بررسی فراسنجه‏های تولیدی، بیوشیمیایی، ایمنی و پاداکسندگی در مرغ‏های تخم‏گذار های-لاین W-36 تغذیه‌شده با جیره‌های حاوی پادزیست محرک رشد و ترکیب افزودنی‏های خوراکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه ملایر

2 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه ملایر

3 استادیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه ملایر

چکیده

به‌منظور بررسی تأثیر برخی افزودنی‏های خوراکی در مقایسه با پادزیست (آنتی‏بیوتیک) محرک رشد فلاوومایسین، آزمایشی در قالب طرح کامل تصادفی با سه تیمار، پنج تکرار و بیست قطعه مرغ در هر تکرار با استفاده از 300 قطعه مرغ تخم‏گذار لگهورن سفید، سویه های-لاین W-36 انجام شد. تیمارهای آزمایشی شامل شاهد، پادزیست محرک رشد فلاوومایسین به میزان 05/0 درصد جیره و ترکیب افزودنی خوراکی شامل عصاره‏های گیاهی آویشن و پونۀ کوهی، اسید پروپیونیک و مکمل روی به میزان 1/0 درصد جیره بود که در سه دورۀ متوالی دو هفته‏ای درمجموع به مدت 42 روز برای تغذیه در اختیار مرغ‏های تخم‏گذار قرار داده شدند. نتایج این بررسی نشان دادند، استفاده از ترکیب افزودنی خوراکی سبب بهبود معنی‏داری در ضریب تبدیل خوراک، درصد تولید تخم‏مرغ و عیار پادتن (تیتر آنتی‌بادی) علیه آنفلوانزا و لیپوپروتئین با چگالی پایین در سرم خون نسبت به گروه شاهد شد (05/0≥P). مقادیر شاخص‏های پاداکسندگی (آنتی‏اکسیدانی) سرم خون شامل غلظت سلنیوم، ویتامین E، سوپراکسید دیسموتاز و گلوتاتیون پراکسیداز در مرغ­های تغذیه‌شده با جیرۀ حاوی ترکیب افزودنی خوراکی نسبت به دو گروه تیماری دیگر به‌صورت معنی‏داری افزایش یافت (05/0≥P). تیمارهای آزمایشی تأثیر معنی‏داری بر صفات کیفی تخم‏مرغ و فراسنجه‏های بیوشیمیایی خون به غیر از لیپوپروتئین با چگالی پایین نداشتند (05/0≤P). بنابر نتایج این آزمایش به نظر می­رسد که افزودنی خوراکی مورد بررسی می­تواند به‌عنوان جایگزین پادزیست محرک رشد در جیرۀ غذایی مرغ‏های تخم‏گذار استفاده شود.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of production, biochemical, immunity and antioxidant parameters in Hy-Line W- 36 laying hens fed diets containing growth promoter antibiotic and feed additive mixture

نویسندگان [English]

  • Milad Manafi 1
  • Fatemeh Mohebi 2
  • Mahdi Hedayati 3
  • Mojtaba Yari 3
1 Associate Professor, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran
2 Former M.Sc. Student, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran
3 Assistant Professors, Department of Animal Science, Faculty of Agricultural Sciences, Malayer University, Malayer, Iran
چکیده [English]

This research was conducted to evaluate the effect of some feed additives compare to Flavomycin growth promoter antibiotic. Experiment was conducted in completely randomized design manner consisting 3 treatments, 5 replicates and 20 hens per each replicate using 300 White Leghorn, Hy-Line W-36 strain. The experimental treatments included: control, Flavomycin growth promoter antibiotic at 0.05% dietary level and feed additive mixture consisting of thyme and oregano plant extracts and propionic acid and zinc supplement at 0.1% dietary level which fed to laying hens for 42 days (3 periods of two weeks each). The results of this study showed that the utilization of feed additive mixture significantly (P<0.05) improved feed conversion ratio, egg production percentage and blood serum antibody titers against avian Influanza and low-density lipoprotein compare to control group. The blood serum antioxidant indices including Selenium, vitamin E, Superoxide dismutase and Glutathione peroxidase were significantly (P<0.05) enhanced in laying hens fed diet containing feed additive mixture in comparison with two other treatment groups. The experimental treatments had no significant effect on egg qualitative traits and blood biochemical parameters with the exception of low-density lipoprotein. According to the results of this experiment, it seems that the studied feed additive mixture can be used as a replacement for growth promoter antibiotic in laying hen diets.

کلیدواژه‌ها [English]

  • Falvomycin
  • laying hens
  • oregano extract
  • Propionic acid
  • thyme extract
  • Zinc

مراجع

  1. Alçiçek, A., Bozkurt, M. & Çabuk, M. (2003). The effects of an essential oil combination derived from selected herbs growing wild in Turkey on broiler performance. South African Journal of Animal Science, 33, 89-94.
  2. Ali, M.N., Hassan, M.S. & Abd El-Ghany, F.A. (2007). Effect of strain, type of natural antioxidant and sulphate ion on productive, physiological and hatching performance of native laying hens. International Journal of Poultry Science, 6, 539-554.
  3. Azghadi, M., Pilevar, M., Arshami, J. & Mohamadkhani, A. (2010). Influence of various levels of thyme and cumin extracts on productive performance, egg quality and humoral immune responses of laying hens. The 4th Congress on Animal Science. 20-21 Sep, University of Tehran, Iran, 739-741. (in Farsi)
  4. Banerjee, P. & Keener, K.M. (2012). Maximizing carbon dioxide content of shell eggs by rapid cooling treatment and its effect on shell egg quality. Poultry Science, 91, 1444-1453.
  5. Bolukbasi, S. & Kuddusi, M. (2007). Effect of dietary thyme (Thymus vulgaris) on laying hens performance and Escherichia coli (E. coli) concentration in feces. International Journal of Natural and Engineering Sciences, 1, 55-58.
  6. Burrell, A.L., Dozier, W.A., Davis, A.J., Compton, M.M., Freeman, M.E., Vendrell, P.F. & Ward, T.L. (2004). Responses of broilers to dietary zinc concentrations and sources in relation to environmental implications. British Poultry Science, 45, 255-263.
  7. Çabuk, M., Bozkurt, M., Alicukek, A., Çatli, A.U. & Baser, K.H.C. (2006). Effect of a dietary essential oil mixture on performance of laying hens in the summer season. South African Journal of Animal Science, 36, 215-221.
  8. Chehrei, A., Nobakht, A. & Shahir, M.H. (2011). The effects of different levels of biohebal® feed supplement (contains thymus and garlic extracts) on performance, egg traits and blood biochemical and immunity parameters of laying hens. Veterinary Journal (Pajouhesh & Sazandegi), 90, 58-65. (in Farsi)
  9. Chen, J.Y., Latshaw, J.D., Lee, H.O. & Min, D.B. (1998). α-Tocopherol content and oxidative stability of egg yolk as related to dietary α-tocopherol. Journal of Food Science, 63, 919-922.
  10. Chvapil, M. (1973). New aspects in the biological role of zinc: a stabilizer of macromolecules and biological membranes. Life Sciences, 16, 1041-1049.
  11. Deans, S.G. & Waterman, P.G. (1993). Biological activity of volatile oils. Volatile oil crops: their biology, biochemistry and production. Longman Group, U.K. limited. 97-111.
  12. Edmonds, M.S. & Arentson, B.E. (2001). Effects of supplemental vitamins and trace minerals on performance and carcass quality in finishing pigs. Journal of Animal Science, 79, 141-147.
  13. Etches, R.J. (1999). Reproduction in Poultry. CAB International, Wallingford, UK.
  14. Fakler, T.M., Ward, T.L. & Kuhl, H.J. (2002). Zinc amino acid complexes improve layer production and egg quality. Poultry Science. 81 (Suppl. 1), 120 (Abstr.)
  15. Feyzi, A. & Bizhannezhad, P. (2010). Investigation effect of Thymus vulgaris essential oils on performance of broiler chickens. Veterinary Journal of Islamic AzadUniversity, Tabriz University. 12, 39-46. (in Farsi)
  16. Foster, S. & Duke, J. (1999). A field guide to medicinal plants and herbs of Eastern and central North America. Houghton Miffline Co., Boston, MA, USA. Pp. 411.
  17. Frankič, T., Voljč, M., Salobir, J. & Rezar, V. (2009). Use of herbs and spices and their extracts in animal nutrition. Acta Agriculture Slovenica, 94, 95-102.
  18. Ghasemi, R., Zarei, M. & Torki, M. (2010). Adding medicinal herbs including garlic (Allium Sativum) and thyme (Thymus Vulgaris) to diet of hens and evaluating productive performance and egg quality characteristics. American Journal of Animal Veterinary Science, 5, 151-154.
  19. Gheisari, A.A., Rahimi-Fathkoohi, A., Toghyani, M. & Gheisari, A.A. (2011). Influence of feeding diets supplemented with different levels and sources of zinc, copper and manganese on the mineral concentrations in tibia and performance of broiler chickens. Asian Journal of Animal and Veterinary Advances, 6, 166-174.
  20. Gulluce, M., Sahin, F., Sokmen, M., Ozer, H., Daferera, D., Sokme, A., Polissiou, M., Adiguzel, A. & Ozkan, H. (2007). Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chemistry, 103, 1449-1456.
  21. Guo, Y.M., Yang, R., Yuan, J., Ward, T.L. & Fakler, T.M. (2002). Effect of Availa Zn and ZnSO4 on laying hen performance and egg quality. Poultry Science, 81 (Suppl.), 40 (Abstr.)
  22. Hashemi, S.R. & Davoodi, H. (2012). New antibiotic replacements as growth and health promoters. Journal of Gorgan University of Medical Sciences, 13, 1-10. (in Farsi)
  23. Hedayati, M., Manafi, M., Yari, M. & Avara, A. (2014). The Influence of an acidifier feed additive on biochemical parameters and immune response of broilers. Annual Research & Review in Biology, 4, 1637-1645.
  24. Hempe, J.M., Laukxen, R.C. & Savage. J.E. (1988). Rapid determination of egg weight and specific gravity using a computerized data collection system. Poultry Science, 67, 902-907.
  25. Hernandez, F., Madrid, J., Gracia, V., Orengo, J. & Megias, M.D. (2004). Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poultry Science, 83, 169-174.
  26. Hoffman-Pennesi, D. & Wu, C. (2010). The effect of thymol and thyme oil feed supplementation on growth performance, serum antioxidant levels, and cecal Salmonella population in broilers. The Journal of Applied Poultry Research, 19, 432-443.
  27. Hy-line International Publication. (2006). Hy-Line variety W-36 commercial management guide. 2006-2008. West Des Moines, Iowa. USA.
  28. Isabel, B. & Santos, Y. (2009). Effects of dietary organic acids and essential oils on growth performance and carcass characteristics of broiler chickens. The Journal of Applied Poultry Research, 18, 472-476.
  29. Kalantar Neyestanaki, M., Saki, A., Zamani, P. & Ali Arabi, H. (2011). Effect of drinking thyme essence on performance, energy and protein efficiency and economical indices of broiler chickens. Animal Sciences Journal (Pajouhesh & Sazandegi), 92, 59-67. (in Farsi)
  30. Kirkpinar, F., Ünlü, H.B. & Özdemir, G. (2011). Effects of oregano and garlic essential oils on performance, carcass, organ and blood characteristics andıntestinal microflora of broilers. Livestock Science, 137, 219-225.
  31. Krosmayer, A. (2007). Experimental studies of the gastrointestinal effects of essential oils in comparison to avilamycin in weaned piglels. PhD. Dissertation. Universitat for Bodenkultur Wien, Austria.
  32. Lagouri, V., Blekas, G., Tsimidou, M., Kokkini, S. & Boskou, D. (1993). Composition and antioxidant activity of essential oils from Oregano plants grown wild in Greece. Zeitschrift für Lebensmittel-Untersuchung und Forschung, 197, 20-23.
  33. Lee, K.W., Kappert, H.J., Frehner, M., Losa, R. & Beynen, A.C. (2003). Effects of dietary essential oil components on growth performance, digestive enzymes and lipid metabolism in female broiler chickens. British Poultry Science, 44, 450-457.
  34. Leeson, S. & Summers, J.D. (2001). Scott's Nutrition of the Chicken. (4th ed). University Books, Guelph, Ontario, Canada.
  35. Leung, A.Y.A. & Foster, S. (1996). Encyclopedia of common natural ingredients used in food, drugs, and cosmetics. A Wiley Interscience Publication. John Wiley & Sons, Inc. Pp. 649.
  36. Mabe, I., Rapp, C., Bain, M.M. & Nyss, Y. (2003). Supplementation of a corn-soy bean meal diet with cupper, and zinc from organic or inorganic source improve eggshell quality in aged laying hens. Journal of Poultry Science, 82, 1903-1913.
  37. Manafi, M., Hedayati, M. & Yari, M. (2014). Effectiveness of Rosemary (Rosmarinus officinalis L.) essence on performance and immune parameters of broilers during Aflatoxicosis. Advances in Life Sciences, 4, 166-173.
  38. Marklund, S. & Marklund. G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Journal of Biochemistry, 47, 469-474.
  39. Mcclain, C.J., Gavaler, J.S. & Van-Thiel, D.H. (1984). Hypognadism in the zinc deficient rat: localization of the functional abnormalities. Journal of Laboratory and Clinical Medicine, 104, 1007-1015.
  40. Mohamadbagheri, N. & Najafi, R. (2014). Study the performance and blood lipid profile of broiler chickens supplemented with organic acids and phytase. Journal of Animal Science Reaserch, 24, 53-63. (in Farsi)
  41. Mohiti-Asli, M., Shivazad, M., Zaghari, M., Rezaian, M., Aminzadeh, S. &. Mateos, G.G. (2012). Effects of feeding regimen, fiber inclusion, and crude protein content of the diet on performance and egg quality and hatchability of eggs of broiler breeder hens. Poultry Science, 91, 3097-3106.
  42. Morales, J., Choi, J.S. & Kim, D.S. (2006). Production rate of propionic acid in fermentation of cheese whey withenzyme inhibitors. Environmental Progress, 25, 228-234.
  43. Moreno, L., Bell, R., Primo,-Yufera, E. & Esplugues, J. (2002). Pharmacological properties of the methanol extract from mentha suaveolens Ehrh. Phytotherapy Research, 16, 10-13.
  44. Nadali, M., Salari, S., Bojarpour, M., Tabataba, S. & Sari, M. (2013). Effects of different levels of zinc element on some of the productivity parameters of laying hens. Iranian Journal of Animal Science Reaserch, 5, 291-301. (in Farsi)
  45. Najafi, P. & Torki, M. (2010). Performance, blood metabolites and immuno-competaence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances, 9, 1164-1168.
  46. Nobakht, A. (2012). Effect of (Thymus vulgaris), (Urtica dioica L.) medicinal plants with (Medicago sativa) on performance, carcass parts, blood biochemical and immunity response of broiler chickens. Animal Science and Research Journal, 10, 59-72. (in Farsi)
  47. Nobakht, A. & Mehmannavaz, Y. (2010). Investigation the effects of using of Thymus vulgaris, Lamiaceae menthapiperita, Oreganum valgare medicinal plants on performance, egg quality, blood and immunity parameters of laying hens. Iranian Journal of Animal Science, 41, 129-136. (in Farsi)
  48. Nobakht, A., Safamehr, A., Nourani, J. & Moghdam, M. (2011). The effects of using different levels of pennyroyal (Mentha pulegium) medicinal plant on performance in broilers and laying hens. Animal Sciences Journal (Pajouhesh & Sazandegi), 92, 41-51. (in Farsi)
  49. Norouzi, E., Daneshyar, M. & Farhoomand, P. (2014). Effects of zinc acetate and magnesium sulfate on performance, carcass characteristics and blood indices of broilers under heat stress condition. Journal of Animal Science Reaserch, 24, 83-93. (in Farsi)
  50. North, M.O & Bell, D.D. (1990). Commercial chicken production manual. (4th ed). Van Nostrand Reinhold.
  51. Pagila, D.E. & Valentine, W.N. (1987). Methods of glutathione peroxidase activity assay. Journal of Laboratory and Clinical Medicine, 70, 158-159.
  52. Park, S.Y., Birkhold, S.G., Kubena, L.F., Nisbet, D.J. & Ricke, S.C. (2004).  Review on the role of dietary zinc in poultry nutrition, immunity, and reproduction. Biological Trace Element Research, 101, 147-163.
  53. Pazhohi Alamoti, M.R., Tajik, H., Akhondzade, A., Gandomi, H. & Ehsani, A. (2012). A study on chemical composition and antimicrobial activity of essential oil of Mentha longifolia L. and Cuminum cyminumL. in soup. Food Science & Technology Quarterly, 36, 33-45. (in Farsi)
  54. Pérez-Bonilla, A., Frikha, M., Mirzaie, S., García, J. & Mateos, G.G. (2011). Effects of the main cereal and type of fat of the diet on productive performance and egg quality of brown-egg laying hens from twenty-two to fifty-four weeks of age. Poultry Science, 90, 2801-2810.
  55. Pourreza, J. (2006). Scientific and Practical Principles of Poultry Farming. (8th ed), Jahade Daneshghahi Press. (Isfahan University of Technology).
  56. Raeisi, M., Safamehr, A., Khodaei Ashan, S. & Habibi, R. (2014). Thyme (Thymus vulgaris L.) and Oregano (Oreganum vulgare L.) essential oils for broilers: effect on performance, antioxidant indices and blood biochemical parameters. Animal Science Journal (Pajouhesh & Sazandegi), 105, 103-120. (in Farsi)
  57. Rahbarnia, B., Yaghoubfar, A., Korkoudi, K. & Kalantar Neyestanaki, M. (2013). Effect of thyme essence on growth traits, mortality, pH and changing of intestinal bacterial population in broiler chickens. Animal Sciences Journal (Pajouhesh & Sazandegi), 101, 37-45. (in Farsi)
  58. Rahmani, M., Nobakht, A. & Mehmannavaz, Y. (2012). Effect of different levels of pennyroyal, nettle and chicory medicinal plants with or without enzyme on performance, egg quality and blood biochemical parameters of laying hens. Journal of Livestock Research, 2, 19-28. (in Farsi)
  59. Rakhshan, M., Shivazad, M., Mousavi, S.N. & Zaghari, M. (2010). Effect of Biomus probiotic nutrition on small intestine morphology and cecum microbial population in broiler chickens. The 4th Congress of Animal Science. Sep. University of Tehran. Iran. 703-706. (in Farsi)
  60. Ritz, C.W., Hulet, R.M., Self, B.B. & Denbow, D.M. (1995). Growth and intestinal morphology of male turkeys as influenced by dietary supplementation of amylase and xylanase. Poultry Science, 74, 1329-1334.
  61. Roofchaee, A., Irani, M., Ebrahimzadeh, M.A. & Akbari, M.R. (2011). Effect of dietary oregano (Origanum vulgare L.) essential oil on growth performance, cecal microflora and serum antioxidant activity of broiler chickens. African Journal of Biotechnology, 10, 6177-6183.
  62. Rouse, J., Rolow, A. & Nelson, C.E. (1988). Effect of chemical treatment of poultry feed on survival of Salmonella. Poultry Science, 67, 1225-1228.
  63. Runho, R.C., Sakomura, N.K., Kuana, S., Banzatto, D., Junqueira, O.M. & Stringhini, J.H. (1997).  Use of an organic acid (fumaric acid) in broiler rations. Brazilian Journal of Animal Science, 26, 1183-1191.
  64. Safamer, A. & Attar-Hosseini, H. (2016). Effect of different leves of rice bran withand without multi-enzyme supplementation on performance, egg quality and serum biochemical parameters in commercial “Hy-line W-36” laying hens. Iranian Journal of Animal Science, 47, 19-29.
  65. Sahin, K., Sahin, N., Kucuk, O., Hayirli, A. & Prasad, A.S. (2009). Role of zinc in heat-stressed poultry. Poultry Science, 88, 2176-2183.
  66. Saki, A.A., Rahmati, M. & Najafi, S. (2009). Nutrition & Disease Management of Poultry. 1th edition, Bu-Ali Sina University Publication. 65-68. (in Farsi)
  67. Samman, S. & Cook, N.C. (1996). Flavonoids chemistry, metabolism, cardio protective effects, and dietary sources. Journal of Nutritional Biochemistry, 7, 66-76.
  68. Samsam-Shariat, S.H. (2004). Medicinal Plants Selective. (1th ed), Mani Publication. Pp. 93-309.
    (in Farsi)
  69. SAS Institute. (2005). SAS Users guide: Statistics. Version 9.12. SAS Institute Inc. Cary, NC.
  70. Seyedpiran, S.A., Nobakht, A. & Khodaee, S. (2011). Effects of using probiotic, organic acid and medicinal plants mixture on performance, egg quality and biochemical parameters and blood immunity of laying hens. Veterinary Journal of Islamic Azad University (Tabriz Unti), 17, 1111-1122. (in Farsi)
  71. Shyam Sunder, G., Panda, A.K., Gopinath, N.C.S., Rama Rao, S.V., Raju, M.V.L.N., Reddy, M.R. & Vijay Kumar, C.H. (2008). Effects of higher levels of zinc supplementation on performance, mineral availability and immune competence in broiler chickens. The Journal of Applied Poultry Research, 17, 79-86.
  72. Stevenson, L.M. & Jones, D.G. (1989). Relationships in between vitamin E status and erythrocyte stability in sheep. Journal of Comparative Pathology, 100, 359-368.
  73. Surai, P.F. (2002). Selenium in poultry nutrition: A newlook at an old element. 1. Antioxidant properties, deficiency, and toxicity. World's Poultry Science Journal, 58, 333-344.
  74. Tingetdy, R.P. (1989). Vitamin E immune responseand disease resistance. Annals of the New York Academy of Sciences, 570, 335-344.
  75. Toghyani, M., Tohidi, M., Ghaisari, A.A. & Tabaidian, S.A. (2010). Performance and dietary thyme as alternative immunity, biochemical and hematological parameters in broiler chicks fed for an antibiotic growth promoter. African Journal of Biotechnology, 9, 6819-6825.
  76. Uyanik, F., Eren, M. & Tuncoku, G. (2010). Effects of supplemental zinc on growth, serum glucose, cholesterol, enzymes and minerals in broiler. Pakistan Journal of Biological Science, 4, 745-747.
  77. Vakili, R. (2011). Effect of Fennel and Thymus vulgaris extracts with and without flaxseed on performance and eggs quality of laying hens. Iranian Journal of Animal Science, 3, 234-349. (in Farsi)
  78. Van As, P., Janssens, W., Onagbesan, O.M., Bruggeman, V., Buys, N., Sanders, J., Vandergeyten, S., Darras, V.M. & Decuypere, E. (2001). Quantification of growth hormone receptor extra- (GHRe) and intra-cellular domain (GHRi) gene expression in chicken liver by quantitative competitive RT-PCR. General & Comparative Endocrinology, 122, 213-224.
  79. Windisch, W., Schedle, K., Plitzner, C. & Kroismayr, A. (2007). Use of phytogenic products as feed additives for swine and poultry. Journal of Animal Science, 86, 140-148.
  80. Yalcin, S., Ebru, O., Zehra, R. & Suzan, Y. (2006). Effect of garlic powder on the performance, egg traits and blood parameters of laying hens. Journal of the Science of Food and Agriculture, 86, 1336-1339.
  81. Yan, F., Kersey, J.H. & Waldroup, P.W. (2001). Phosphorus requirements of broiler chicks three to six weeks of age as influenced by phytase supplementation. Poultry Science, 80, 455-459.
  82. Youdim, K.A. & Deans, S.G. (2000). Effect of thyme oil and thymol dietary supplementation on the antioxidant status and fatty acid composition of the ageing rat brain. British Journal of Nutrition, 83, 87-93.
  83. Zamani, A., Rahmani, H.R. & Pourreza, J. (2009). Effect of different levels of manganese and zinc on shells with translucent areas, Haugh unit, mortality, percentage of ash in eggshell and composition of eggshell in laying hens. Iranian Journal of Animal Science Reaserch, 2, 37-45. (in Farsi)
  84. Zamiri, M.J. (2009). Physiology of Reproduction. (2th ed). Haghshenas Publication. Pp. 371-373. (in Farsi)

 

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  • تاریخ دریافت: 20 دی 1394
  • تاریخ بازنگری: 06 خرداد 1397
  • تاریخ پذیرش: 08 خرداد 1397

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