8954856055505db

ذخیره‌سازی منی خروس به‌صورت مایع با استفاده از عصارۀ الکلی رزماری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، دانشکدة علوم کشاورزی، دانشگاه گیلان

2 دانشیار، گروه علوم دامی، دانشکدة علوم کشاورزی، دانشگاه گیلان

چکیده

این آزمایش برای بررسی عصارۀ الکلی برگ رزماری بر ذخیره­سازی اسپرم خروس در °C 4 آزمایشی با استفاده از هشت قطعه خروس بالغ انجام شد. گرد­آوری منی سه روز یک‌بار در پنج نوبت انجام شد. انزال­ها در هر نوبت پس از تجمیع به شش قسمت تقسیم و مقدار 0 (R0)، 10 (R10)،  20(R20)،30 (R40)، 40 و 50 (R50)  میکروگرم عصارۀ الکلی رزماری در میلی­لیتر به هر قسمت اضافه شد. سپس نمونه­ها تا °C 4 سرد شد و به­مدت 72 ساعت در این دما نگهداری شد. در زمان­های 0 (T0)، 24 (T24)، 48 (T48) و 72 (T72) ساعت ذخیره­سازی، سلامت غشای پلاسمایی، زنده­مانی (رنگ­آمیزی هوخست 33258) و تحرک اسپرم ارزیابی شد. در زمان 48 به‌منظور بررسی پراکسیداسیون چربی، غلظت مالون­دی­آلدهید (MDA) در یک میلیون  اسپرم اندازه­گیری شد. نتایج نشان داد، غلظت MDA در تیمار R50 (nM 93/0) کمتر از شاهد (nM15/1) و بیشتر از R40 (nM82/0) بود (05/0>P). سلامت غشای پلاسمایی و زنده‌مانی اسپرم در غلظت­های µg/mL 40 عصارۀ الکلی رزماری (به ترتیب 72/75درصد و 56/73درصد) از غلظت­های 0 (به­ترتیب 60/70درصد و  08/69درصد) و µg/mL 10 (به ترتیب 56/71درصد و72/69درصد ) عصاره بیشتر بود (05/0>P). اثر متقابل عصارۀ الکلی رزماری و زمان ذخیره­سازی بر تحرک پیش‌روندۀ اسپرم خروس معنی­دار بود (05/0>P). پس از 72 ساعت، تحرک پیش‌روندۀ اسپرم در تیمار R40 (46درصد)­ بیشتر از تیمارهای R0 (34درصد)، R10 (6/35درصد) و R20 (8/38درصد) بود (05/0>P). بنابراین عصارۀ الکلی رزماری سبب بهبود کیفیت اسپرم خروس طی ذخیره‌سازی در °C 4 شد.

کلیدواژه‌ها


عنوان مقاله [English]

Storage of rooster semen in liquid form using alcoholic extract of rosemary

نویسندگان [English]

  • Haniyeh Ramezaninejad 1
  • Mohammad Roostaei Ali Mehr 2
1 Former M. Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran
چکیده [English]

Current experiment was conducted to evaluate the effect of alcoholic extract of rosemary leaves on rooster sperm storage at 4 °C by using eight mature roosters. Semen collection was performed three days’ interval in 5 times. In each session, ejaculates were pooled, split into six parts and the amounts of 0 (R0), 10(R10), 20 (R20), 30 (R30), 40 (R40) and 50 µg/mL (R50) of rosemary extract were added to each part. After that, samples were chilled to 4 ℃ and kept until 72 h. Sperm viability (by staining Hoechst 33258), motility and functional membrane integrity were evaluated at 0 (T0), 24 (T24), 48 (T48) and 72 h (T72). Concentration of malondialdehyde (MDA) was determined to assay lipid peroxidation by one million spermatozoa at 48 h. The results showed that the concentration of MDA was lower in R50 (0.93 nM) than control (1.15 nM), but, it was higher than R40 (0.82 nM, P<0.05). Membrane integrity and sperm viability were higher in 40 µg/mL rosemary extract (75.72% and 73.56%, respectively) than control (70.6% and 69.08%, respectively) and 10 µg/mL extract (71.56% and 69.72%, respectively; P<0.05). There was interaction between rosemary extract and storage time on sperm motility (P<0.05). After 72 h, sperm motility was higher R40 (46%) than R0 (34%), R10 (35.6%) and R20 (38.8%, P<0.05). Therefore, rosemary extract improves quality of rooster spermatozoa during storage at 4 °C.

کلیدواژه‌ها [English]

  • Lipid Peroxidation
  • Rooster
  • Rosemary extract
  • Spermatozoa
  1. Aghaei, S., Nikzad, H., Taghizadeh, M., Tameh, A. A., Taherian, A. & Moravveji, A. (2014). Protective effect of Pumpkin seed extract on sperm characteristics, biochemical parameters and epididymal histology in adult male rats treated with Cyclophosphamide. Andrologia, 46, 927-928.
  2. Aitken, R. J. (1995). Free radicals, lipid peroxidation and sperm function. Reproduction, Fertility and Development, 7, 659-668.
  3. Aitken, R. J., Ryan, A. L., Baker, M. A. & Mclaughlin, E. A. (2004). Redox activity associated with the maturation and capacitation of mammalian spermatozoa. Free Radical Biology and Medicine, 36, 994-1010.
  4. Al-Daraji, H. J. (2012). Adding olive oil to rooster semen diluents for improving semen quality and storage ability during liquid storage. Baltic Journal of Comparative and Clinical Systems Biology, 42, 3-11.
  5. Allen, J. C. & Hamilton, R. J. (1989). Rancidity in Foods. (2nd ed.). New York.
  6. Bansal, A. K. & Bilaspuri, G. S. (2011) Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 2011, 1-7.
  7. Armstrong, J. S., Rajasekaran, M., Chamulitrat, W., Gatti, P., Hellstrom, W. J. & Sikka, S. C. (1999). Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Radical Biology and Medicine, 26, 869-880.
  8. Aurich, J. E., Schonherr, U., Hoppe, H. & Aurich, C., (1997). Effect of antioxidants on motility and membrane integrity of chilled-stored stallion semen. Theriogenology, 48,185-92.
  9. Blesbois, E., Grasseau I. & Seigneurin, F. (2005). Membrane fluidity and the ability of domestic bird spermatozoa to survive cryopreservation. Reproduction, 129, 371–378.
  10. Breque, C., Surai, P. & Brillard, J. P. (2003). Roles of antioxidants on prolonged storage of avian spermatozoa in vivo and in vitro. Molecular Reproduction and Development, 66, 314-323.
  11. Buckland, R. B., Scott, T. A. & Ansah, G. A. (1980) Genetic and environmental variation in semen production and fertility of chickens and turkeys. In: proceeding of the 9th congress on animal reproduction and artificial insemination, 16-20 Jun., Spanish Ministry of Agriculture, International Veterinary Association for Animal Production, Madrid. Spain, pp. 535-545.
  12. Buege, J. A. & Aust, S. D. (1978). Microsomal lipid peroxidation. Methods Enzymology, 52, 302-310.
  13. Burrows, W. H. & Quinn, J. P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poultry Science, 16, 19-24.
  14. Cao, G. & Cutler, R. G. (1993). High concentrations of antioxidants may not improve defense against oxidative stress. Archives Gerontology Geriatrics, 17, 189-201.
  15. Capucho, C., Sette, R., de Souza Predes, F., De Castro Monteiro, J., Pigoso, A. A., Barbieri, R., Dolder, M. A. & SeveriAguiar, G. D. (2012). Green Brazilian propolis effects on sperm count and epididymis morphology and oxidative stress. Food and Chemical Toxicology, 50, 3956-3962.
  16. Cecil, H. C. & Bakst, M. R. (1993). In vitro peroxidation of turkey spermatozoa. Poultry Science, 72, 1370-1378.
  17. Cerolini, S., Zainiboni, L., Maldjian, A. & Gliozzi, T. (2006). Effect of docosahexaenoic acid and α-tocopherol enrichment in chicken sperm on semen quality, sperm lipid composition and susceptibility to peroxidation. Theriogenology, 66, 877-886.
  18. Christova, Y., James, P. S. & Jones, R. (2004). Lipid diffusion in sperm plasma membranes exposed to peroxidative injury from oxygen free radicals. Molecular Reproduction and Development, 68, 365-372.
  19. Chung, L. Y., Cheung, T. C., Kong, S. K., Fung, K. P., Choy, Y. M., Chan, Z. H. & Kwok, T. T. (2001). Induction of apoptosis by green tea catechins in human prostate cancer DU145 cells. Life Sciences, 68, 1207-1214.
  20. Del Campo, J., Amiot, M. J. & Nguyen, C. (2000). Antimicrobial effect of rosemary extracts. Journal of Food Protection, 63, 1359-1368.
  21. Donoghue A.M. & Wishart, G.J. (2000). Storage of poultry semen. A review. Animal Reproduction Science, 62, 213-232.
  22. Erkan, N., Ayranci, G. & Ayranci, E. (2008). Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol. Food Chemistry, 110, 76-82.
  23. González, N., Gil, L., Martinez, F., Malo, C., Cano, R., Mur P. & Espinosa., E. (2010). Effect of natural antioxidant rosemary in canine soya freezing extender. Reproduction in Domestic Animal, 87-88.
  24. Guthrie, H.D. & Welch, G.R. (2012). Effects of reactive oxygen species on sperm function. Theriogenology, 78, 1700-1708.
  25. Huang, S.W., Frankel, K., Schwarz, R., Aeschbach R. & German, J.B. (1996). Anti-oxidant activity of carnosic acid and methyl carnosate in bulk oils and oil-in-water emultion. Journal of Agricultural and Food Chemistry, 44, 2951-2956.
  26. Khodaei Motlagh, M., Sharafi, M., Zhandi, M., Mohammadi-Sangcheshmeh, A., Shakeri, M., Soleimani M. & Zeinoaldini, S. (2014). Antioxidant effect of rosemary (Rosmarinus officinalis L.) extract in soybean lecithin-based semen extender following freeze–thawing process of ram sperm. Cryobiology, 69, 217-222.
  27. Kim, S. H., Yu, D. H. & Kim, Y. J. (2010). Effects of cryopreservation on phosphatidylserine translocation intracellular hydrogen peroxide, and DNA integrity in canine sperm. Theriogenology, 73, 282-292.
  28. Kumaresan, A., Kadirvel, G., Bujarbaruah, K.M., Bardoloi, R.K., Das, A., Kumar, S. & Naskar S. (2008) Preservation of boar semen at 18 ◦C induces lipidperoxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science, 110, 162-171
  29. Leeuw, A. M., Daas, G. H. J. & Woelders, H. (1991). The fix vital stain method simultaneous determination of viability and acrosomal and status of bovine spermatozoa. Journal of Andrology, 12, 112-118.
  30. Long, J.A. & Kramer, M. (2003). Effect of vitamin E on lipid peroxidation and fertility after artificial insemination with liquid-stored turkey semen. Poultry Science, 82, 1802-1807.
  31. Roostaei-Ali Mehr, M. & Adishi, B. (2015) Effect of Watercress extract on rooster semen during storage at 4°C.  Animal Production Research, 4, 15-24. (in Farsi)
  32. Malo, C., Gil, L., Cano, R., Martinez F. & Gale, I. (2011). Antioxidant effect of rosemary (Rosmarinus officinalis) on boar epididymal spermatozoa during cryopreservation. Theriogenology, 75, 1735-1741.
  33. Ommati, M.M., Zamiri, M.J., Akhlaghi, A., Atashi, H., Jafarzadeh, M.R., Rezvani, M.R. & Saemi, F. (2013). Seminal characteristics, sperm fatty acids, and blood bio-chemical attributes in breeder roosters orally administered with sage (Salvia officinalis) extract. Animal Production Science, 53, 548-554.
  34. Ozcan, M. (2003). Antioxidant activities of rosemary, sage, and sumac extracts and their combinations on stability of natural peanut oil. Journal of Medicinal Food, 6, 267-270.
  35. Saemi, F., Zamiri, M. J., Akhlaghi, A., Niakousari, M., Dadpasand, M. & Ommati, M. M. (2012). Dietary inclusion of dried tomato pomace improves the seminal characteristics in Iranian native roosters. Poultry Science, 91, 2310-2315.
  36. Sanocka, D. & Kurpisz, M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology, 2, 1-7. 
  37. Sarica S., Corduk, M., Suicmez, M., Cedden, F., Yildirim M. & Kilinc, K. (2007). The effects of dietary L-carnitine supplementation on semen traits, reproductive parameters, and testicular histology of Japanese quail breeders. The Journal of Applied Poultry Research, 16, 178-186.
  38. Sergedieneça, E., Jonssonb, K., Szymusiakc, H. & Tyrakowskac, B. (1999). Description of quantitative structure-activity relationships prooxidant toxicity of polyphenolic antioxidants to HL-60 cells. FEBS Letters, 462, 392-396.
  39. Shafigh, H., Shakeri, M., Zeinoaldini, S., Kohram, H., Zhandi, M. & Moghbeli, M. (2016) Improving sperm cryopreservation of rooster by using rosemary alcoholic essential oil. Journal Animal Production, 18, 615-624. (in Farsi)
  40. Slamenova, D., Horvathova, E., Kovacikova, Z., Kozics, K. & Hunakova, L. (2011). Essential rosemary oil protects testicular cells against DNA-damaging effects of H2O2 and DMNQ. Food Chemistry, 129, 64-70.
  41. Storey, B. T. (2008). Mammalian sperm metabolism: oxygen and sugar, friend and foe. International Journal of Developmental Biology, 52, 427-437.
  42. Syrai, P. E., Cerrolini, S., Wishart, G. J., Speake, B. K., Noble, R. C. & Sparks, N. H. C. (1998). Lipid and antioxidant composition of chicken semen and its susceptibility to peroxidation. Poultry Avian Biology, 9, 11-23.
  43. Tabatabaei, S., Batavani, R. & Ayen, E. (2011). Effects of Vitamin E addition to chicken semen on sperm quality during in vitro storage of semen. Veterinary Research Forum, 2, 103-111.
  44. Wierzbowski, S. & Kareta, W. (1993). An assessment of sperm motility estimation for evaluation in rams. Theriogenology, 40, 205-209.
  45. Zanganeh, Z., Zhandi, M., Zare-Shahneh, A., Najafi, A., Mahdi Nabi, M. & Mohammadi-Sangcheshmeh, A. (2013). Does rosemary aqueous extract improve buck semen cryopreservation? Small Ruminant Research, 114, 120-125.
  46. Zaniboni, L. & Cerolini, S. (2009). Liquid storage of turkey semen: Changes in quality parameters, lipid composition and susceptibility to induced in vitro peroxidation in control, n-3 fatty acids and alpha-tocopherol rich spermatozoa. Animal Reproduction Science, 112, 51-65.