8954856055505db

تأثیر لتروزل بر کیفیت و باروری اسپرم‌ منجمد- ذوب‌شده در خروس‌های مادر گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 دانشجوی دکتری، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

در این پژوهش تأثیر تجویز خوراکی لتروزل بر غلظت تستوسترون پلاسما و کیفیت و باروری اسپرم خروس­های مادر گوشتی پس از انجماد-یخ­گشایی بررسی شد. بیست قطعه خروس سویۀ راس 308 با سن 50 هفته به‌صورت تصادفی به چهار گروه پنج­تایی در پن­های انفرادی تقسیم و سطوح 0 (کنترل)، 5/0(L0.5)، 1 (L1)، 5/1 (L1.5) میلی­گرم لتروزل به ازای هر پرنده در روز دریافت کردند. اسپرم­گیری و گرد­آوری نمونۀ خون برای اندازه­گیری تستوسترون به‌صورت هفتگی و به ترتیب به مدت هفت و شش هفته انجام گرفت. در نمونه­های مربوط به چهار هفته اول، فراسنجه­های جنبایی کل و پیش­رونده، زنده­مانی و فعالیت غشای پلاسمایی پیش از انجماد و فراسنجه­های یادشده و درصد اسپرم­های نابهنجار پس از انجماد-یخ­گشایی بررسی شد و نمونه­های سه هفتۀ بعدی پس از انجماد-یخ­گشایی برای ارزیابی نفوذ اسپرم و نرخ باروری استفاده شد. بنا بر نتایج، جنبایی کل و پیش­رونده و زندمانی پیش و پس از انجماد-یخ­گشایی در گروه­های تغذیه‌شده با لتروزل نسبت به گروه شاهد به‌طور معنی­داری بالاتر بود. همچنین فعالیت غشای پلاسمایی پیش و پس از انجماد-یخ­گشایی و میزان نفوذ و باروری در گروه­های L0.5 و L1  نسبت به گروه شاهد به‌طور معنی­داری بهبود یافت اما تستوسترون در گروه L1.5 نسبت به گروه شاهد افزایش معنی­داری داشت. با وجود مشاهدۀ تأثیر منفی در سطح 5/1 میلی‌گرم لتروزل، به نظر می­رسد سطوح 5/0میلی­گرم لتروزل برای هر پرنده در روز برای بهبود قابلیت انجماد اسپرم خروس قابل استفاده بوده، اما برای تأیید نتایج این پژوهش، نیاز به بررسی‌های بیشتری است.

کلیدواژه‌ها


عنوان مقاله [English]

The effect of Letrozole on frozen-thawed sperm quality and fertility of broiler breeder roosters

نویسندگان [English]

  • Mahbub Mohammadi 1
  • Ahmad Zare-Shahaneh 2
  • Saeid Zeinoaldini 3
  • Mahdi Ansari 4
1 M.Sc. student, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
4 Ph.D. Condidate, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This study was carried out to evaluate the effect of the oral administration of Letrozole on blood testosterone level and frozen-thawed sperm quality and fertility of broiler breeder roosters. Twenty 50-week-old Ross 308 roosters were randomly divided into four groups (five roosters per each), individually housed in pens and received 0 (Control), 0.5 (L0.5), 1 (L1) or 1.5 (L1.5) mg Letrozole/bird/day. Semen and blood collections were done weekly for seven and six weeks, respectively. Sperm quality parameters including total and forward motility, viability and plasma membrane functionality were assessed prior freezing and these parameters along with abnormality were evaluated using post-thawed semen samples. The last three weeks were frozen and artificially inseminated to evaluate sperm penetration and fertility rate. According to the results, total and forward motility and viability of fresh and frozen-thawed sperm were significantly higher in Letrozole treated groups compared to control group. Plasma membrane functionality of fresh and frozen-thawed sperm as well as penetration and fertility rates were significantly improved in both L0.5 and L1 groups compared to control group. However, blood testosterone level was significantly higher in L1.5 than control group. In spite of observing negative effects in 1.5 level, both 0.5 mg of Letrozole/bird/day could improve sperm freezability of roosters, however, further research are needed to ascertain this results.

کلیدواژه‌ها [English]

  • Cryopreservation
  • fertility
  • letrozole
  • Sperm
  • Testosterone
  1. Abouelezz, F. M., Castano, C., Toledano-Diaz, A., Esteso, M. C., Lopez-Sebastian, A., Campo, J. L. & Santiago-Moreno, J. (2015). Effect of the interaction between cryoprotectant concentration and cryopreservation method on frozen/thawed chicken sperm variables. Reproduction in Domestic Animals, 50(1), 135-141.
  2. Akhlaghi, A., Ahangari, Y. J., Navidshad, B., Pirsaraei, Z. A., Zhandi, M., Deldar, H., Peebles, E. D. (2014). Improvements in semen quality, sperm fatty acids, and reproductive performance in aged Cobb 500 breeder roosters fed diets containing dried ginger rhizomes (Zingiber officinale). Poultry Science, 93(5), 1236-1244.
  3. Akhlaghi, A., Jafari Ahangari, Y., Zhandi, M. & Peebles, E. D. (2014). Reproductive performance, semen quality, and fatty acid profile of spermatozoa in senescent broiler breeder roosters as enhanced by the long-term feeding of dried apple pomace. Animal Reproduction Science, 147(1-2), 64-73.
  4. Ansari, M., Zhandi, M., Kohram, H., Zaghari, M., Sadeghi, M. & Sharafi, M. (2017). Improvement of post-thawed sperm quality and fertility of Arian rooster by oral administration of D-aspartic acid. Theriogenology.
  5. Blesbois, E., Grasseau, I. & Seigneurin, F. (2005). Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction, 129(3), 371-378.
  6. Blesbois, E., Grasseau, I., Seigneurin, F., Mignon-Grasteau, S., Saint Jalme, M. & Mialon-Richard, M. M. (2008). Predictors of success of semen cryopreservation in chickens. Theriogenology, 69(2), 252-261.
  7. Bramwell, R. K., McDaniel, C. D., Wilson, J. L. & Howarth, B. (1996). Age effect of male and female broiler breeders on sperm penetration of the perivitelline layer overlying the germinal disc. Poultry Science, 75(6), 755-762.
  8. Burrows, W. & Quinn, J. (1936). The Collection of Spermatozoa from the Domestic Fowl and Turkey. Poultry Science, 16(1), 19-24.
  9. Buss, E. G. (1993). Cryopreservation of rooster sperm. Poultry Science, 72(5), 944-954.
  10. Cardone, A., Comitato, R., Bellini, L. & Angelini, F. (2002). Effects of the aromatase inhibitor fadrozole on plasma sex steroid secretion, spermatogenesis and epididymis morphology in the lizard, Podarcis sicula. Molecular Reproduction and Development, 63(1), 63-70.
  11. Carreau, S., Bourguiba, S., Lambard, S., Galeraud-Denis, I., Genissel, C., Bilinska, B., Levallet, J. (2001). Aromatase expression in male germ cells. Journal of Steroid Biochemistry & Molecular Biology, 79(1-5), 203-208.
  12. Carreau, S., de Vienne, C. & Galeraud-Denis, I. (2008). Aromatase and estrogens in man reproduction: a review and latest advances. Advances in Medical Sciences, 53(2), 139-144.
  13. Carreau, S., Delalande, C. & Galeraud-Denis, I. (2009). Mammalian sperm quality and aromatase expression. Microscopy Research and Technique, 72(8), 552-557.
  14. Carreau, S., Silandre, D., Bois, C., Bouraima, H., Galeraud-Denis, I. & Delalande, C. (2007). Estrogens: a new player in spermatogenesis. Folia Histochemica et Cytobiologica, 11(3), 174-193.
  15. Cavallini, G., Beretta, G. & Biagiotti, G. (2011). Preliminary study of letrozole use for improving spermatogenesis in non-obstructive azoospermia patients with normal serum FSH. Asian Journal of Andrology, 13(6), 895-897.
  16. Cerolini, S., Zaniboni, L., Maldjian, A. & Gliozzi, T. (2006). Effect of docosahexaenoic acid and alpha-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66(4), 877-886.
  17. Dimitriadis, F., Tsiampali, C., Chaliasos, N., Tsounapi, P., Takenaka, A. & Sofikitis, N. (2015). The Sertoli cell as the orchestra conductor of spermatogenesis: spermatogenic cells dance to the tune of testosterone. Hormones (Athens), 14(4), 479-503.
  18. Dong, H. J., Wu, D., Xu, S. Y., Li, Q., Fang, Z. F., Che, L. Q., Lin, Y. (2016). Effect of dietary supplementation with amino acids on boar sperm quality and fertility. Animal Reproduction Science, 172, 182-189.
  19. Esteves, S. C., Spaine, D. M., Cedenho, A. P. & Srougi, M. (2003). Effects of the technique of cryopreservation and dilution/centrifugation after thawing on the motility and vitality of spermatozoa of oligoasthenozoospermic men. International Brazilian Journal of Urology, 29(2), 133-140.
  20. Gregoriou, O., Bakas, P., Grigoriadis, C., Creatsa, M., Hassiakos, D. & Creatsas, G. (2012). Changes in hormonal profile and seminal parameters with use of aromatase inhibitors in management of infertile men with low testosterone to estradiol ratios. Fertility and Sterility, 98(1), 48-51.
  21. Holt, W. V. (2000). Basic aspects of frozen storage of semen. Animal Reproduction Science, 62(1-3), 3-22.
  22. Long, J. A. (2006). Avian semen cryopreservation: what are the biological challenges? Poultry Science, 85(2), 232-236.
  23. Lukaszewicz, E., Jerysz, A., Partyka, A & .Siudzinska, A. (2008). Efficacy of evaluation of rooster sperm morphology using different staining methods. Research in Veterinary Science, 85(3), 583-588.
  24. Miura, T., Yamauchi, K., Takahashi, H. & Nagahama, Y. (1992). The role of hormones in the acquisition of sperm motility in salmonid fish. Journal of experimental zoology, 261(3), 359-363.
  25. Moghbeli, M., Kohram, H., Zare-Shahaneh, A., Zhandi, M., Sharafi, M., Nabi, M. M., Sharideh, H. (2016). Are the optimum levels of the catalase and vitamin E in rooster semen extender after freezing-thawing influenced by sperm concentration? Cryobiology, 72(3), 264-268.
  26. Nabi, M. M., Kohram, H., Zhandi, M., Mehrabani-Yeganeh, H., Sharideh, H., Zare-Shahaneh, A. & Esmaili, V. (2016). Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen. Cryobiology, 72(1), 47-52.
  27. O’donnell, L., Robertson, K. M., Jones, M. E. & Simpson, E. R. (2001). Estrogen and spermatogenesis. Endocrine Reviews, 22(3), 22.
  28. Patry, G., Jarvi, K., Grober, E. D. & Lo, K. C. (2009). Use of the aromatase inhibitor letrozole to treat male infertility. Fertility and Sterility, 92(2), 829.e1-829.e2.
  29. Raman, J. D. & Schlegel, P. N. (2002). Aromatase inhibitors for male infertility. The Journal of Urology, 167(2 Pt 1), 624-629.
  30. Raven, G., de Jong, F. H., Kaufman, J. M. & de Ronde, W. (2006). In men, peripheral estradiol levels directly reflect the action of estrogens at the hypothalamo-pituitary level to inhibit gonadotropin secretion. The Journal of Clinical Endocrinology & Metabolism, 91(9), 3324-3328.
  31. Romero-Sanchez, H., Plumstead, P. W. & Brake, J. (2007). Feeding broiler breeder males. 1. Effect of feeding program and dietary crude protein during rearing on body weight and fertility of broiler breeder males. Poultry Science, 86(1), 168-174.
  32. Saylam, B., Efesoy, O. & Cayan, S. (2011). The effect of aromatase inhibitor letrozole on body mass index, serum hormones, and sperm parameters in infertile men. Fertility and Sterility, 95(2), 809-811.
  33. Séralini, G.-E. & Moslemi, S. (2001). Aromatase inhibitors: past, present and future. Molecular and Cellular Endocrinology, 178(1–2), 117-131.
  34. Shaha, C. (2008). Estrogens and spermatogenesis. Advances in experimental medicine and biology, 636, 42-64.
  35. Shahverdi, A., Sharafi, M., Gourabi, H., Yekta, A. A., Esmaeili, V., Sharbatoghli, M., Mostafayi, F. (2015). Fertility and flow cytometric evaluations of frozen-thawed rooster semen in cryopreservation medium containing low-density lipoprotein. Theriogenology, 83(1), 78-85.
  36. Sharideh, H., Esmaeile Neia, L., Zaghari, M., Zhandi, M., Akhlaghi, A. & Lotfi, L. (2016). Effect of feeding guanidinoacetic acid and L-arginine on the fertility rate and sperm penetration in the perivitelline layer of aged broiler breeder hens. Journal of Animal Physiology and Animal Nutrition, 100(2), 316-322.
  37. Shetty, G., Krishnamurthy, H., Krishnamurthy, H. N., Bhatnagar, S. & Moudgal, R. N. (1997). Effect of estrogen deprivation on the reproductive physiology of male and female primates. The Journal of Steroid Biochemistry and Molecular Biology, 61(3-6), 157-166.
  38. Smith, L. B. & Walker, W. H. (2014). The regulation of spermatogenesis by androgens. Seminars in Cell & Developmental Biology, 30, 2-13.
  39. T’Sjoen, G. G., Giagulli, V. A., Delva, H., Crabbe, P., De Bacquer, D. & Kaufman, J.-M. (2005). Comparative assessment in young and elderly men of the gonadotropin response to aromatase inhibition. The Journal of Clinical Endocrinology & Metabolism, 90(10), 5717-5722.
  40. Thurlimann, B., Keshaviah, A., Coates, A. S., Mouridsen, H., Mauriac, L., Forbes, J. F., Goldhirsch, A. (2005). A comparison of letrozole and tamoxifen in postmenopausal women with early breast cancer. The New England Journal of Medicine, 353(26), 2747-2757.
  41. Weil, S., Rozenboim, I., Degen, A. A., Dawson, A., Friedländer, M. & Rosenstrauch, A. (1999). Fertility Decline in Aging Roosters Is Related to Increased Testicular and Plasma Levels of Estradiol. General and Comparative Endocrinology, 115(1), 23-28.