تأثیر لتروزل بر کیفیت و باروری اسپرم‌ منجمد- ذوب‌شده در خروس‌های مادر گوشتی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

در این پژوهش تأثیر تجویز خوراکی لتروزل بر غلظت تستوسترون پلاسما و کیفیت و باروری اسپرم خروس­های مادر گوشتی پس از انجماد-یخ­گشایی بررسی شد. بیست قطعه خروس سویۀ راس 308 با سن 50 هفته به‌صورت تصادفی به چهار گروه پنج­تایی در پن­های انفرادی تقسیم و سطوح 0 (کنترل)، 5/0(L0.5)، 1 (L1)، 5/1 (L1.5) میلی­گرم لتروزل به ازای هر پرنده در روز دریافت کردند. اسپرم­گیری و گرد­آوری نمونۀ خون برای اندازه­گیری تستوسترون به‌صورت هفتگی و به ترتیب به مدت هفت و شش هفته انجام گرفت. در نمونه­های مربوط به چهار هفته اول، فراسنجه­های جنبایی کل و پیش­رونده، زنده­مانی و فعالیت غشای پلاسمایی پیش از انجماد و فراسنجه­های یادشده و درصد اسپرم­های نابهنجار پس از انجماد-یخ­گشایی بررسی شد و نمونه­های سه هفتۀ بعدی پس از انجماد-یخ­گشایی برای ارزیابی نفوذ اسپرم و نرخ باروری استفاده شد. بنا بر نتایج، جنبایی کل و پیش­رونده و زندمانی پیش و پس از انجماد-یخ­گشایی در گروه­های تغذیه‌شده با لتروزل نسبت به گروه شاهد به‌طور معنی­داری بالاتر بود. همچنین فعالیت غشای پلاسمایی پیش و پس از انجماد-یخ­گشایی و میزان نفوذ و باروری در گروه­های L0.5 و L1  نسبت به گروه شاهد به‌طور معنی­داری بهبود یافت اما تستوسترون در گروه L1.5 نسبت به گروه شاهد افزایش معنی­داری داشت. با وجود مشاهدۀ تأثیر منفی در سطح 5/1 میلی‌گرم لتروزل، به نظر می­رسد سطوح 5/0میلی­گرم لتروزل برای هر پرنده در روز برای بهبود قابلیت انجماد اسپرم خروس قابل استفاده بوده، اما برای تأیید نتایج این پژوهش، نیاز به بررسی‌های بیشتری است.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of Letrozole on frozen-thawed sperm quality and fertility of broiler breeder roosters

نویسندگان [English]

  • Mahbub Mohammadi 1
  • Ahmad Zare-Shahaneh 2
  • Saeid Zeinoaldini 3
  • Mahdi Ansari 4
1 M.Sc. student, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Ph.D. Condidate, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This study was carried out to evaluate the effect of the oral administration of Letrozole on blood testosterone level and frozen-thawed sperm quality and fertility of broiler breeder roosters. Twenty 50-week-old Ross 308 roosters were randomly divided into four groups (five roosters per each), individually housed in pens and received 0 (Control), 0.5 (L0.5), 1 (L1) or 1.5 (L1.5) mg Letrozole/bird/day. Semen and blood collections were done weekly for seven and six weeks, respectively. Sperm quality parameters including total and forward motility, viability and plasma membrane functionality were assessed prior freezing and these parameters along with abnormality were evaluated using post-thawed semen samples. The last three weeks were frozen and artificially inseminated to evaluate sperm penetration and fertility rate. According to the results, total and forward motility and viability of fresh and frozen-thawed sperm were significantly higher in Letrozole treated groups compared to control group. Plasma membrane functionality of fresh and frozen-thawed sperm as well as penetration and fertility rates were significantly improved in both L0.5 and L1 groups compared to control group. However, blood testosterone level was significantly higher in L1.5 than control group. In spite of observing negative effects in 1.5 level, both 0.5 mg of Letrozole/bird/day could improve sperm freezability of roosters, however, further research are needed to ascertain this results.

کلیدواژه‌ها [English]

  • Cryopreservation
  • fertility
  • letrozole
  • Sperm
  • Testosterone

مراجع

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سابقه مقاله

  • تاریخ دریافت: 26 دی 1395
  • تاریخ بازنگری: 30 فروردین 1396
  • تاریخ پذیرش: 30 فروردین 1396

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