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تعدیل تأثیر تنش القایی با تزریق کورتیکوسترون با استفاده از پروبیوتیک در جوجه‌های گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تغذیۀ دام، دانشکدۀ کشاورزی دانشگاه گیلان، رشت

2 دانشیار، دانشکدۀ کشاورزی دانشگاه گیلان، رشت

3 دانشیار، گروه بیوشیمی دانشکدۀ علوم زیستی، دانشگاه تربیت مدرس تهران

چکیده

این آزمایش در قالب طرح کامل تصادفی با آرایش فاکتوریل 2×3 با دو سطح تزریق کورتیکوسترون (تزریق روغن و تزریق کورتیکوسترون) و سه سطح مصرف پروبیوتیک (1- بدون کاربرد پروبیوتیک، 2 و 3- به ترتیب 105×8/0 و 105×6/1 عدد از اسپور باسیلوس سوبتلیس در هر گرم خوراک) به منظور تعدیل تأثیر تنش القایی تزریق کورتیکوسترون با استفاده از پروبیوتیک در جوجه­های گوشتی انجام شد. هر تیمار در چهار تکرار و در هر تکرار دوازده قطعه جوجه استفاده شد. در سنین 7 تا 9 و نیز 25 تا 27 روزگی دورۀ پرورش تزریق زیرپوستی روغن ذرت با و بدون 2 میلی­گرم کورتیکوسترون به ازای هر کیلوگرم وزن بدن، دو بار در روز انجام شد. تزریق کورتیکوسترون موجب کاهش وزن نسبی و طول رودۀ کوچک در سنین 28 و 42 روزگی شد (05/0P<). وزن نسبی سینه در گروه‌های تحت تزریق کورتیکوسترون نسبت به گروه تزریق روغن کاهش نشان داد (05/0P<). طول پرزهای قسمت‌های مختلف روده و  عیار پادتن علیه واکسن نیوکاسل با تزریق کورتیکوسترون کاهش معنی‌دار یافت (05/0P<). افزودن پروبیوتیک به جیره، طول پرز در قسمت‌های مختلف روده و نیز وزن نسبی روده را افزایش داد (05/0P<). بازدۀ استفاده از خوراک و میانگین افزایش وزن روزانه در گروه­های مصرف‌کنندۀ پروبیوتیک افزایش پیدا کرد (05/0P<). جمعیت باکتری­های کلی‌فرم و لاکتوباسیلوس ایلئوم توسط تزریق کورتیکوسترون تحت تأثیر قرار نگرفت، اما افزودن پروبیوتیک بر آن تأثیر معنی­دار داشت (05/0P<). درمجموع نتایج این تحقیق دلالت بر اثرگذاری مثبت پروبیوتیک روی عملکرد و اندازۀ پرزهای رودۀ جوجه­های گوشتی تحت تنش القاء شده با تزریق کورتیکوسترون دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Modulating the effects of stress induced by corticosterone injection using probiotic administration in broiler chicks

نویسندگان [English]

  • Fakhreddin Abeddargahi 1
  • Hassan Darmani Kuhi 2
  • Reza Hassan Sajedi 3
  • Mohammad Roostaei Ali Mehr 2
1 Ph. D. Candidate, Department of Animal science, Faculty of Agriculture, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Animal science, Faculty of Agriculture, University of Guilan, Rasht, Iran
3 Associate Professor, Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
چکیده [English]

This experiment was conducted in a completely randomized design with 2×3 factorial arrangement with two levels of corticosterone injections (oil injection and corticosterone injections) and three levels of probiotic supplementation (1- without probiotic, 2 and 3- supplemented with 0.8×105 and 1.6×105 spores of Bacillus subtilis per gram of feed, respectively) to modulate stress induced by corticosterone injection using probiotic admimistration in broiler chicks. Each of 6 treatments replicated 4 times of 12birds per replicate.At 7 to 9 and 25 to 27 days of age, the chicks received one of the subcutaneous injections corn oil with/without CORT at 2 mg/kg BW twice per day. Corticosterone injection decreased the relative weight of intestine and its length at 28 and 42 days of ages (P<0.05). Relative weight of the breast was lower in corticosterone injected groups than in the oil injection groups (P<0.05). Villi height of different parts of the intestine and antibody levels against Newcastle disease decreased significantly with corticosterone injection.Adding probiotics increased the length of the villi and relative weight of the intestine. Feed to gain efficiency and average daily weight gain increased significantly in response to probiotic supplementation (P<0.05). Total coliforms and lactobacillus populations were not affected by corticosterone injections, but adding probiotic had a significant effect on ileal populations of these bacteria (P<0.05). Overall, the results of this study indicate a positive effect of probiotic on performance and intestine structure of broiler chicks under stress induced by corticosterone injection.

کلیدواژه‌ها [English]

  • Broiler Chickens
  • corticosterone
  • Probiotics
  • Stress
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