نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی سابق دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
3 استادیار، گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه کشاورزی و منابع طبیعی ساری، ساری
چکیده
در این پژوهش اثر کریسین خوراکی بر بافتشناسی و بیان نسبی StAR در بیضه خروسهای راس 308 تغذیهشده با کریسین بررسی شد. بیست خروس (40 هفته) به صورت تصادفی به چهار گروه مساوی تقسیم شدند و جیره پایه به اضافه سطوح متفاوتی از کریسین شامل صفر (Ch0)، 25 (Ch25)، 50 (Ch50) و 75 (Ch75) میلیگرم در روز به‌مدت 12 هفته متوالی دریافت کردند. در پایان آزمایش خروسها کشتار و بیضه ها به دقت خارج شدند. دو نمونه از یک بیضه برداشته شد، نمونه اولی برای مطالعه بافتشناسی به درون محلول بوئن و نمونه دوم برای ارزیابی بیان نسبی ژن به درون ازت مایع منتقل شد. نتایج نشان داد که قطر و ضخامت اپیتلیوم لوله اسپرمساز (05/0p<) و تعداد اسپرماتوگونیها (01/0p<) در دو گروه Ch50 و Ch75 نسبت به شاهد به‌طور معنیداری افزایش یافت، اما تعداد سلولهای لایدیگ و رگ‌های خونی بین گروههای آزمایشی تفاوت معنیداری نداشت. بیان نسبی ژن StAR در پرندگانی که 75 میلیگرم کریسین در روز دریافت کرده بودند، نسبت به سایر گروههای تیماری به‌طور معنیداری افزایش یافت (05/0p<). به‌طور خلاصه، در این پژوهش کریسین خوراکی توانست فراسنجههای بافت شناسی و مسیر استروئیدسازی را در بیضه خروسها بهبود دهد.
کلیدواژه‌ها
عنوان مقاله [English]
Improvement of testis histological parameters and relative expression of StAR gene in Chrysin-fed roosters
نویسندگان [English]
- Ali Sami Amin Altawash 1
- Ahmad Zareh Shahneh 2
- Hossein Moravej 2
- Mahdi Ansari 1
- Hamid Deldar 3
1 Former Ph.D. Student, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Animal Science Department, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Department of Animal Science, College of Animal Science and Fisheries, Sari Agricultural Science Natural Resources University, Sari, Iran
چکیده [English]
Chrysin was orally administered to evaluate testicular histology and relative StAR expression of Ross 308 roosters in the current study. Twenty roosters were randomly divided into four groups and were subjected to the basal diet supplemented with different levels of Chrysin including 0 (Ch0), 25 (Ch25), 50 (Ch50) or 75 (Ch75) mg/bird/day for 12 successive weeks. At the end of trial, all birds were killed and two samples were collected from the same testicle one of which was processed for histology, whereas another was snap-frozen with liquid nitrogen to assess relative gene expression. According to the results, seminiferous tubule and epithelium diameters (p<0.05) and spermatogonial numbers (p<0.01) of both CH75 and Ch50 groups were significantly improved compared to control group. However, Leydig cell numbers and blood vessels were not significantly affected. Birds fed 75 mg of Chrysin per day had significantly higher StARtranscript level compared to other groups (p<0.05). In conclusion, oral administration of Chrysin to roosters could improve testicular histology and steroidogenic parameters in the current study.
کلیدواژه‌ها [English]
- Chrysin
- Gene
- Histology
- Rooster
- Aitken, R. J. & Roman, S. D. (2008). Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity, 1, 15-24.
- Ali, E. A., Zhandi, M., Towhidi, A., Zaghari, M., Ansari, M., Najafi, M. & Deldar, H. (2017). Letrozole, an aromatase inhibitor, reduces post-peak age-related regression of rooster reproductive performance. Animal Reproduction Science, 183, 110-117.
- Amin Altawash, A. S., Shahneh, A. Z., Moravej, H. & Ansari, M. (2017). Chrysin-induced sperm parameters and fatty acid profile changes improve reproductive performance of roosters. Theriogenology, 104, 72-79.
- Borghei-Rad, S. M., Zeinoaldini, S., Zhandi, M., Moravej, H. & Ansari, M. (2017). Feeding rosemary leaves powder ameliorates rooster age-related subfertility. Theriogenology, 101, 35-43.
- Campbell, D. R., & Kurzer, M. S. (1993). Flavonoid inhibition of aromatase enzyme activity in human preadipocytes. The Journal of Steroid Biochemistry and Molecular Biology, 46, 381-8.
- Ciftci, O., Ozdemir, I., Aydin, M. & Beytur, A. (2012). Beneficial effects of chrysin on the reproductive system of adult male rats. Andrologia, 44, 181-6.
- Dhawan, K., Kumar, S. & Sharma, A. (2002). Beneficial effects of chrysin and benzoflavone on virility in 2-year-old male rats. Journal of Medicinal Food,5, 43-8.
- Dimitriadis, F., Tsiampali, C., Chaliasos, N., Tsounapi, P., Takenaka, A. & Sofikitis, N. (2015). The Sertoli cell as the orchestra conductor of spermatogenesis: spermatogenic cells dance to the tune of testosterone. Hormones (Athens), 14, 479-503.
- Giudice, M. G., de Michele, F., Poels, J., Vermeulen, M. & Wyns, C. (2017). Update on fertility restoration from prepubertal spermatogonial stem cells: How far are we from clinical practice? Stem Cell Research, 21, 171-177.
- Ikeda, M., Kodama, H., Fukuda, J., Shimizu, Y., Murata, M., Kumagai, J. & Tanaka, T. (1999). Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biology of Reproduction, 61, 393-399.
- Islam, M. N., Zhu, Z. B., Aoyama, M. & Sugita, S. (2010). Histological and morphometric analyses of seasonal testicular variations in the Jungle Crow (Corvus macrorhynchos). Anatomical Science International, 85, 121-129.
- Jana, K., Yin, X., Schiffer, R. B., Chen, J. J., Pandey, A. K., Stocco, D. M., Grammas, P. & Wang, X. (2008). Chrysin, a natural flavonoid enhances steroidogenesis and steroidogenic acute regulatory protein gene expression in mouse Leydig cells. Journal of Endocrinology, 197, 315-23.
- Livak, K. J. & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative 2 -∆∆Ct Method. Methods, 25, 402-8.
- Luo, L., Chen, H., Trush, M. A., Show, M. D., Anway, M. D. & Zirkin, B. R. (2006). Aging and the brown Norway rat Leydig cell antioxidant defense system. Journal of Andrology, 27, 240-7.
- Luo, L., Chen, H. & Zirkin, B. R. (2001) Leydig cell aging: steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme. Journal of Andrology,22, 149-56.
- Mantawy, E. M., El-Bakly, W. M., Esmat, A., Badr, A. M. & El-Demerdash, E. (2014). Chrysin alleviates acute doxorubicin cardiotoxicity in rats via suppression of oxidative stress, inflammation and apoptosis. European Journal of Pharmacology, 728, 107-18.
- Midzak, A. S., Chen, H., Papadopoulos, V. & Zirkin, B. R. (2009). Leydig cell aging and the mechanisms of reduced testosterone synthesis. Molecular and Cellular Endocrinology, 299, 23-31
- Rosenstrauch, A., Weil, S., Degen, A. A. & Friedländer, M. (1998). Leydig cell functional structure and plasma androgen level during the decline in fertility in aging roosters. General and Comparative Endocrinology, 109, 251-258.
- Sanderson, J. T., Hordijk, J., Denison, M. S., Springsteel, M. F., Nantz, M. H. & van den Berg, M. (2004). Induction and inhibition of aromatase (CYP19) activity by natural and synthetic flavonoid compounds in H295R human adrenocortical carcinoma cells. Toxicological Sciences, 82, 70-79.
- Sarabia Fragoso, J., Pizarro Diaz, M., Abad Moreno, J. C., Casanovas Infesta, P., Rodriguez-Bertos, A. & Barger, K. (2013). Relationships between fertility and some parameters in male broiler breeders (body and testicular weight, histology and immunohistochemistry of testes, spermatogenesis and hormonal levels). Reproduction in Domestic Animals, 48, 345-52
- Surai, P., Noble, R., Sparks, N. & Speake, B. (2000). Effect of long-term supplementation with arachidonic or docosahexaenoic acids on sperm production in the broiler chicken. Journal of Reproduction and Fertility, 120, 257-264.
- Thurston, R. J. & Korn, N. (2000). Spermiogenesis in commercial poultry species: anatomy and control. Poultry Science, 79, 1650-68.
- Turner, T. T. & Lysiak, J. J. (2008). Oxidative stress: a common factor in testicular dysfunction. Journal of Andrology, 29, 488-98.
- Weil, S., Degen, A. A., Friedlander, M. & Rosenstrauch, A. (1999a). Low fertility in aging roosters is related to a high plasma concentration of insulin and low testicular contents of ACTH and lactate. General and Comparative Endocrinology, 115, 110-5.
- Weil, S., Rozenboim, I., Degen, A.A., Dawson, A., Friedländer, M. & Rosenstrauch, A. (1999b). Fertility decline in aging roosters is related to increase testicular and plasma levels of estradiol. General and Comparative Endocrinology, 115, 23-28.
- Xu, Y., Li, J., Liang, W. & Zhu, W. (2013). Evaluation on Changes of Testicular Histology in Aging Men. Journal of Reproduction and Contraception, 24, 199-204.
- Zirkin, B. R. & Chen, H. (2000). Regulation of Leydig cell steroidogenic function during aging. Biology of Reproduction, 63, 977-81.
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