اثر چرخه تخمدانی بر بیان گیرنده‌های زنگوله‌ای شکل TLR4 در آمپولا و ایستموس اویداکت گاوهای شیری هلشتاین

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان

2 دانشجوی دکتری، گروه علوم دامی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان

چکیده

هدف از مطالعه حاضر، بررسی الگوی بیان ژن TLR4 در آمپولا و ایستموس اویداکت گاو در مراحل مختلف چرخه تخمدانی (پیش از تخمک­ریزی، پس از تخمک­ریزی و میانه چرخه) بود. مرحله چرخه تخمدانی با توجه به ظاهر، اندازه و رنگ جسم زرد و فولیکول چیره مشخص شد. بیان ژن TLR4 در ایستموس و آمپولای 40 اویداکت به­روش qRT-PCR اندازه­گیری شد. نتایج نشان داد، که در اویداکت­های واقع در سمت تخمدان فعال (دارای فولیکول غالب یا جسم زرد)، میزان بیان ژن TLR4 در ایستموس به­طور معنی­داری بیش­تر از آمپولا بود. همچنین، پیش از تخمک­ریزی، بیان ژن TLR4 در آمپولا و ایستموس اویداکت­های واقع در سمت تخمدان فعال به‌صورت معنی­داری از دیگر مراحل چرخه تخمدانی و همچنین از آمپولا و ایستموس اویداکت­های مربوط به تخمدان غیرفعال (فاقد فولیکول غالب یا جسم زرد) بیشتر بود. به­طورکلی نتایج این مطالعه نشان داد که بیان ژن TLR4 در اویداکت گاو تحت تأثیر وضعیت هورمونی (چرخه ­تخمدانی)، موقعیت قرارگیری آن نسبت به تخمدان فعال و ناحیه اویداکتی (آمپولا یا ایستموس) قرار دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of ovarian cycle on TLR4 mRNA expression pattern in ampulla and isthmus of the bovine oviduct

نویسندگان [English]

  • Rasoul Kowsar 1
  • Morteza Zahrazadeh 2
  • Ali Sadeghi Sefidmazgi 1
1 Assistant Professor, Department of Animal Sciences, Faculty of Agriculture, Isfahan University of Technology, PO BOX: 84156/83111, Isfahan, Iran
2 Ph. D. Candidate, Department of Animal Sciences, Faculty of Agriculture, Isfahan University of Technology, PO BOX: 84156/83111, Isfahan, Iran
چکیده [English]

This study was conducted to evaluate the TLR4 gene expression's pattern in ampulla and isthmus of dairy cows during the ovarian cycle. The ovarian cycle phase was determined based on the appearance, size, and color of corpus luteum and dominant follicle. The TLR4 expression was assessed in ampulla and isthmus of 40 oviducts using qRT-PCR. Results showed at the pre-ovulatory phase, TLR4 gene expression in the isthmus of ipsilateral oviducts was significantly greater than that in ampulla. Also, the expression of TLR4 in ampulla and isthmus of the oviducts ipsilateral to the pre-ovulatory ovary was significantly greater than that in the ampulla and isthmus of oviducts contralateral to the post-ovulatory or mid-cycle ovaries. In general, the present study showed that, TLR4 mRNA expression was differentially influenced by the stage of the ovarian cycle, its relation to the functional ovary, and the oviductal parts (ampulla or isthmus) in bovine.

کلیدواژه‌ها [English]

  • Dairy cow
  • estrous cycle
  • innate immunity
  • oviduct
  • TLR4

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علوم دامی ایران
دوره 49، شماره 3
آذر 1397
صفحه 371-379

فایل ها

سابقه مقاله

  • تاریخ دریافت: 23 خرداد 1397
  • تاریخ بازنگری: 14 شهریور 1397
  • تاریخ پذیرش: 24 شهریور 1397

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