شناسایی miRNAها و ایزومیرهای جدید در بافت کبد گاوهای شیری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دام و طیور، پردیس ابوریحان دانشگاه تهران

2 استادیار، گروه علوم دام و طیور، پردیس ابوریحان دانشگاه تهران

3 دانشیار، گروه علوم دام و طیور، پردیس ابوریحان دانشگاه تهران

چکیده

تعادل منفی انرژی (NEB) در گاوهای شیری پرتولید در چند هفتة نخست پس از زایمان رخ می­دهد و به دلیل اثرگذاری منفی بر باروری و سلامتی اهمیت اقتصادی زیادی در گله­های گاو شیری دارد. بنابراین، شناسایی هر چه بهتر سازوکارهای تنظیمی مؤثر در این اختلال سوخت‌وسازی (متابولیکی) اهمیت دارد. یکی از عامل‌های تنظیمی مؤثر در NEB، miRNAها هستند. به‌رغم اهمیت NEB، سازوکارهای تنظیمی مربوط به miRNAها در این دوره به‌خوبی شناخته نشده­اند. در این بررسی داده­های miRNA-seq مربوط به بافت کبد هشت گاو شیری هلشتاین موجود در بخش GEO بانک اطلاعاتی NCBI برای شناسایی miRNAها و ایزومیرهای جدید تجزیه‌وتحلیل شدند. در مجموع، 291 miRNA جدید که ژن همتا (همولوگ) در دیگر گونه­ها داشتند، و 164 miRNA جدید بدون همتا شناسایی شد. بررسی عملکرد ژن­های هدف miRNAهای شناسایی‌شده نشان داد، این ژن­ها در مسیرهای زیستی (بیولوژیکی) مرتبط با NEB نقش دارند. افزون بر این 446 ایزومیر و 95 miRNA* جدید برای نخستین بار در ژنگان (ژنوم) گاو گزارش شد. یافته‌های به‌دست‌آمده از این بررسی اطلاعات جدیدی برای درک بهتر نقش تنظیمی miRNAها در NEB فراهم می­کند.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of new miRNAs and isomirs in liver tissue of dairy cows

نویسندگان [English]

  • Zohre Mozduri 1
  • Mohammad Reza Bakhtiarizadeh 2
  • Abdolreza Salehi 3
1 M. Sc. Student, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran
2 Assistant Professor, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran
3 Associate Professor, Department of Animal and Poultry Sciences, Aburaihan Campus, University of Tehran, Iran
چکیده [English]

Negative energy balance (NEB) occurs inhigh-producing dairy cows in first few weeks after parturition, that energy demand for maintenance and milk production exceeds the dietary energy intake. NEB has a considerable economic importance due to negative effect on health and fertility in dairy herds, therefore, the identification of its effective regulatory mechanism is important. miRNAs are one of these effective regulatory factors in NEB. Despite of the importance of NEB, the regulatory mechanisms related to miRNAs has not been well documented. In this study miRNA-seq data from liver tissue of eight Holstein dairy cows were analyzed to identify new miRNAs and isomirs. All data have been achieved from GEO in NCBI database. A total of 291 new miRNAs with homologous gene in other species were identified. Moreover, 164 new miRNAs without homologous were identified. Investigation of target genes of these miRNAs lead to identify biological paths related to NEB. Also 466 new isomiR and 95 new miRNA* were detected for the first time in cow genome. The results of the current study provide new information for better understanding of the regulatory roles of miRNAs in NEB.

کلیدواژه‌ها [English]

  • IsomiR
  • negative energy balance
  • liver tissue

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  • تاریخ دریافت: 19 اسفند 1394
  • تاریخ بازنگری: 14 شهریور 1395
  • تاریخ پذیرش: 11 اسفند 1395

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