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بررسی بیان ژن تریپسین در بافت پانکراس جوجه‌های گوشتی تغذیه‌شده با آرد بلوط

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، دانشکدۀ کشاورزی، دانشگاه یاسوج

2 دانشیار، دانشکدۀ کشاورزی، دانشگاه یاسوج

چکیده

هدف از این بررسی ارزیابی بیان ژن تریپسین در بافت پانکراس جوجه­های گوشتی تغذیه‌شده با جیره‌های بلوط تانن­دار بود. در این آزمایش، از سه جیره (شاهد یا کنترل، جیرۀ حاوی 15 درصد و جیرۀ حاوی 20 درصد بلوط) برای تغذیۀ جوجه‌های گوشتی در طول دورۀ 42 روزه استفاده شد. در روزهای 21 و 42 روزگی، از 36 جوجۀ گوشتی (18 جوجه برای هر زمان، شش جوجه برای هر تیمار) RNA کل از بافت پانکراس استخراج شد. برای بررسی بیان ژن تریپسین، از ژن بتا اکتین به‌عنوان ژن مرجع استفاده شد. برای تجزیۀ داده‌های بیان ژن از نرم‌افزار REST,  2009, V2.0.13 و SAS استفاده شد. نتایج نشان داد، وزن نسبی پانکراس در سن 21 روزگی در بین تیمارهای آزمایشی تفاوت نداشت، اما در سن 42 روزگی، وزن پانکراس در تیمار 20 درصد نسبت به تیمارهای شاهد و 15 درصد افزایش معنی‌داری داشت. بیان ژن تریپسین در سن 21 روزگی در تیمار 15 درصد نسبت به تیمار شاهد تغییر معنی‏داری نداشت اما در همین سن بیان ژن تریپسین در تیمار 20 درصد نسبت به تیمار شاهد به‌طور معنی‌داری افزایش یافت. در سن 42 روزگی در تیمار 15 و 20 درصد بیان ژن تریپسین نسبت به تیمار شاهد تفاوت معنی‌داری نداشت. بر پایۀ نتایج این بررسی، در سن 21 روزگی سطح 20 درصد بلوط در جیره منجر به افزایش بیان ژن تریپسین شد، درحالی‌که در سن 42 روزگی  استفاده از 15 و 20 درصد بلوط تغییر معنی‌داری در بیان ژن تریپسین ایجاد نکرد. همچنین، همبستگی معنی‌داری بین بیان ژن تریپسین در پانکراس و وزن نسبی پانکراس در روز 42 روزگی مشاهده شد (01/0P<).

کلیدواژه‌ها


عنوان مقاله [English]

Expression analysis of trypsin gene in pancreatic tissue of broiler chicken fed diets containing oak acorn

نویسندگان [English]

  • Fatemeh Ghorbani 1
  • Mustafa Muhaghegh-Dolatabady 2
1 M.Sc. Student, Faculty of Agriculture, Yasouj University, Yasouj, Iran
2 Associate Professor, Faculty of Agriculture, Yasouj University, Yasouj, Iran
چکیده [English]

Recently, the significance of oak as an alternative feed for corn replacement in chicken diets is well documented. However, this feed contains antinutrien factor called tannin that has limited use of this feed in the poultry diets. One of these restrictions is combination of tannins with diets proteins that lead to disturbance in function of digestive enzymes. Therefore, the aim of this study was to investigate the expression of trypsin gene in the pancreas tissue of broiler chicken fed tannin containing oak diets. In this experiment, three diets (control, 15% and 20% oak) were fed to broilers from 1 to 42 days of age. On days 21 and 42, total RNA extracted from pancreatic tissue of 36 broiler chicken (18 for each time, 6 for each treatment). To study of gene expression, the trypsin expression was compared with β-actin gene as a reference gene. For gene expression analysis, the REST, V2.0.13 2009 software was used. No significant association was found between pancreases relative weight and different treatments on day 21, but the relation between the relative weight of pancreases and diets on day 42 was significant for 20 percent of oak acorn. The mRNA expression level of trypsin gene was significantly higher in the 20 percent of oak acorn on day 21, but their differences were not significant in compared to control diet on day 42. In addition, significant correlation was found between gene expression of trypsin and relative weight of pancreases on day 42.

کلیدواژه‌ها [English]

  • broiler
  • gene expression
  • Oak
  • trypsin
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