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تأثیر تزریق داخل تخم مرغی نانوذرات عصاره هسته انگور بر تمایز جنسیت و ساختار گونادی در جوجه‌های گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار شیمی آلی، مجتمع فناوری‌های پیشرفته پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران

چکیده

پلی­فنول­های موجود در هسته انگور جز مهارکننده­های قوی آروماتاز محسوب می­شوند. در این مطالعه، عصاره هسته انگور به عنوان آنتی­آروماتاز به تخم­مرغ تزریق و اثرات آن بر تمایز جنسیت و ساختار گوناد جوجه­ها مورد بررسی قرار گرفت. هفتصد عدد تخم­مرغ از سویه راس 308 به 7 تیمار شامل: تزریق سطوح 3 (GSE3+Zn) و 4 میلی­گرم (GSE4+Zn) عصاره هسته انگور و روی، 3(NGSE3+Zn) و 4(NGSE4+Zn) میلی­گرم نانوذرات عصاره هسته انگور و روی، 05/0 میلی­گرم تاموکسیفن و روی (TOM+Zn)، 5/0 میلی­لیتر نرمال سالین (PC) و تیمار بدون تزریق (NC)، اختصاص یافتند. تزریق در روز 5 انکوباسیون و از قسمت باریک تخم­مرغ انجام شد. جوجه­های تولیدی به مدت 5 هفته پرورش یافتند. نتایج به‌دست‌آمده نشان داد که در مقایسه با تیمارهای شاهد تزریق عصاره هسته انگور باعث افزایش معنی­دار در درصد جنس نر شد (05/0P<). مقایسه تیمارهای مختلف حاکی از عدم تفاوت معنی‌داری در وزن یک روزگی و نیز صفات عملکردی (خوراک مصرفی، وزن بدن، افزایش وزن روزانه و ضریب تبدیل خوراک) بود. مطالعه ساختار گوناد در جوجه­ها، حاکی از درجه قوی برگشتگی جنسی در جوجه­های تیمار شده با سطوح 3(NGSE3) و 4(NGSE4) میلی­گرم نانوذرات عصاره هسته انگور و روی بود. نتیجه­گیری کلی حاکی از این است که عصاره هسته انگور به عنوان ترکیبی با خاصیت آنتی آروماتازی، قابلیت تولید درصد بیشتر جنس نر جوجه­های گوشتی را دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of in-ovo injection of nanoparticles of grape seed extract on sex differentiation and gonadal structure in broiler chicks

نویسندگان [English]

  • Zeinab Shohoudi 1
  • Majid Mottaghitalab 2
  • Mostafa Golshekan 3
1 Former M.Sc. Student and Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 Assistant Professor of Organic Chemistry,Institute of Medical Advanced Technologies , Guilan University of Medical Sciences, Rasht, Iran
چکیده [English]

Polyphenols content in the grape seed are considered as potent aromatase inhibitors. In this study, the grape seed extract was injected into the egg as an anti-aromatase and its effects on sex differentiation and gonadal structure in broiler chicks were investigated. A total of seven hundred eggs from Ross 308 were divided into 7 experiment groups.  Treatments including: injection of 3(GSE3) and 4(GSE4) milligrams of grape seed extract and zinc, 3 (NGSE3) and 4(NGSE4) milligrams of nanoparticle of grape seed extract and zinc, 0.05 mg tamoxifen and zinc (TOM+Zn), 0.5 ml normal saline (PC) and control with no injection (NC). Injection was performed on day 5 of incubation from the narrow side of egg. After hatch, chickens were reared for 5 weeks. Results showed that compared to controls, injection of grape seed extract caused significant increase in percentage of male chick(P<0.05). No significant differences were found in day old chick weights (P>0.05). Feed intake, body weight, body weight gain and feed conversion ratio appeared with no significant differences (P>0.05). The study of gonadal structure in chicks indicated a high degree of sexual reversal in chicken treated with 3(NGSE3) and 4(NGSE4) mg of nanoparticles of grape seed extract and zinc. Conclusion is that the grape seed extract has the potential to act as an anti-aromatase compound to produce higher percentage of male broiler chicks.

کلیدواژه‌ها [English]

  • Anti aromatase
  • broiler chicks
  • In-Ovo injection
  • sex reversal
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