تأثیر کورکومین بر پروفیل لیپیدهای پلاسما و برخی فراسنجه‌های کیفیت اسپرم خروس‌های مادرگوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 استادیار بخش پاتولوژی و حیوانات تحت آزمایش، مؤسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران

5 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

6 مدیر تولید گروه تولیدی رامسر طیور، تنکابن، ایران

7 دانشیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

هدف این آزمایش، مطالعه اثر تغذیه کورکومین بر پروفیل لیپیدهای پلاسما و برخی از فراسنجه‌های اسپرم خروس‌های مادرگوشتی بود. آزمایش با 28 قطعه خروس سویه راس 308 از سن 51 هفتگی و به مدت 9 هفته، در قالب یک طرح کاملاً تصادفی با 4 تیمار و 7 تکرار، در قفس‌های انفرادی انجام شد. تیمارهای آزمایشی شامل سطوح مختلف کورکومین بودند (تیمار 1: فاقد کورکومین (شاهد)، تیمار 2: 006/0، تیمار 3: 012/0 و تیمار 4: 018/0 درصد جیره) که به جیره پایه افزوده شد. به‌منظور اندازه‌گیری پروفیل چربی‌های پلاسما، در پایان آزمایش از 5 قطعه خروس در هر گروه تیماری خون­گیری شد. همچنین، طی دوره آزمایش، نمونه‌های منی برای ارزیابی جنبایی و یکپارچگی غشای اسپرم به‌صورت هفتگی جمع‌آوری شد. غلظت گلوکز، تری‌گلیسرید، کلسترول‌ کل و لیپوپروتئین­های با چگالی پایین (LDL) پلاسما در تیمار 3 و 4 به‌طور معنی‌داری نسبت به گروه شاهد کاهش و غلظت لیپوپروتئین­های با چگالی بالا (HDL) افزایش یافت (05/0>P). بین تیمار 2 و گروه شاهد از نظر غلظت گلوکز و فراسنجه‌های چربی پلاسما تفاوت معنی‌داری وجود نداشت. یکپارچگی غشای پلاسمایی و جنبایی اسپرم در تیمارهای آزمایشی نسبت به گروه شاهد به­صورت خطی افزایش یافت (05/0>P). بین تیمارهای آزمایشی، تیمار 4 بیشترین تأثیر بر پروفیل لیپیدهای پلاسما و همچنین بیشترین میزان  بهبود جنبایی و یکپارچگی غشای اسپرم را داشت. در کل، با در نظر گرفتن همه شاخص­های اندازه‌گیری­شده، افزودن 018/0 درصد کورکومین به جیره پاسخ بهتری نسبت به دیگر تیمارها از خود نشان داد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of Curcumin on plasma lipid profile and some sperm quality traits in broiler breeder roosters

نویسندگان [English]

  • Amin Kazemi 1
  • Ahmad Zareh Shahneh 2
  • Saeed Zeinoaldini 3
  • Ali Reza Yousefi 4
  • Mehdi Heidari 5
  • Meysam Tavakoli-Alamooti 6
  • Zarbakhat Ansari 7
1 M.Sc. Student, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associated Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Assistant Professor, Department of Pathology and Experimental Animals, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5 Ph. D. Candidate, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
6 Production Manager, Ramsar Toyoor Production Group, Tonekabon, Iran
7 Associate Professor, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
چکیده [English]

This study was aimed to determine the effect of dietary Curcumin supplementation on the plasma lipid profile and some sperm quality parameters in broiler breeder roosters. In a completely randomized design, a total of twenty-eight 51-weeks-old Ross-308 roosters were randomly assigned to 4 treatment groups (n=7) and individually caged for 9 successive weeks. Treatments were different levels of Curcumin that were added to a basal diet including: T1, control (no Curcumin supplement), T2, 0.006%; T3, 0.012%, and T4, 0.018% of the diet. To determine plasma lipid profile, blood samples were collected from five birds/treatment at the end of the trial. Also, semen samples were weekly collected from each bird during the experiment, and sperm motility and plasma membrane integrity were evaluated. The results showed that concentrations of the plasma glucose, triglyceride, total cholesterol, and LDL were decreased, and concentrations of HDL were increased in T3 and T4 groups compared to the control group (P < 0.05). There were no significant differences in plasma lipid profile and plasma concentration of glucose between T1 and the control group (P<0.05). Sperm plasma membrane integrity and motility were linearly improved in treated groups compared to the control (P<0.05). The highest decrease in plasma lipid profile and most improvements in sperm motility and plasma membrane integrity was observed in T4 groups compared with other groups. In conclusion, considering all the measured parameters, dietary supplementation of 0.018% Curcumin had the best response on modifying plasma lipid profiles and improving sperm quality characteristics compared with other treatments.

کلیدواژه‌ها [English]

  • Curcumin
  • lipid metabolism
  • plasma
  • reproduction
  • Rooster

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علوم دامی ایران
دوره 50، شماره 1
خرداد 1398
صفحه 23-33

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  • تاریخ دریافت: 17 مرداد 1396
  • تاریخ بازنگری: 07 آبان 1396
  • تاریخ پذیرش: 07 آبان 1396

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