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تأثیر مکمل بتائین و تزریق ویتامین B12 در دورۀ انتقال بر فراسنجه‌های سرم خون در گاوهای شیری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشیار، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

2 استادیار، گروه علوم دامی، دانشکده کشاورزی، دانشگاه ایلام، ایران

3 دانشجوی دکتری، دانشکده کشاورزی، دانشگاه لرستان، خرم آباد، ایران

چکیده

هدف از این تحقیق ارزیابی تأثیر مکمل بتائین و تزریق ویتامین B12 در دورۀ انتقال روی فراسنجه‌های سرم گاوهای شیری در دورۀ انتقال است. بدین منظور 32 گاو هلشتاین چند بار زایش کرده (21 روز پیشپیش از زایش) انتخاب شدند و به یکی از چهار تیمار آزمایشی تخصیص داده شدند: تیمار شاهد یا CC (بدون تزریق ویتامین B12 و دریافت مکمل بتائین)، تیمار دوم یا  CB(تزریق هفتگی 10 سی‌سی ویتامین B12)، تیمار سوم یا BC (دریافت روزانه 50 گرم مکمل بتائین) و تیمار چهارم یا BB (تزریق هفتگی 10 سی‌سی ویتامینB12و دریافت روزانه 50 گرم مکمل بتائین). نمونه­های خون در روزهای 7 روز پیش از زایش و همچنین 2 و 42 روز پس از زایش برای تعیین فراسنجه­های خونی سرم گاوها و همچنین خون گوساله­های تازه متولدشده گرفته شد. فراسنجه­های سرم خونی گاوها تحت تأثیر تیمارهای آزمایشی قرار نگرفتند (05/0P>)، به‌جز برای گلوکز که در تیمارهای بتائین و B12 + بتائین نسبت به دو تیمار دیگر به‌طور معنی‌داری بیشتر بود (05/0P<). در بین شاخص‌های آنزیمی، گاماگلوتامیل ترانسفراز در تیمار B12 + بتائین در مقایسه با تیمار دوم (B12) کاهش معنی­دار داشت (05/0P<). فراسنجه‌های سرمی گوساله‌های تازه متولدشده از گاوهای دورۀ انتقال تحت تأثیر تیمارها قرار نگرفت. در نتیجه بتائین و ویتامین B12 تنها توانستند در بین فراسنجه‌های خونی گلوکز را افزایش دهند.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of betaine supplementation and B12 injection during the transition period on serum metabolite in dairy cows

نویسندگان [English]

  • Arash Azarfar 1
  • Farshid Fattahnia 2
  • Reza Khodaakhshi 3
1 Associate Professor, Faculty of Agriculture, University of Lorestan, Khorram-Abad, Iran
2 Assistant Professor, Faculty of Agriculture, University of Ilam, Iran
3 Ph. D. Candidate, Faculty of Agriculture, University of Lorestan, Khorram-Abad, Iran
چکیده [English]

The objective of this study was to evaluate the effects of betaine supplementation (B-SUP) and vitamin B12 injection (VI-B12) during the transition period on serum metabolites in dairy cows. Thirty-two over conditioned multiparous Holstein cows assigned into 1 of 4 diets containing: CB (VI-B12), BC (50 g/day B-SUP), BB (VI-B12+ 50 g/day B-SUP) and control (CC) form 21 d before parturition (dbp) to 21 d postparturation (dpp). Blood sample was collected at -7, 2 and 42 dpp to determine serum metabolites of dairy cowand neonatal calves. The serum metabolites were not affected by experimental treatments (P>0.05), except for glucose which was greater in BC and CB as compared to CC and CB treatments (P<0.05). Among enzymatic indexes, a significant decrease was just showed in BB treatment as compared to CB treatment for GGT enzyme (p <0.05). Also, serum metabolites in neonatal calves were not influenced by experimental treatments (P>0.05). In conclusion, B-SUP and VI-B12 improved just glucose amount without any change in other blood parameters. Anyway, more researches can be give suitable results to us, particular if the protected form of the betaine is used.

کلیدواژه‌ها [English]

  • betaine
  • Dairy cow
  • serum metabolites
  • Transition period
  • vitamin B12
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