تأثیر زمان‌های گوناگون تزریق GnRH بر عملکرد تولیدمثلی میش‌های لک‌قشقایی سوپراوولاسیون‌شده با eCG در شرایط پرورش عشایری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد فیزیولوژی دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران

2 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه یاسوج، یاسوج، ایران

چکیده

به منظور بررسی تأثیر زمان‌های مختلف تزریق GnRH بر فعالیت‌های تولیدمثلی میش‌های لک‌قشقایی سوپراووله‌شده با eCG در فصل پاییز، 60 رأس میش (2 تا 3 ساله و میانگین وزنی 8/1±49 کیلوگرم) انتخاب شدند. چرخه فحلی میش‌ها با اسفنج‌های پروژسترون‌دار برای یک دوره 14 روزه هم‌زمان شد. یک روز قبل از خارج نمودن اسفنج‌ها، 400 واحد بین‌المللی eCG به صورت درون‌ماهیچه‌ای تزریق شد. سپس میش‌ها بر اساس عدم دریافت (شاهد) یا دریافت GnRH، در روزهای یک، هفت و 12 پس از فحلی به چهار گروه تقسیم شدند و طی دو چرخه فحلی پیاپی، شاخص‌های تولیدمثلی مانند نرخ بازگشت به فحلی و نرخ آبستنی اندازه‌گیری و بین گروه‌ها مقایسه شد. نتایج نشان داد بین گروه‌های مختلف آزمایشی از لحاظ نرخ بازگشت فحلی، نرخ آبستنی، میش‌های زایمان‌کرده، فکاندیتی و پرولیفیکیسی تفاوتی وجود نداشت (05/0<P)، ولی نرخ دوقلوزایی در میش‌هایی که در روزهای هفت و 12 پس از فحلی به آن‌ها GnRH تزریق شد، نسبت به گروهی که در روز نخست پس از فحلی GnRH دریافت کردند، کمتر بود (05/0>P). غلظت پروژسترون سرم در میش‌های آبستن، زایمان‌کرده، تک‌قلوزا و دوقلوزا که پس از فحلی به آن‌ها GnRH تزریق شد، بیشتر از میش‌های شاهد بود (05/0>P). به‌طور کلی استفاده از GnRH طی روزهای مختلف پس از جفت‌گیری در میش‌های لک‌قشقایی سبب افزایش غلظت پروژسترون گردید. علاوه بر این نرخ دوقلوزایی در میش‌های دریافت‌کننده GnRH در روز نخست نسبت روزهای هفتم و دوازدهم پس از فحلی به‌طور معنی‌داری بیشتر بود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of different times of GnRH injection on reproductive performance of Lake-Ghashghaei ewes supperovulated with eCG in nomadic breeding condition

نویسندگان [English]

  • Hossein Zahedpor 1
  • Javad Habibizad 2
  • Mehrdad Meamar 2
1 M. Sc. Student, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
2 Assistant Professor, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
چکیده [English]

In order to investigate the different times of GnRH injection on the reproductive activities of Lake-Ghashghaei ewes supperovulated with eCG in autumn season, 60 ewes (2-3 ages and average body weight 49±1.8 Kg) were chosen. Estrus cycle of ewes were synchronized by progesterone sponges during a 14 days period. One day before removing the sponges, 400 IU of eCG were injected intramuscularly. Then the ewes were divided into 4 groups according to receiving GnRH on different days (one, seven and 12 days) or not receiving GnRH (control) after estrus observation and reproductive indexes such as the rate of return to estrus and pregnancy rate, were measured and compared among different groups in two continuous estrus cycle. The result indicated that there were not any significant differences (P>0.05) in the rate of return to estrus, pregnancy rate, parturation rate, fecundity and prolificacy among treatment groups, but the rate of twin birth was significantly (P<0.05) lesser in the groups, receiving GnRH in 7 and 12 days compared with the group that recieved GnRH in the first day. Serum progesterone concentration in pregnant, delivered, single and twin births ewes that received GnRH were significantly (P<0.05) more than control ewes. In conclusion, GnRH administration during different days after mating increased serum progesterone concentration in Lake-Ghashghaei ewes. Moreover, the rate of twining birth in ewes which received GnRH on the first day, was significantly more compared with using GnRH on 7th and 12th days after estrus.

کلیدواژه‌ها [English]

  • Equine Chorionic Gonadotropin
  • Gonadotropin Releasing Hormone
  • pregnancy rate
  • Lake-Ghashghaei ewes
  • twining rate

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علوم دامی ایران
دوره 49، شماره 4
اسفند 1397
صفحه 557-565

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سابقه مقاله

  • تاریخ دریافت: 28 شهریور 1397
  • تاریخ بازنگری: 11 اسفند 1397
  • تاریخ پذیرش: 20 اسفند 1397

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