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تعیین جایگاه‌های تحت انتخاب مثبت در نژادهای گوسفند ایرانی بلوچی و زل

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، دانشکدۀ علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

2 دانشیار، گروه علوم دامی، دانشکدۀ علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استادیار، دانشکدۀ کشاورزی و منابع طبیعی دانشگاه اراک

4 استادیار، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

نشانه­های انتخاب در کل ژنگان (ژنوم)، ما را در درک سازوکار­های انتخاب و شناسایی مناطقی از ژنگان که در طی سالیان متمادی به‌صورت طبیعی و یا مصنوعی انتخاب شده­اند، راهنمایی می­کنند. هدف این تحقیق شناسایی نقاطی از ژنگان در گوسفندان زل و بلوچی بود که در طی سال­های مختلف به‌صورت مصنوعی یا طبیعی انتخاب شده­اند. 143 رأس گوسفند بلوچی (96 رأس) و زل (47 رأس)، با استفاده از آرایه­های ژنگانیIllumina ovine SNP50K BeadChip  تعیین ژنوتیپ شدند. برای جستجوی نشانه­های انتخاب از آزمون نااُریب FST ویر و کوکرهام (تتا) در بستۀ نرم‌افزاری R استفاده شد. نتایج 17 منطقۀ ژنگانی روی کروموزوم­های 3، 4، 5، 7، 10، 11، 12، 13، 15، 18 و X را شناسایی کرد. تجزیه‌وتحلیل‌ اطلاعات زیستی (بیوانفورماتیکی) نشان داد که برخی از این مناطق ژنگانی با ژن­های مؤثر بر صفات گسترش نظام استخوان‌بندی (اسکلتی) و دم، ایمنی و یاخته‌شناختی (سیتولوژی) یاخته‌ای، سوخت‌و‌ساز (متابولیسم) قند و انرژی و تولید­مثلی مانند ژن­های RPS6KA3، HOXB9، ESPL1، AAAS، FNDC3A همپوشانی دارند. نتایج این تحقیق و جایگاه­های ژنگانی شناسایی‌شده می­تواند نقش مهمی در رابطه با بررسی تأثیر انتخاب در تمایز جمعیتی دو نژاد دنبه‌دار بلوچی و بدون دنبۀ زل و در پی آن شناسایی مناطق ژنگانی مرتبط با صفات متمایزکنندۀ این دو نژاد داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Detection of loci under positive selection in Iranian Baluchi and Zel sheep breeds

نویسندگان [English]

  • Zeinab Manzari 1
  • Hassan Mehrabani Yeghaneh 2
  • Ardeshir Najati-Javaremi 2
  • Mohammad Hossein Moradi 3
  • Mohsen Gholizadeh 4
1 M. Sc. Student, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Assistant Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Arak, Iran
4 Assistant Professor, Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, Iran
چکیده [English]

Selective signatures in whole genome can help us to understand the mechanisms of selection and to identify the genomic regions that have been under natural or artificial selection during long years. The objective of this study was to identify the genomic regions that have been under artificial and natural selection in Baluchi and Zel sheep breeds. 143 sheep from Baluchi (N=96) and Zel breeds (N=47) have been genotyped using the Illumina ovine SNP50 BeadChip. Unbiased method of Weir and Cockerham’s FST (Theta) was used to detect the selection signatures in the R package. The results revealed seventeen genomic regions on 3, 4, 5, 7, 10, 11, 12, 13, 15, 18 and X chromosomes. Bioinformatics analysis demonstrated that some of these genomic regions overlapped with reported genes included in the development of the skeletal system and tail, cytology cells, immune system, sugar and energy metabolism and reproduction traits such as RPS6KA3, HOXB9, ESPL1, AAAS, FNDC3A genes. The results of the present study and the identified genomic regions can play an important role in study of the effect of the selection on population differentiation in two Baluchi fat-tailed and Zel thin-tailed breeds and subsequently, would direct us to identify the genomic regions associated with traits differentiate these breeds.

کلیدواژه‌ها [English]

  • Baluchi sheep
  • candidate genes
  • Positive selection
  • unbiased method of Weir and Cockerham’s FST
  • Zel sheep
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