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تأثیر بنتونیت نانوساختار پیلارد بر عملکرد و فراسنجه‌های بیوشیمیایی خون جوجه‌های گوشتی چالش‌یافته با آفلاتوکسین

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، دانشگاه بیرجند، بیرجند، ایران

2 استادیار، گروه علوم دامی، دانشگاه بیرجند، بیرجند، ایران

3 استاد، گروه علوم دامی، دانشگاه بیرجند، بیرجند، ایران

4 استادیار گروه شیمی، دانشگاه بیرجند، بیرجند، ایران

چکیده

هدف از این آزمایش، بررسی تأثیر بنتونیت سدیم پیلارد (PB) ‌بر عملکرد، وزن نسبی اجزاء لاشه و فراسنجه‌های بیوشیمیایی خون جوجه‌های گوشتی چالش‌یافته با آفلاتوکسینبود. بدین منظور از 128 قطعه جوجه‌خروس یک‌روزه راس 308 در یک طرح کامل تصادفی با چهار تیمار و چهار تکرار و هشت پرنده در هر تکرار به مدت 35 روز استفاده شد. تیمارهای آزمایشی شامل: شاهد منفی (C)، شاهد مثبت (جیرۀ پایه + آفلاتوکسینAFB1) و دو سطح 25/0 و 75/0 درصد بنتونیت نانو ساختار پیلارد (PB) که به جیرۀ پایۀ آلوده به آفلاتوکسینAFB1اضافه شد، میزان آفلاتوکسین در جیره‌های آلوده در دورۀ آغازین و رشد برابر ppb500 و در پایانی ppb1000 بود. ستون حایل بودن (پیلارینگ) در بنتونیت سدیم باعث افزایش میزان اکسید آهن از 99/1 به 42/8 درصد و در بنتونیت سدیم از 43/2 به 43/0 درصد در بنتونیت پیلارد کاهش یافت. آفلاتوکسین باعث افزایش FCRو فعالیت آنزیم‌های ALT و ALP در مقایسه با شاهد منفی شد. افزودن 25/0 درصد PB به جیرۀ آلوده باعث افزایش مصرف خوراک، وزن بدن و کاهش ALT شد. افزودن 75/0 درصد PB باعث کاهش ALT و ALP در مقایسه با تیمار آلوده شد. بنابراین بنتونیت سدیم پیلارد باعث بهبود عملکرد و کاهش فعالیت آنزیم‌های کبدی در جوجه‌های تغذیه‌شده با آفلاتوکسین می‌شود. نتایج به‌دست‌آمده در این بررسی، آفلاتوکسین در سطح 1000 نانوگرم در کیلوگرم اثری بر وزن بدن و مصرف خوراک نداشت و سطح 25/0 درصد بنتونیت پیلارد مناسب­ترین پاسخ عملکردی را در جوجه­های تغذیه‌شده با آفلاتوکسین داشت.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Pillaring bentonite nanostructure on performance and blood parameters of broiler chicken challenged with Aflatoxin B1

نویسندگان [English]

  • Hassan Kihani-yazdi 1
  • Seyyed Javad Hosseini-Vashan 2
  • Nazar Afzali 3
  • Mohsen Mojtahedi 2
  • Ali Allahressani 4
1 Former M. Sc. Student, Department of Animal Science , University of Birjand, Birjand, I.R. Iran
2 Assistant Professor, Department of Animal Science , University of Birjand, Birjand, I.R. Iran
3 Professor, Department of Animal Science, University of Birjand, Birjand, I.R. Iran
4 Assistant Professor, Department of Chemistry, University of Birjand, Birjand, I.R. Iran
چکیده [English]

The aim of this study was to evaluate the effects of Pillaring bentonite nanostructure on growth performance, carcass components and blood parameters of broilers challenged by aflatoxin (AF). A total of 128 day-old Ross 308 broiler chicks in a completely randomized design were randomly allotted to 4 treatments, 4 replicates and 8 birds in each. The birds were reared for 35 days. Experimental groups consisted of: negative control (C), positive control (basal diet with aflatoxin) (AF) and two level 0.25 and 0.75 % of Pillared bentonite added to basal diet with aflatoxin B1 (positive control). The Pillaring process increased the concentration FeO (1.99 to 8.42%) and decreased concentration Na2O (2.43 to 0.43 %) in Pillared bentonite as compared to sodium bentonite. The aflatoxin increased feed conversion ratio and enzyme activity ALP and ALT as compared to control diet. Supplementation of 0.25% Pillared bentonite to the infected diet of broilers increased the feed intake and body weight and decreased the concentration of ALT. Addition  0.75% Pillared bentonite increased the concentration of ALT and ALP as compare to  positive control. Therefore, the supplementation of Pillared bentonite nanostructure to broiler diets infected with aflatoxin improved the performance and reduced the liver enzyme activity. The results of present research showed that Aflatoxin may had no effect on body weight and feed intake of broilers and the levels of 0.25 Pillared bentonite nanostructure has the best efficient on performance of broiler fed diets contained aflatoxin.

کلیدواژه‌ها [English]

  • Abdominal Fat
  • cholesterol
  • feed conversion ratio
  • liver enzyme
  • Lactate Dehydrogenase
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