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همراهی همردیف‌های ژن BoLA-DRB3 با افزایش یاخته‌های تک‌هسته‌ای خون محیطی در پاسخ به استافیلوکوکوس آرئوس

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج و دانشیار گروه علوم دامی، دانشگاه شیراز

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

4 4- استاد گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی ساری

چکیده

هدف این پژوهش، بررسی همراهی همردیف (آلل)­های ژن BoLA-DRB3 با افزایش یاخته­های تک­هسته­ای خون محیطی در پاسخ به استافیلوکوکوس آرئوس بود. حیوانات موردبررسی (347 حیوان) شامل 155 گوسالۀ آمیختۀ هلشتاین-شاروله، 60 گوسالۀ ناشی از آمیزش حیوانات نر هلشتاین- شاروله با حیوانات مادۀ هلشتاین، 46 گوسالۀ ناشی از آمیزش حیوانات مادۀ هلشتاین- شاروله با حیوانات نر شاروله و 86 گوسالۀ هلشتاین بودند. همۀ حیوانات در هنگام آزمایش هم­سن و همۀ تلیسه­ها غیر آبستن بودند. برای تعیین ژنوتیپ حیوانات در جایگاه BoLA-DRB3 روش توالی­یابی مستقیم استفاده شد. با استفاده از رویۀ REML و مدل­های خطی مختلط همراهی همردیف­های ژن BoLA-DRB3 با افزایش یاخته­های تک­هسته­ای خون محیطی ارزیابی شد. افزون بر تأثیر ژن BoLA-DRB3، عامل‌های ثابت گروه ژنتیکی و جنس و عامل تصادفی پدر نیز در مدل گنجانده شد. در این پژوهش، 27 همردیف برای ژن BoLA-DRB3 دیده شد. نتایج نشان داد همردیف­های DRB3*0101 و DRB3*0902 با شاخص تحریک استافیلوکوکوس آرئوس همراهی دارند (05/0P<). یافته­های این پژوهش می­تواند برای شناخت سازوکار زیست‌شناختی (بیولوژیکی) پاسخ ایمنی میزبان علیه استافیلوکوکوس آرئوس سودمند باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Association of the BoLA-DRB3 alleles with peripheral blood mononuclear cell proliferation in response to Staphylococcusaureus

نویسندگان [English]

  • Hadi Atashi 1
  • Mohammad Moradi Shar e Babak 2
  • Hassan Mehrabani-Yegane 3
  • Seyed Reza Miraei-Ashtiani 2
  • Ghodratollah Rahimi-Mianji 4
1 Former Ph.D. Student, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran and Associate Professor, Department of Animal Science, University of Shiraz, Iran
2 Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The aim of this study was to investigate the association of bovine leukocyte antigen-DRB3 alleles with peripheral blood mononuclear cell (PBMC) proliferation in response to Staphylococcus aureus. The animals included in this study (n=347) were approximately of same age and comprised of F2 Holstein-Friesian ´ Charolais (n = 155), Holstein-Friesian backcross (F0 Holstein-Friesian dams crossed with unrelated F1 sires, n = 60), Charolais backcross (F1 dams crossed with F0 Charolais sire, n=46) and pure Holstein-Friesian (n = 86).  A sequence-based typing method was used in order to determine the genotype of the animals at BoLA-DRB3 locus and a linear mixed model was used to evaluate the association of bovine leukocyte antigen-DRB3 alleles with peripheral blood mononuclear cell (PBMC) proliferation. Beside the BoLA-DRB3 alleles, fixed effects of genetic group and gender and random effect of sires were included into the statistical model. In this research, 27 alleles were found for BoLA-DRB3 gene. The results showed that alleles BoLA-DRB3*0101 and BoLA-DRB3*0902 significantly affected on the stimulation index of S. aureus–induced peripheral blood mononuclear cell proliferation (P<0.05). The results may be useful for investigating the biological mechanism of immune response against S. aureus.

کلیدواژه‌ها [English]

  • BoLA-DRB3
  • Mastitis
  • peripheral blood mononuclear cell proliferation
  • Staphylococcus aureus
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