ذخیره‌سازی منی خروس به‌صورت مایع با استفاده از عصارۀ الکلی رزماری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق کارشناسی ارشد، گروه علوم دامی، دانشکدة علوم کشاورزی، دانشگاه گیلان

2 دانشیار، گروه علوم دامی، دانشکدة علوم کشاورزی، دانشگاه گیلان

چکیده

این آزمایش برای بررسی عصارۀ الکلی برگ رزماری بر ذخیره­سازی اسپرم خروس در °C 4 آزمایشی با استفاده از هشت قطعه خروس بالغ انجام شد. گرد­آوری منی سه روز یک‌بار در پنج نوبت انجام شد. انزال­ها در هر نوبت پس از تجمیع به شش قسمت تقسیم و مقدار 0 (R0)، 10 (R10)،  20(R20)،30 (R40)، 40 و 50 (R50)  میکروگرم عصارۀ الکلی رزماری در میلی­لیتر به هر قسمت اضافه شد. سپس نمونه­ها تا °C 4 سرد شد و به­مدت 72 ساعت در این دما نگهداری شد. در زمان­های 0 (T0)، 24 (T24)، 48 (T48) و 72 (T72) ساعت ذخیره­سازی، سلامت غشای پلاسمایی، زنده­مانی (رنگ­آمیزی هوخست 33258) و تحرک اسپرم ارزیابی شد. در زمان 48 به‌منظور بررسی پراکسیداسیون چربی، غلظت مالون­دی­آلدهید (MDA) در یک میلیون  اسپرم اندازه­گیری شد. نتایج نشان داد، غلظت MDA در تیمار R50 (nM 93/0) کمتر از شاهد (nM15/1) و بیشتر از R40 (nM82/0) بود (05/0>P). سلامت غشای پلاسمایی و زنده‌مانی اسپرم در غلظت­های µg/mL 40 عصارۀ الکلی رزماری (به ترتیب 72/75درصد و 56/73درصد) از غلظت­های 0 (به­ترتیب 60/70درصد و  08/69درصد) و µg/mL 10 (به ترتیب 56/71درصد و72/69درصد ) عصاره بیشتر بود (05/0>P). اثر متقابل عصارۀ الکلی رزماری و زمان ذخیره­سازی بر تحرک پیش‌روندۀ اسپرم خروس معنی­دار بود (05/0>P). پس از 72 ساعت، تحرک پیش‌روندۀ اسپرم در تیمار R40 (46درصد)­ بیشتر از تیمارهای R0 (34درصد)، R10 (6/35درصد) و R20 (8/38درصد) بود (05/0>P). بنابراین عصارۀ الکلی رزماری سبب بهبود کیفیت اسپرم خروس طی ذخیره‌سازی در °C 4 شد.

کلیدواژه‌ها

عنوان مقاله [English]

Storage of rooster semen in liquid form using alcoholic extract of rosemary

نویسندگان [English]

  • Haniyeh Ramezaninejad 1
  • Mohammad Roostaei Ali Mehr 2
1 Former M. Sc. Student, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, University of Guilan, Iran
چکیده [English]

Current experiment was conducted to evaluate the effect of alcoholic extract of rosemary leaves on rooster sperm storage at 4 °C by using eight mature roosters. Semen collection was performed three days’ interval in 5 times. In each session, ejaculates were pooled, split into six parts and the amounts of 0 (R0), 10(R10), 20 (R20), 30 (R30), 40 (R40) and 50 µg/mL (R50) of rosemary extract were added to each part. After that, samples were chilled to 4 ℃ and kept until 72 h. Sperm viability (by staining Hoechst 33258), motility and functional membrane integrity were evaluated at 0 (T0), 24 (T24), 48 (T48) and 72 h (T72). Concentration of malondialdehyde (MDA) was determined to assay lipid peroxidation by one million spermatozoa at 48 h. The results showed that the concentration of MDA was lower in R50 (0.93 nM) than control (1.15 nM), but, it was higher than R40 (0.82 nM, P<0.05). Membrane integrity and sperm viability were higher in 40 µg/mL rosemary extract (75.72% and 73.56%, respectively) than control (70.6% and 69.08%, respectively) and 10 µg/mL extract (71.56% and 69.72%, respectively; P<0.05). There was interaction between rosemary extract and storage time on sperm motility (P<0.05). After 72 h, sperm motility was higher R40 (46%) than R0 (34%), R10 (35.6%) and R20 (38.8%, P<0.05). Therefore, rosemary extract improves quality of rooster spermatozoa during storage at 4 °C.

کلیدواژه‌ها [English]

  • Lipid Peroxidation
  • Rooster
  • Rosemary extract
  • Spermatozoa

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  • تاریخ دریافت: 15 آذر 1395
  • تاریخ بازنگری: 03 اسفند 1395
  • تاریخ پذیرش: 03 اسفند 1395

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