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تأثیر لتروزل خوراکی بر غلظت گونادوتروپین های پلاسما و برخی فراسنجه های بافت شناسی بیضه در خروس مادر گوشتی در 40 هفتگی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

4 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

در این پژوهش تأثیر لتروزل خوراکی بر غلظت LH و FSH پلاسما و برخی فراسنجه­های بافت­شناسی بیضۀ خرو­س­های گلۀ مادر سویۀ راس 308 بررسی شد. بیست قطعه خروس با سن 40 هفته به‌صورت تصادفی به چهار گروه یکسان تقسیم و در جایگاه (پن)­های انفرادی توزیع شدند. تیمارهای آزمایشی شامل دزهای مختلف لتروزل (0 (L0)، 5/0 (L0.5)، 1 (L1) و 5/1 (L1.5) میلی­گرم به ازای هر پرنده در روز) بوده که به مدت 12 هفته به پرندگان خورانده شد. خون­گیری از پرندگان در هفته­های اول، سوم، پنجم، هفتم و نهم و نمونه­­برداری از بافت بیضه در انتهای آزمایش (هفتۀ دوازدهم) انجام شد. بنا بر نتایج، میزان FSH و LH پلاسمای خون، قطر و ضخامت بافت پوششی (اپیتلیوم) لولۀ اسپرم­ساز و شمار یاخته­های لایدیگ در گروه­های تغذیه‌شده با لتروزل نسبت به شاهد به‌طور معنی­داری افزایش یافت و در بین گروه­های آزمایشی نیز میزان FSH و LH به ترتیب در گروه­های L1 (11/0±39/10 و 08/0±93/2) و L1.5 (2/0±5/9 و 08/0±17/3) بالاترین بود. شمار یاخته­های اسپرماتوگونی در دو گروه L0.5 و L1 نسبت به شاهد به‌صورت معنی­داری بهبود یافت، اما شمار رگ­های خونی تغییر معنی­داری نداشت. به‌طور خلاصه، تجویز خوراکی لتروزل (1 و 5/1 میلی­گرم) توانست غلظت گونادوتروپین­ها و برخی فراسنجه­های بافت‌شناسی بیضۀ خروس­های مادر گوشتی را بهبود دهد، اما برای تأیید این نتایج و تعمیم آن‌ها به باروری نهایی خروس­ها به بررسی‌های بیشتری نیاز است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of dietary Letrozole on plasma gonadotropin concentrations and some testicular histology parameters in 40 weeks old broiler breeder rooster

نویسندگان [English]

  • Emad Abdulgabbar Ali 1
  • Mahdi Zhandi 2
  • Armin Towhidi 3
  • Mojtaba Zaghari 3
  • Mahdi Ansari 4
1 Ph. D. Candidate, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Ph. D. Candidate, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The current study was carried out to evaluate the effect of Letrozole feeding on plasma LH and FSH concentrations and testicular histology in Ross 308 broiler breeder roosters. Twenty 40-week old roosters were randomly divided into four groups, individually housed and fed different levels of capsulated Letrozole including 0 (L0), 0.5 (L0.5), 1 (L1) or 1.5 (L1.5) mg/bird/day for 12 consecutive weeks. Blood sampling was done on 1th, 3th, 5th, 7th and 9th weeks of trail. Testicular histology was assessed on samples from last week (12th) of experiment. According to the results, plasma LH and FSH levels, seminiferous tubule diameter and epithelium thickness as well as Leydig cell numbers were significantly increased in Letrozole treated groups compered to control group. The highest concentration of FSH and LH were recorded for L1 (10.39 ±0.11 and 2.93±0.08) and L1.5 (9.5±0.2 and 3.17±0.08) groups, respectively. Both L0.5 and L1 groups had significantly higher spermatogonial numbers than control group. However, blood vessel numbers were not significantly affected. In conclusion, orally feeding Letrozole (1 and 1.5 mg) could improve gonadotropin concentration and testicular histology parameters of male broiler breeders at 40 weeks of age. Further studied are needed to confirm these results and extend them to final fertility.

کلیدواژه‌ها [English]

  • Gonadotropin
  • letrozole
  • Rooster
  • spermatogonium
  • testis histology
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