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بررسی مولکولی ژن‌های ‌مقاومت پادزیستی در جدایه‌های اشریشیا‌کلی در طیور گوشتی شهرستان شهربابک به روش MULTIPLEX PCR

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه میکرو‌بیولوژی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

2 استاد بخش ایمنی‌شناسی و میکرو‌بیولوژی دانشکدۀ دامپزشکی، دانشگاه تهران، تهران، ایران

3 استاد، گروه میکرو‌بیولوژی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران

چکیده

اشریشیاکلی بیماری‌زای طیور عامل ایجاد طیف بسیار گسترده‌ای از بیماری‌های خارج روده‌ای ازجمله سلولیت و کلی باسیلوز است. مشکل مقاومت به پادزیست (آنتی‌بیوتیک)‌ها در سرتاسر جهان وجود دارد. هدف از این پژوهش ارزیابی مولکولی ژن‌های مقاومت پادزیستی مانند: aac، aad، qnr، tet، anr، sul و تعیین حساسیت پادزیستی بود. نمونه‌برداری از کشتارگاه صنعتی طیور شهربابک در 6 ماه سال 95 انجام و شمار 83 جدایه از نمونه‌های کلی باسیلوزی و 34 جدایه از نمونه‌های سلولیتی جدا و به روش‌های بیوشیمیایی تأیید شدند. سپس آزمون‌های MultiplexPCR و تست حساسیت پادزیستی روی نمونه‌ها انجام شد. نتایج بررسی نشان داد، فراوانی ژن‌های tetA 85/63%، tetB65/62%، qnrs 3/49%،  09/24%،  91/28%،  32/31%،  5/26%، 1- aac(3)66/25%،  89/22%، flor 91/28%،  31/33% و  30/12درصد بود. هیچ‌کدام از نمونهها از نظر ژن‌های blaTEM  مثبت نبودند. در این بررسی مشخص شد که 40 تا 90 درصد همۀ جدایه‌ها به یک یا چند پادزیست مقاوم هستند. بیشترین مقاومت به ترتیب نسبت به پادزیست‌های تتراسایکلین، سولفومتوکسازول، کلرامفنیکل و تریمتوپریم بود. همچنین مشخص شد که بروز مقاومت پادزیستی به دلیل حضور ژن‌های مقاومت است. شناخت الگوی مقاومت و حساسیت ریزجانداران (میکروارگانیسم‌)ها نسبت به پادزیست‌ها در انتخاب مناسب و درست دارو و مهار (کنترل) عفونت‌ها نقش مؤثری دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Molecular analysis of antibiotic resistance genes in Escherichia coli from broiler chickens in shahrebabak by Multiplex PCR Technique

نویسندگان [English]

  • Akbar Asadi 1
  • Taghi Zahraei Salehi 2
  • Mahmoud Jamshidiyan 3
1 Ph. D. Candidate, Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Professor, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Professor, Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Avian pathogenic Escherichia coli (APEC) is responsible for an extremely wide range of extra intestinal diseases in poultry, including Colibacillosis and Cellulitis. There is the problem of antibiotic resistance all over the world. The aim of this study is the molecular study of antibiotic resistance genes, such as: aac, aad, qnr, tet, anr, sul and determination of antibiotic susceptibility. 83 strains of E. coli of Colibacillosis cases and 34 strains of Cellulitisin cases were gotten from in poultry slaughterhouses in Shahrebabak province within 6 months in 2017. Strains of E. coli were confirmed by biochemical methods. The results of the study showed that 63.85% for the tetA gene, 62.65% for the tetB gene were positive. The abundance of genes qnrs (49.3%), qnrB (24.09%), sul1 (28.91%), dhfrv (31.32%), dhfrA (26.50%), aac (3)-1(25.66%), aadA (22.89%), floR (28.91%), sul1 (33.13%), qnrA (12.30%). None of the specimens was positive for blaoxa, blaCTX, blasHV, blaTEM, genes. In this study, it was found that all of the suppositories were resist ant to one or more antibiotics between 40% and 90%. The highest resistance to antibiotics was tetracycline, sulfamethoxazole, chloramphenicol, terimetoprim. In this study, antibiotic resistance was found to be due to the presence of resistance genes. Recognition of resistance pattern and microorganisms susceptibility to antibiotics have an effective role on correct and suitable selection of antibiotic and infection control.

کلیدواژه‌ها [English]

  • Antibiotic resistance
  • Broiler Chicken
  • celluitis
  • collibacillosis
  • Escherichia coli
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