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بررسی چندریختی اگزون چهار ژن هورمون رشد و اگزون ده گیرنده هورمون رشد و ارتباط آن‌ها با صفات لاشه در گوسفند نژاد لری بختیاری

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، گروه علوم دامی، دانشکدة مهندسی علوم زراعی و دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانش‌آموختة کارشناسی ارشد گروه علوم دامی، دانشکدة کشاورزی دانشگاه کرمان

3 دانشیار، گروه علوم دامی، دانشکدة مهندسی علوم زراعی و دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 کارشناس ارشد و مسئول بخش دام سبک، مرکز اصلاح‌نژاد و بهبود تولیدات دامی کشور

چکیده

ژن هورمون رشد ((GH ژنی نامزد (کاندیدا) برای رشد حیوانات اهلی است و هورمون رشد نقش مهمی در سوخت‌وساز (متابولیسم) رشد دارد. برای اینکه هورمون رشد بتواند در سوخت‌وساز رشد شرکت کند نیاز به گیرنده­های آن (GHR) در سطح یاخته­های هدف دارد. این پژوهش با هدف بررسی ارتباط چندریختی ژن­های GH و GHR با صفات لاشه در نژاد لری بختیاری انجام شد. برای این منظور، از سیاهرگ وداج تعداد 152 رأس گوسفند نژاد لری بختیاری خون‌گیری شد. استخراج DNA از خون با روش استخراج نمکی بهینه یافته انجام و واکنش زنجیره­ای پلیمراز برای افزایش قطعه­های 214 جفت­بازی از اگزون چهار ژن هورمون رشد و 218 جفت­بازی از اگزون ده ژن گیرندة هورمون رشد انجام شد. پس از تعیین چندریختی فضایی تک­رشته­ای محصولات PCR، الگوهای باندی مربوط به ژن­های GH و GHR روی ژل پلی اکریل­آمید و رنگ‌آمیزی با نیترات نقره، مشاهده شد. نتایج بیانگر چندریختی در جایگاه ژن GH بود، اما برای ژن GHR، هیچ‌گونه تفاوتی بین باندها مشاهده نشد. نتایج توالی­یابی منجر به شناسایی پنج چندریختی تک نوکلئوتیدی برای ژن GH در جمعیت مورد بررسی شد. هیچ‌یک از ژنوتیپ­های ژن GH با صفات مورد بررسی ارتباط معنی­داری نشان ندادند.

کلیدواژه‌ها


عنوان مقاله [English]

Study the polymorphism of Exon 4 of GH gene and exon 10 of GHR gene and their association with carcass traits in Lori-Bakhtiari breed sheep using PCR-SSCP

نویسندگان [English]

  • Hossein Moradi Shahrbabak 1
  • Rasoul Khodabakhshzadeh 2
  • Mostafa Sadeghi 3
  • Amir TaheriYeganeh 4
1 Assistant Professors, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Fromer M.Sc. Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 Former M. Sc. Student and Head of Animal Breeding Center of Ministry of Agriculture of IRAN
چکیده [English]

The growth hormone (GH) gene is a candidate for growth trait in farm animals and plays an important role in growth metabolism. Growth hormone can contribute in growth metabolism when it's receptor (GHR) are on cells target. The single nucleotide polymorphism was occurred in GH and GHR genes and that associated with carcass traits in Lori-Bakhtiari sheep breed with using PCR-SSCP. In this research, blood samples were collected from the left jugular vein from 152 Lori-Bakhtiari sheep breed. Genomic DNA was extracted from blood samples by using the salting-out procedure and PCR were used to amplify the regions are located in exon 4 (214 bp segments) of the ovine GH gene and exon 10 (218 bp segments) of the ovine GHR Gene. The single stranded conformation polymorphism (SSCP) patterns of PCR products were studied using acrylamide gel electrophoresis and silver-nitrate staining method. The result showed polymorphism in GH gene but any difference between banding patterns for GHR gene was not observed. The sequencing results showed the presence of 5 Single nucleotide polymorphism for GH gene in the studied population. No significant associations of the available genotypes in the exon 4 of the ovine GH Gene with carcass traits.

کلیدواژه‌ها [English]

  • Growth hormone gene
  • growth hormone receptor gene
  • Single nucleotide polymorphism
  • sequencing
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