8954856055505db

شناسایی یک چندشکلی جدید در راه‌انداز ژن عامل رشد شبه انسولین-1 (igf1) در بلدرچین ژاپنی به روش PCR-SSCP

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، دانشکدۀ کشاورزی، دانشگاه صنعتی اصفهان

4 استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران

5 استاد، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

ژن عامل رشد شبه انسولین-1 (igf1) یک ژن کاندیدا برای بررسی صفات مرتبط با رشد، وزن بدن و رشد عضله در گونه­های مختلف است. به‌منظور بررسی ارتباط این ژن با صفات رشد در بلدرچین، هشت جفت بلدرچین سفید و وحشی به‌عنوان جمعیت صفر برای تولید افراد نسل اول (f1) با یکدیگر تلاقی داده شدند. از تلاقی بین 34 قطعه بلدرچین در نسل F1، 422 قطعه بلدرچین در نسل دوّم f2)) ایجاد شد. رکوردهای مربوط به وزن بدن در سنین مختلف در نسل دوّم ثبت و ارتباط بین چندشکلی بخشی از جایگاه راه‌انداز (پروموتور) ژن IGF1 و این رکوردها بررسی شد. با استفاده از روش PCR-SSCP یک چندشکلی تک نوکلئوتیدیG
15 → '> a  در ناحیة موردنظر ژن IGF1 در 472 قطعه بلدرچین در سه نسل تشخیص داده شد. فراوانی­ ژنوتیپ­های AA، AG و GG به ترتیب 05/0، 62/0 و 33/0 و فراوانی همردیف­ (آلل­)های ژنی A و G به ترتیب 36/0 و 64/0 محاسبه شد. وزن بدن در سن 1 تا 5 هفتگی و زمان کشتار و همچنین میانگین افزایش وزن روزانه از هفتۀ اول تا چهارم پرورش در ژنوتیپ AA، کمتر از ژنوتیپ AG و GG بود و این تفاوت ازنظر آماری معنی­دار نبود. میانگین افزایش وزن روزانة ماده­ها در ژنوتیپ AG بیشتر از نرها بود (001/0P<). نتایج ارائه‌شده در این نوشتار در مقایسه با گزارش‌های دیگر در مرغ، نشان‌دهندۀ یک چندشکلی جدید در ناحیۀ راه‌انداز ژن IGF1 در بلدرچین بود، اما ارتباط آن با صفات مرتبط با وزن بدن و نرخ رشد ازلحاظ آماری معنی­دار نبود.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of novel SNP in promoter of Insulin-Like Growth Factor-I (IGF1) gene in Japanese quail by PCR-SSCP assay

نویسندگان [English]

  • Homa Aarabi 1
  • Mohammad Moradi Shahrbabak 2
  • Abbas Pakdel 3
  • Hossein Moradi Shahrbabak 4
  • Ali Esmailizadeh koshkoiyeh 5
1 Former Ph.D. Student, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Faculty of Agriculture, University of Isfahan, Iran
4 Assistant Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
5 Professor, Faculty of agriculture, Shahid Bahonar University of Kerman, Iran
چکیده [English]

Insulin-like growth factor-1 gene (IGF1) is a biological candidate gene for investigation traits such as growth, body weight and muscles growth in different species. In this research eight pairs of white and wild quails were crossed reciprocally as a base population. A total of 34 quails were produced in first generation (F1) and 422 quails were generated by intercrossing the F1 population. Body weights at the time of hatching and different weeks were recorded in the second generation. The association between IGF1 promoter region polymorphism and body weight in different ages was investigated in F2 generation. Using PCR-SSCP assay and sequencing, a novel single nucleotide polymorphism (SNP) was identified in IGF1 promoter region in 472 birds from three Generations. Genotypic frequencies of AA, AG and GG genotypes were 0.05, 0.62 and 0.33, respectively for all generations. The frequency of A and G alleles were 0.36 and 0.64, respectively. The AA genotype was lower than AG and GG genotypes for body weights in ages of one to five weeks and slaughter time and the average of daily gain was also lower from week first to four but these differences weren’t significant. The average of daily gain was higher in females compared to males for AG genotype (P<0.001). Comparison between detected allele in the present study with reported allele by other research groups in chickens revealed a novel SNP in promoter of IGF1 gene in Japanese quail, but association between this polymorphism with body weight and growth rate were not significant.

کلیدواژه‌ها [English]

  • growth traits
  • Insulin-Like Growth Factor-I
  • Japanese quail
  • PCR-SSCP assay
  • sequencing
  1. Amills, M., Jimenez, N., Villalba, D., Tor, M., Molina, E., Cubilo, D., Marcos, C., Francesch, A., Sanchez, A. & Estany, M. (2003). Identification of three single nucleotide polymorphisms in the chicken insulin-like growth factor 1 and 2 genes and their associations with growth and feeding traits. Poultry Science, 82, 1485-1493.
  2. Arabi, H., Moradi Shahrbabak, M. & Nejati Javaremi, A. (2010). Divergent selection for four and five weeks body weight in Japanese quail. Iranian Journal ofAnimalScience, 4, 373-380. (in Farsi)
  3. Ayatollahi Mehrgardi, A. (2008). Selection for growth traits and reproduction in Japanese quail. Ph.D. Thesis. Faculty of Tehran University, Iran. (in Farsi)
  4. Baker, R.L., Peterson, A.J., Bass, J.J., Amyes, N.C., Breier, B.H. & Gluckman, P.D. (1991). Replicated selection for insulin-like growth factor-1 and body weight in mice. Theoretical and Applied Genetics, 81, 685-692.
  5. Beccavin, C., Chevalier, B., Cogburn, L.A., Simon, J. & Duclos, M.J. (2001). Insulin-like growth factors and body growth in chickens divergently selected for high or low growth rate. Journal of Endocrinology, 168, 297-306.
  6. Bowcock, A. & Sartorelli, V. (1990). Polymorphism and mapping of the IGF1 gene, and absence of association with stature among African pygmies. Human Genetics, 85, 349-54.
  7. Duclos, M.J., Beccavin, C. & Simon, J. (1999). Genetic models for the study of insulin-like growth factors (IGF) and muscle development in birds compared with mammals. Domestic Animal Endocrinology, 17, 231-243.
  8. El-Tarabany, M.S., Awad, A. & El-Bayomi, K.M. (2014). Genetic polymorphism of prolactin, bone morphogenetic protein receptor 1B and Insulin- like growth factor 1 genes in two selected lines of Japanese quail. Life Science Journal, 11(6), 408-416.
  9. Florini, J.R., Ewton, D.Z. & Coolican, S.A. (1996). Growth hormone and the insulin-like growth factor system in myo- genesis. Nature Reviews Endocrinology, 17, 481-517.
  10. Ikeobi, C.O., Woolliams, J.A., Morrice, D.R., Law, A., Windsor, D., Burt, D.W. & Hocking, P.M. (2002). Quantitative trait loci affecting fatness in the chicken. Animal Genetics, 33, 428-435.
  11. Kadlec, J., Hosnedlova, B., Rehout, V., Citek, J. & Vecerek, L. (2011). Insulin-like growth factor-I gene polymorphism and its association with growth and slaughter characteristics in broiler chickens. Journal of Agrobiology, 28(2), 157-163.
  12. Kajimoto, Y. & Rotwein, P. (1991). Structure of the chicken insulin-like growth factor I gene reveals conserved promoter elements. The American Society for Biochemistry and Molecular Biology, 266, 9724-9731.
  13. Kan, X.Z., Li, X.F., Lei, Z.P., Chen, L., Gao, H., Yang, Z.Y. & et al. (2010). Estimation of divergence times for major lineages of galliform birds: evidence from complete mitochondrial genome sequences. African Journal of Biotechnology, 9, 3073-3078.
  14. Khaldari, M. (2012). Statistical methods. (1st ed.). Jahad daneshgahi publication, Tehran. (in Farsi)
  15. Kayang, B.B., Vignal, A., Inoue-Murayama, M., Miwa, M., Monvoisin, J.L., Ito, S. & Minvielle, F. (2004). A first-generation microsatellite linkage map of the Japanese quail. Animal Genetics, 35, 195-200.
  16. Khadem, A., Hafezian, H. & Rahimi-Mianji, G.H. (2010). Association of single nucleotide polymorphisms in IGF- I, IGF-II and IGFBP-II with production traits in breeder hens of Mazandaran native fowls breeding station. African Journal of Biotechnology, 9(6), 805-810.
  17. Laere, A.V., Nguyen, M., Braunschweig, M., Nezer, C., Collette, C., Moreau, L., Archibald, A.L., Haley, C.S., Buys, N. & et al. (2003). A regulatory mutation in IGF2 causes a major QTL effect on muscle growth in the pig. Nature. 425, 832-836.
  18. Lei, M.M., Nie, Q.H., Peng, X., Zhang, D.X. & Zhang, Q. (2005). Single nucleotide polymorphisms of the chicken insulin-like factor binding protein 2 gene associated with chicken growth and carcass traits. Poultry Science, 84, 1191-1198.
  19. Lei, M., Luo, C., Peng, X., Fang, M., Nie, Q., Zhang, D., Yang, G. & Zhang, X. (2007). Polymorphism of growth-correlated genes associated with fatness and muscle fiber traits in chickens. Poultry Science, 86, 835-842.
  20. McMurtry, J.P., Francis, G.L. & Upton, Z. (1997). Insulin-like growth factors in poultry. Domestic Animal Endocrinology, 14, 199-229.
  21. Minvielle, F. (2004). The future of Japanese quail for research and production. World's Poultry Science Journal, 60, 500-507.
  22. Moe, H.H., Shimogiri, T., Kawabe, K., Nishibori, M., Okamoto, S., Hashiguchi, T. & Maeda, Y. (2009). Genotypic frequency in Asian native chicken populations and gene expression using Insulin-like Growth Factor I (IGF1) gene promoter polymorphism. Poultry Science, 46, 1-5.
  23. Moradian, H., Esmailizadeh, A., Sohrabi, S., Nasirifar, E., Askari, N., Mohammadabadi, M.R. & Baghizadeh, A. (2014). Genetic analysis of an F2 intercross between two strains of Japanese quail provided evidence for quantitative trait loci affecting carcass composition and internal organs. Molecular Biology Reports, 41, 4455-4462.
  24. Nagaraja, S.C., Aggrey, S.E., Yao, J., Zadworny, D., Fairfull, R.W. & Kühnlein, U. (2000). Trait association of a genetic marker near the IGF-I gene in egg-laying chickens. Heredity, 91, 150-156.
  25. Nestor, K.E., Bacon, W.L., Anthony, N.B. & Noble, D.O. (1996). Divergent selection for body weight and yolk precursor in Coturnix cotornix Japanica. 10. Response to selection over thirty generation. Poultry Science, 75, 303-310.
  26. Nie, Q., Lei, M., Ouyang, J., Zeng, H., Yang, G. & Zhang, X. (2004). Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography. Genetics Selection Evolution, 37, 339-360.
  27. Nikzad, S., Ghorbani, A., Salamatdoust, R. & Maheri, N. (2012). IGF-I gene (exon 1) polymorphism in four colorful strain of the female Japanese quail. Annals of Biological Research, 3(6), 2979-2982.
  28. Sohrabi, S., Esmailizadeh, A., Baghizadeh, A., Moradian, H., Mohammadabadi, M.R., Askari, N. & Nasirifar, E. (2012). Quantitative trait loci underlying hatching weight and growth traits in an F2 intercross between two strains of Japanese quail. Animal Production Science, 52(11), 1012-1018.
  29. Zhou, M., Ma, Z. & Sly, W.S. (1995). Cloning and expression of the cDNA of chicken cation-independent mannose-6-phosphate receptor. In: Proceedings of the National Academy of Sciences of the United States of America, 92, 9762-9766.
  30. Zhou, H., Mitchell, A.D., McMurtry, J.P., Ashwell, C.M. & Lamont, S.J. (2005). Insulin-like growth factor-I gene polymorphism associations with growth, body composition, skeleton integrity, and metabolic traits in chickens. Poultry Science, 84, 212-219.