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تأثیر تزریق درون آمنیوتیکی متیونین- روی و نانو متیونین- روی بر بیان ژن متالوتیونین در جوجه‌های گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه گیلان، رشت، ایران

2 دانشیار، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه گیلان، رشت، ایران

3 استادیار، گروه علوم دامی، دانشکدة کشاورزی، دانشگاه گیلان، رشت، ایران

چکیده

تزریق درون آمنیوتیکی متیونین- روی و نانو متیونین- روی، بر میزان بیان mRNA متالوتیونین در کبد و رودة جوجه­های گوشتی بررسی شد. به این منظور شمار ۴۰۰ عدد تخم­مرغ بارور(سویة راس ۳۰۸) در قالب طرح کامل تصادفی به چهار تیمار، چهار تکرار و ۲۵ عدد تخم­مرغ در هر تکرار تقسیم شدند. تیمارهای آزمایشی شامل ۱- بدون تزریق (کنترل منفی)، ۲- تزریق 1 میلی­لیتر محلول نمک 9/0 درصد (کنترل مثبت)، ۳- تزریق 1 میلی­لیتر محلول متیونین- روی و ۴- تزریق 1 میلی­لیتر محلول نانو متیونین- روی بودند. تزریق در روز ۱۷ جوجه­کشی و در مایع آمنیوتیک تخم­مرغ­ها انجام شد. نتایج نشان داد، تیمارها تأثیر معنی­داری بر درصد جوجه‌درآوری نداشتند (05/0P>). تزریق متیونین- روی و نانو متیونین- روی باعث افزایش معنی‌دار (01/0P˂) وزن جوجه­ها در زمان تفریخ و تا سن سه هفتگی شده، وزن کبد و روده در زمان تفریخ و در سن یک هفتگی به‌صورت معنی­دار (01/0P˂) افزایش یافت. در مقایسه با تیمارهای کنترل، بیان mRNAی متالوتیونین کبد و روده در نتیجه تزریق متیونین– روی و نانو متیونین– روی، افزایش معنی­داری (01/0P˂) داشته و این اختلاف در سن یک ­هفتگی ادامه یافت. نتیجه‌گیری نهایی این است، تزریق نانو متیونین – روی و متیونین– روی به­عنوان مکمل تغذیه‌ای جنینی سبب افزایش وزن جوجه­های گوشتی در سه هفتة اول پرورش می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Intra Amniotic Injection of Zinc-Methionine and Nano Zinc-Methionine on Metallothionein Gene Expression in the Broiler chickens

نویسندگان [English]

  • Kolsoom Razani 1
  • Majid Mottaghitalab 2
  • Seyed Hosein Heseini Moghaddam 3
1 Ph.D. Student, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 Assistant Professor, Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
چکیده [English]

The effects of intra amniotic injection of zinc-methionine and nano zinc-methionine on metallothionein mRNA expression of chicken’s liver and intestine were studied. Four hundred fertiled eggs (Ross 308 strain) were allocated to 4 treatments, 4 replicates/ treatment and 25 eggs per replicate using a CRD (Complete Random Design) design. Experimental treatments were: 1- no injection (negative control), 2- injection of 1 ml 0.9% saline solution (positive control), 3- injection of 1 ml 25% zinc- methionine solution and 4- injection of 1 ml 25% nano-zinc- methionine solution. On day 17 of incubation, eggs were injected using insulin syringes. Results showed that in Ovo injection lead to no significant effect on hatchability. The injection of zinc-methionine and nano zinc-methionine caused significant (P<0.01) increase in chicks weight at hatch, and a week after that. A similar result was obtained for liver and intestine samples. Groups treated with zinc-methionine and nano zinc-methionine showed significant (P<0.01) increase in metallothionein mRNA expression in liver and intestine and remained significant (P<0.01) at day 7 after hatch. Experimental treatments significantly (P<0.01) increased the liver and intestine metallothionein content at day 1 and 7 after hatch. Conclusion is that in ovo injection of zinc-methionine and nano zinc-methionine lead to increase body weight at first 3weeks of broiler rearing.

کلیدواژه‌ها [English]

  • broiler
  • In Ovo feeding
  • metallothionein
  • nano zinc methionine
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