شناسایی چندشکلی تک نوکلئوتیدی در ژن گیرندة ملانوکورتین-1 (MC1R) در گوسفند لری بختیاری

نوع مقاله: مقاله پژوهشی


1 دانشجوی کارشناسی ارشد اصلاح نژاد دام، دانشکده کشاورزی، گروه علوم دامی دانشگاه یاسوج

2 استادیار ژنتیک و اصلاح دام، دانشکدة کشاورزی، دانشگاه یاسوج


گیرندة ملانوکورتین-1 که توسط جایگاه گسترش کد می‌شود، نقش مهمی در تنوع رنگ پوشش در دام‌های اهلی بازی می‌کند. در این بررسی، قطعه‌ای به طول840 جفت باز از ناحیة 5َ کناری و قسمت اعظم توالی کدکنندة ژن MC1R گوسفندی در پانزده رأس گوسفند لری بختیاری با فنوتیپ متفاوت تعیین توالی شد. نتایج تعیین توالی شمار هشت چندشکلی تک نوکلئوتیدی را نشان داد که شامل پنج جهش هم‌معنی (c.464G.A, c.557G>C, c.635c>T, c.692C>T and c.932C>T) و سه جهش غیر هم‌معنی (c.128G>C, p.Phe>Tyr, c.638G>A, p.Arg>Gln and c.653G>A, p.Arg>His) بودند. بر پایة نتایج به‌دست‌آمده، در میان سه جهش دوم، جهش c.128G>C که منجر به تغییر اسید آمینة فنیل آلانین به تیروزین در برگیرندة ملانوکورتین-1 شده می‌تواند بر عملکرد پروتئین مورد نظر نقش مهمی داشته باشد. افزون بر این، این چندشکلی در جایگاه 128 تنها در یکی از فنوتیپ‌ها مشاهده شد. اگرچه، دو جهش دیگر در جایگاه‌های 638 و 653 منجر به تغییر اسیدآمینه شدند ولی این جهش‌ها تأثیری بر عملکرد پروتئین مربوطه نداشتند. بنابراین، نتایج پیشنهاد می‌کند که جهش‌های ژن MC1R در گوسفند می‌تواند با رنگ پوشش در گوسفند لری بختیاری در ارتباط باشد.


عنوان مقاله [English]

Identification of single nucleotide polymorphisms (SNPs) in the ovine melanocortin-1 receptor (MC1R) gene in Lori-Bakhtiari sheep

نویسندگان [English]

  • Mehdi Abedi Dehsheikh 1
  • Mustafa Muhaghegh Dolatabady 2
1 M.Sc. Student, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
چکیده [English]

The melanocortin receptor 1 (MC1R), encoded by extension locus, plays an important role in coat color variation in livestock.  In this study, the 840 bp of 5′- flanking region and most part of coding sequence of ovine MC1R gene was sequenced in 15 Lori-Bakhtiari sheep with different coat colors. The results showed eight single nucleotide polymorphisms (SNPs): five synonymous mutations (c.464G.A, c.557G>C, c.635c>T, c.692C>T and c.932C>T) and three non-synonymous mutations (c.128G>C, p.Phe>Tyr, c.638G>A, p.Arg>Gln and c.653G>A, p.Arg>His). Based on the results, among the three non-synonymous mutations, the c.128G>C mutation which resulting in the replacement of Phe with Tyr amino acid could affect the functional performance of MC1R protein and this substitution might cause the color variation in this breed. In addition, the c.128G>C was observed in single phenotype. However, the two SNPs at position 638 and 653 led to substitution of two amino acids in MC1R, but these mutations didnot influence on MC1R performance. Therefore, our results suggest that the mutations of ovine MC1R gene could be associated with coat color phenotype in Lori-Bakhtiari sheep.

کلیدواژه‌ها [English]

  • Coat color
  • Lori-Bakhtiari
  • MC1R gene
  • sheep
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