اثر منابع مختلف کربوهیدات بر بازدهی استفاده از خیساب مایع ذرت در جیره غذایی در شرایط برون‌تنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تغذیه دام، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان

2 دانشیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان

3 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه لرستان

چکیده

هدف از این تحقیق، بررسی اثر مکمل نمودن خیساب مایع ذرت به عنوان منبع نیتروژن با منابع مختلف کربوهیدرات در جیره غذایی بر تولید گاز، فراسنجه‌های تخمیر، گوارش‌پذیری مواد مغذی و فعالیت آنزیم‌های میکروبی شکمبه در شرایط برون‌تنی بود. از هشت جیره غذایی حاوی منابع مختلف کربوهیدراته (شامل سطوح مخلتف جو، ذرت و ملاس) که در همه آن‌ها از یک سطح ثابت خیساب مایع ذرت در نظر گرفته شده بود (13 درصد ماده خشک)، به عنوان سوبسترای انکوباسیون آزمایشگاهی استفاده گردید. جیره‌های آزمایشی شامل جیره حاوی 1) ذرت، 2) جو، 3) مخلوط جو و ذرت و جایگزینی جیره حاوی مخلوط جو و ذرت به ترتیب با سطوح 4) 5، 5) 10، 6) 15، 7) 20 و 8) 25 درصد ملاس بودند. بیشترین و کمترین میزان تولید گاز در زمان 72 و 96 ساعت انکوباسیون و پتانسیل (b) تولید گاز به ترتیب با انکوباسیون جیره حاوی 15 درصد ملاس و جیره حاوی ذرت به­دست آمد (05/0P<). هرچند، در مورد نرخ تولید گاز (c) و سنتز پروتئین میکروبی بیشترین و کمترین میزان به ترتیب در جیره حاوی 10 و 25 درصد ملاس مشاهده گردید (05/0P<). بیشترین میزان ناپدید شدن ماده خشک شکمبه‌ای با مکمل نمودن خیساب ذرت با سطح 10 درصد ملاس و کمترین میزان آن با انکوباسیون جیره حاوی ذرت به­دست آمد (05/0>P). بیشترین میزان غلظت نیتروژن آمونیاکی با مکمل نمودن خیساب ذرت با سطح 25 درصد ملاس به­دست آمد، اما کمترین میزان آن در جیره حاوی 10 درصد ملاس مشاهده گردید (05/0P<). مکمل نمودن خیساب مایع ذرت با سطح 10 درصد ملاس سبب افزایش فعالیت آنزیم‌های کربوکسی متیل سلولاز و میکروکریستالین سلولاز در مقایسه با سایر تیمارهای آزمایشی شد (05/0>P). بیشترین میزان فعالیت تجزیه کاغذ صافی در تیمار حاوی خیساب ذرت مکمل شده با سطح 5 درصد ملاس و کمترین میزان آن در تیمار حاوری 25 درصد ملاس حاصل گردید (05/0>P). در کل، نتایج نشان داد که مکمل نمودن جیره غذایی خیساب ذرت مایع با سطح 10 درصد ملاس سبب بهبود هضم و تخمیر و متابولیسم نیتروژن در شرایط برون‌تنی شد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of different dietary carbohydrate sources on in vitro utilization efficiency of corn steep liquor

نویسندگان [English]

  • Parmis Zahedi Moghadam 1
  • Arash Azarfar 2
  • Ayoub Azizi 3
1 Ph.D. Candidate, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran
3 Assistant Professor, Department of Animal Science, Faculty of Agricuture, Lorestan University, Khorramabad, Iran
چکیده [English]

This study was carried out to evaluate the effects of supplementing corn steep liquor (CSL), as a nitrogen source, with different dietary carbohydrate sources on in vitro gas production, fermentation parameters, digestibility and activity of rumen microbial enzymes of male lamb's diets. The 8 experimental diets contained different energy sources (including different levels of barley, corn and molasses), in which a constant level of CSL was used (13% of diet dry matter (DM)), incubated in vitro. Experimental diets were 1) corn, 2) barley, 3) mixture of corn/barley and replacing the former with4) 5, 5) 10, 6) 15, 7) 20 and 8) 25% molasses. The maximum and minimum gas production at 72 and 96 (h) of incubation, and potential of gas production (b) were obtained by incubation of diet containing15% molasses and corn diets, respectively (P<0.05). However, the greatest and lowest values for fractional rate of gas production (c) and microbial protein synthesiswere observed in the diets containing 10 and 25% molasses, respectively (P<0.05). The concentration of NH3-N was greatest by supplementing CSL with 25% molasses, while it was lowest in the diet containing 10% molasses (P<0.05). The addition 10% molasses along with CSL increased the activity of carboxymethyle cellulase and microcrystalline cellulase compared with the other treatments (P<0.05). The highest activity of filter paper-degrading (FDP) was obsereved with the diet in which CLS was supplemented with 5% molasses, while supplementing CSL conating diet with 25% molasses led to the lowest FPD activity (P<0.05). In conclusion, the results of present study showed that supplementing CSL containing diet with 10% of molasses improved nutrient digestion, rumen fermentation and nitrogen metabolism in vitro.

کلیدواژه‌ها [English]

  • Corn steep liquor
  • digestibility
  • Enzyme activity
  • Fermentation
  • grains
  • molasses

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علوم دامی ایران
دوره 49، شماره 4
اسفند 1397
صفحه 469-479

فایل ها

سابقه مقاله

  • تاریخ دریافت: 05 آبان 1397
  • تاریخ بازنگری: 29 آذر 1397
  • تاریخ پذیرش: 17 دی 1397

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