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بهبود فراسنجه‌های بافت‌شناسی و بیان نسبی ژن StAR در بیضه خروس‌های تغذیه‌شده با کریسین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه کشاورزی و منابع طبیعی ساری، ساری

چکیده

در این پژوهش اثر کریسین خوراکی بر بافت­شناسی و بیان نسبی StAR در بیضه خروس­های راس 308 تغذیه­شده با کریسین بررسی شد. بیست خروس (40 هفته) به صورت تصادفی به چهار گروه مساوی تقسیم شدند و جیره پایه به اضافه سطوح متفاوتی از کریسین شامل صفر (Ch0)، 25 (Ch25)، 50 (Ch50) و 75 (Ch75) میلی­گرم در روز به‌مدت 12 هفته متوالی دریافت کردند. در پایان آزمایش خروس­ها کشتار و بیضه ها به دقت خارج شدند. دو نمونه از یک بیضه برداشته شد، نمونه اولی برای مطالعه بافت­شناسی به درون محلول بوئن و نمونه دوم برای ارزیابی بیان نسبی ژن به درون ازت مایع منتقل شد. نتایج نشان داد که قطر و ضخامت اپیتلیوم لوله اسپرم­ساز (05/0p<) و تعداد اسپرماتوگونی­ها (01/0p<) در دو گروه Ch50 و Ch75 نسبت به شاهد به‌طور معنی­داری افزایش یافت، اما تعداد سلول­های لایدیگ و رگ‌های خونی بین گروه­های آزمایشی تفاوت معنی­داری نداشت. بیان نسبی ژن StAR در پرندگانی که 75 میلی­گرم کریسین در روز دریافت کرده بودند، نسبت به سایر گروه­های تیماری به‌طور معنی­داری افزایش یافت (05/0p<). به‌طور خلاصه، در این پژوهش کریسین خوراکی توانست فراسنجه­های بافت شناسی و مسیر استروئیدسازی را در بیضه خروس­ها بهبود دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of testis histological parameters and relative expression of StAR gene in Chrysin-fed roosters

نویسندگان [English]

  • Ali Sami Amin Altawash 1
  • Ahmad Zareh Shahneh 2
  • Hossein Moravej 2
  • Mahdi Ansari 1
  • Hamid Deldar 3
1 Former Ph.D. Student, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Science Natural Resources University, Sari, Iran
چکیده [English]

Chrysin was orally administered to evaluate testicular histology and relative StAR expression of Ross 308 roosters in the current study. Twenty roosters were randomly divided into four groups and were subjected to the basal diet supplemented with different levels of Chrysin including 0 (Ch0), 25 (Ch25), 50 (Ch50) or 75 (Ch75) mg/bird/day for 12 successive weeks. At the end of trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen with liquid nitrogen to assess relative gene expression. According to the results, seminiferous tubule and epithelium diameters (p<0.05) and spermatogonial numbers (p<0.01) of both CH75 and Ch50 groups were significantly improved compared to control group. However, Leydig cell numbers and blood vessels were not significantly affected. Birds fed 75 mg of Chrysin per day had significantly higher StARtranscript level compared to other groups (p<0.05). In conclusion, oral administration of Chrysin to roosters could improve testicular histology and steroidogenic parameters in the current study.

کلیدواژه‌ها [English]

  • Chrysin
  • Gene
  • Histology
  • Rooster
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