows (75 by high- and 75 by low-EBV for milk production). We proposed a suitable method, by integrating breeding value estimation calculated by random regression and pedigree index, to select the candidate animals for genotyping. The results showed that putting too much emphasis on production traits in high-producing dairy cows had negative impact on the traits associated with fertility (DPR = -0.55) and productive life (PL = 0.1). The calculated homozygosity based on ROH showed different amount of variation in different chromosomes in the cows with high and low production which may be related to uneven distribution of genes influencing production traits in different chromosomes.

کلیدواژه‌ها [English]

  • Dairy cattle
  • fertility
  • Genomic information
  • inbreeding
  • Homozygosity
  • Productive life
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