تأثیر تزریق داخل تخم مرغی نانوذرات عصاره هسته انگور بر تمایز جنسیت و ساختار گونادی در جوجه‌های گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار شیمی آلی، مجتمع فناوری‌های پیشرفته پزشکی، دانشگاه علوم پزشکی گیلان، رشت، ایران

چکیده

پلی­فنول­های موجود در هسته انگور جز مهارکننده­های قوی آروماتاز محسوب می­شوند. در این مطالعه، عصاره هسته انگور به عنوان آنتی­آروماتاز به تخم­مرغ تزریق و اثرات آن بر تمایز جنسیت و ساختار گوناد جوجه­ها مورد بررسی قرار گرفت. هفتصد عدد تخم­مرغ از سویه راس 308 به 7 تیمار شامل: تزریق سطوح 3 (GSE3+Zn) و 4 میلی­گرم (GSE4+Zn) عصاره هسته انگور و روی، 3(NGSE3+Zn) و 4(NGSE4+Zn) میلی­گرم نانوذرات عصاره هسته انگور و روی، 05/0 میلی­گرم تاموکسیفن و روی (TOM+Zn)، 5/0 میلی­لیتر نرمال سالین (PC) و تیمار بدون تزریق (NC)، اختصاص یافتند. تزریق در روز 5 انکوباسیون و از قسمت باریک تخم­مرغ انجام شد. جوجه­های تولیدی به مدت 5 هفته پرورش یافتند. نتایج به‌دست‌آمده نشان داد که در مقایسه با تیمارهای شاهد تزریق عصاره هسته انگور باعث افزایش معنی­دار در درصد جنس نر شد (05/0P<). مقایسه تیمارهای مختلف حاکی از عدم تفاوت معنی‌داری در وزن یک روزگی و نیز صفات عملکردی (خوراک مصرفی، وزن بدن، افزایش وزن روزانه و ضریب تبدیل خوراک) بود. مطالعه ساختار گوناد در جوجه­ها، حاکی از درجه قوی برگشتگی جنسی در جوجه­های تیمار شده با سطوح 3(NGSE3) و 4(NGSE4) میلی­گرم نانوذرات عصاره هسته انگور و روی بود. نتیجه­گیری کلی حاکی از این است که عصاره هسته انگور به عنوان ترکیبی با خاصیت آنتی آروماتازی، قابلیت تولید درصد بیشتر جنس نر جوجه­های گوشتی را دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of in-ovo injection of nanoparticles of grape seed extract on sex differentiation and gonadal structure in broiler chicks

نویسندگان [English]

  • Zeinab Shohoudi 1
  • Majid Mottaghitalab 2
  • Mostafa Golshekan 3
1 Former M.Sc. Student and Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 Assistant Professor of Organic Chemistry,Institute of Medical Advanced Technologies , Guilan University of Medical Sciences, Rasht, Iran
چکیده [English]

Polyphenols content in the grape seed are considered as potent aromatase inhibitors. In this study, the grape seed extract was injected into the egg as an anti-aromatase and its effects on sex differentiation and gonadal structure in broiler chicks were investigated. A total of seven hundred eggs from Ross 308 were divided into 7 experiment groups.  Treatments including: injection of 3(GSE3) and 4(GSE4) milligrams of grape seed extract and zinc, 3 (NGSE3) and 4(NGSE4) milligrams of nanoparticle of grape seed extract and zinc, 0.05 mg tamoxifen and zinc (TOM+Zn), 0.5 ml normal saline (PC) and control with no injection (NC). Injection was performed on day 5 of incubation from the narrow side of egg. After hatch, chickens were reared for 5 weeks. Results showed that compared to controls, injection of grape seed extract caused significant increase in percentage of male chick(P<0.05). No significant differences were found in day old chick weights (P>0.05). Feed intake, body weight, body weight gain and feed conversion ratio appeared with no significant differences (P>0.05). The study of gonadal structure in chicks indicated a high degree of sexual reversal in chicken treated with 3(NGSE3) and 4(NGSE4) mg of nanoparticles of grape seed extract and zinc. Conclusion is that the grape seed extract has the potential to act as an anti-aromatase compound to produce higher percentage of male broiler chicks.

کلیدواژه‌ها [English]

  • Anti aromatase
  • broiler chicks
  • In-Ovo injection
  • sex reversal

مراجع

  1. Abinawanto, K., Shimada, K., Yoshida, V. & Saito, N. (1996). Effects of Aromatase Inhibitor on Sex Differentiation and Levels of p45017a and p450arom Messenger Ribonucleic Acid of Gonads in Chicken Emryos. General and Comparative Endocrinology, 102, 241-246.
  2. Arnold, A. P. & Itoh, Y. (2011). Factors Causing Sex Differences in Birds. Avian Biology Research, 4(2).
  3. Aslani, M. (2011). The effect of black tea extracts on sexual differentiation of broiler chicks. Master's Thesis. Faculty of Agricultural Sciences, University of Guilan. (in Farsi)
  4. Ayers, K. L., Davidson, N. M., Demiyah, D., Roeszler, K. N. & Grutzner, F. (2013). RNA Sequencing Reveals Sexually Dimorphic Gene Expression before Gonadal Differentiation in Chicken and Allows Comprehensive Annotation of the W Chromosome. Genome Biology, 14, 26.
  5. Brenes, A., Viveros, A., Gon, I., Centeno, C., Sayago-Ayerdy, S. G., Arija, I. & Saura-Calixto, F. (2008). Effect of Grape Pomace Concentrate and Vitamin E on Digestibility of Polyphenols and Antioxidant Activity in Chickens. Poultry Science, 87, 307-316.
  6. Burke, W. & Henry, M. (1999). Gonadal Development and Growth of Chickens and Turkeys Hatched from Eggs Injected with an Aromatase Inhibitor. Poultry Science, 78, 1019-1033.
  7. Burstein, H. J., Temin, S., Anderson, H., Buchholz, T. A., Davidson, N. E. & Gelmon, K. E. (2014). Adjuvant Endocrine Therapy for Women with Hormone Receptor-Positive Breast Cancer: American Society of Clinical Oncology Clinical Practice Guideline Focused Update. American Society of Clinical Oncology, 32(21), 2255-2269. 
  8. Dowsett, M., Forbes, J. F., Bradley, R., Ingle, J., Aihara, T. & Bliss, J. (2015). Aromatase Inhibitors versus Tamoxifen in Early Breast Cancer: Patient-Level Meta-Analysis of the Randomised Trials. American Society of Clinical Oncology, 386(10001), 1341-1352.
  9. Durmus, I., Atasoglu, C., Mizrak, C., Ertas, S. & Kaya, M. (2004). Effect of increasing zinc concentration in the diets of brown parent stock layers on various production and hatchability traits. Arch. Tierz. Dummerstorf, 47(5), 483-489.
  10. Ellegren, H. (2002). Evolution of the Avian Sex Chromosomes and Their Role in Sex Determination. Trends in Ecology and Evolution, 15, 188-192.
  11. Fazli, N., Hassanabadi, A., Mottaghitalab, M. & Hajati, H. (2015). Manipulation of Broiler Chickens Sex Differetiation by In Ovo Injection of Aromatase Inhibitors, and Garlic and Tomato Extracts. Poultry Science, 94, 2778-2783.
  12. Hudson Q. J., Smith, C. A. & Sinclair, A. H. (2005). Aromatase Inhibition Reduces Expression of FOXL2 in the Embryonic Chicken Ovary. Dev Dyn, 233, 1052-1055.
  13. Kijima, L., Phung, S., Hur, G. & Kwok, S. (2006). Grape Seed Extract Is an Aromatase Inhibitor and a Suppressor of Aromatase Expression. Cancer Res, 66(11), 5960-5967.
  14. Mafi, M. (2010). Manipulation of the sexual difference of broiler chickens using in-ovo injection of black noodle extracts. M.Sc. thesis. Faculty of Agricultural Sciences, University of Guilan. (in Farsi)
  15. Mohseni Kouchesfahani, H., Parivar, K. & Salmabadi, Z. (2015).  Effect of Hydroalcoholic Grape Seed Extract (Vitis vinifera L.) on Polycystic Ovarian Syndrome in Female Wistar Rat. Journal of Cell & Tissue, 6(2), 143-151. (in Farsi)
  16. Mottaghitalab, M. & Valizade, E. (2002). Garlic Extraction and Aromatase Interaction on Sex Differension in Chicks. In: Proceedings of WPSA Spring Meeting. York, UK. 9-10 April.
  17. Mottaghitalab, M., Fazli, N. & Hassan Abadi, A. (2009). In-Ovo Injection Technology: Effects of Different Aromatase Inhibitors on Sex Differentiation. Journal of the Iranian Chemical Society, 6(1), 20-72.
  18. Salata, O. V. (2004). Applications of Nanoparticles in Biology and Medicine. Journal of Nano Biotechnology, 2, 3.
  19. Schieber, A., Stintzing, F. C. & Carle, R. (2002). By-Products of Plant Food Processing as a Source of Functional Compounds-Recent Developments. Trends Food Science. Technol, 12, 401-413.
  20. Simpson, E., Micheal, D. M., Agarwal, V. R., Hinshelwood, M. M., Bulun, S. E. & Zhao, Y. (2001). Cytochromes p450 11: Expression of the CYP19 (Aromatase) Gene: An Unusuall Case of Alternative Promoter Usage. Tissue cell.
  21. Vaillant, S., Dorizzi, M., Pieau, C. & Richard, N. (2001). Sex Reversal and Aromatase in Chicken. Journal of Experimental Zoology, 290, 727-740.  
  22. Villapando, I., Sanchez, G., Sanchez, I., Pedernera, E. & Villafan, H. (2000). The P450 Aromatase Gene is Asymmetrically Expressed in a Critical Period for Gonadal Sexual Differentiation in the Chick. Gen Comp Endocrinol, 117, 325-334.
  23. Wang, M. L., Suo, X., Gu, J. H., Zhang, W. W., Fang, Q. & Wang, X. (2008). Influence of Grape Seed Proanthocyanidin Extract in Broiler Chickens: Effect on Chicken Coccidiosis and Antioxidant Status. Poultry Science Association Inc, 2273-2280.
  24. Wartenberg, H., Lenz, H. & Schweikert, H. U. (1992). Sexual Differentiation and the Germ Cell in Sex Reversed Gonads after Aromatase Inhibition in the Chicken Embryo. Journal of Poultry Science, 24(1), 1-6.  
  25. Wong, D., Villanueva, J., Cress, A. & Duleba, A. (2010). Effects of Resveratrol on Proliferationand Apoptosis in Rat Ovarian Theca-Interstitial Cells. Molecular Human Reproduction, 16(4), 251-259.
  26. Zhang, S. O., Mathur, S., Hattem, G., Tassy, O. & Pourquie, O. (2010). Sex-Dimorphic Gene Expression and Ineffective Dosage Compensation of Z-Linked Genes in Gastrulating Chicken Embryos. BMC Genomics, 11, 13.
علوم دامی ایران
دوره 49، شماره 4
اسفند 1397
صفحه 495-504

فایل ها

سابقه مقاله

  • تاریخ دریافت: 14 مهر 1397
  • تاریخ بازنگری: 23 دی 1397
  • تاریخ پذیرش: 23 دی 1397

هم رسانی

ارجاع به این مقاله

آمار