شناسایی یک چندشکلی جدید در راه‌انداز ژن عامل رشد شبه انسولین-1 (igf1) در بلدرچین ژاپنی به روش PCR-SSCP

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی سابق دکتری، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 دانشیار، دانشکدۀ کشاورزی، دانشگاه صنعتی اصفهان

4 استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران

5 استاد، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان

چکیده

ژن عامل رشد شبه انسولین-1 (igf1) یک ژن کاندیدا برای بررسی صفات مرتبط با رشد، وزن بدن و رشد عضله در گونه­های مختلف است. به‌منظور بررسی ارتباط این ژن با صفات رشد در بلدرچین، هشت جفت بلدرچین سفید و وحشی به‌عنوان جمعیت صفر برای تولید افراد نسل اول (f1) با یکدیگر تلاقی داده شدند. از تلاقی بین 34 قطعه بلدرچین در نسل F1، 422 قطعه بلدرچین در نسل دوّم f2)) ایجاد شد. رکوردهای مربوط به وزن بدن در سنین مختلف در نسل دوّم ثبت و ارتباط بین چندشکلی بخشی از جایگاه راه‌انداز (پروموتور) ژن IGF1 و این رکوردها بررسی شد. با استفاده از روش PCR-SSCP یک چندشکلی تک نوکلئوتیدیG
15 → '> a  در ناحیة موردنظر ژن IGF1 در 472 قطعه بلدرچین در سه نسل تشخیص داده شد. فراوانی­ ژنوتیپ­های AA، AG و GG به ترتیب 05/0، 62/0 و 33/0 و فراوانی همردیف­ (آلل­)های ژنی A و G به ترتیب 36/0 و 64/0 محاسبه شد. وزن بدن در سن 1 تا 5 هفتگی و زمان کشتار و همچنین میانگین افزایش وزن روزانه از هفتۀ اول تا چهارم پرورش در ژنوتیپ AA، کمتر از ژنوتیپ AG و GG بود و این تفاوت ازنظر آماری معنی­دار نبود. میانگین افزایش وزن روزانة ماده­ها در ژنوتیپ AG بیشتر از نرها بود (001/0P<). نتایج ارائه‌شده در این نوشتار در مقایسه با گزارش‌های دیگر در مرغ، نشان‌دهندۀ یک چندشکلی جدید در ناحیۀ راه‌انداز ژن IGF1 در بلدرچین بود، اما ارتباط آن با صفات مرتبط با وزن بدن و نرخ رشد ازلحاظ آماری معنی­دار نبود.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of novel SNP in promoter of Insulin-Like Growth Factor-I (IGF1) gene in Japanese quail by PCR-SSCP assay

نویسندگان [English]

  • Homa Aarabi 1
  • Mohammad Moradi Shahrbabak 2
  • Abbas Pakdel 3
  • Hossein Moradi Shahrbabak 4
  • Ali Esmailizadeh koshkoiyeh 5
1 Former Ph.D. Student, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Faculty of Agriculture, University of Isfahan, Iran
4 Assistant Professor, Department of Animal Science, Agriculture & Natural Resources, University of Tehran, Karaj, Iran
5 Professor, Faculty of agriculture, Shahid Bahonar University of Kerman, Iran
چکیده [English]

Insulin-like growth factor-1 gene (IGF1) is a biological candidate gene for investigation traits such as growth, body weight and muscles growth in different species. In this research eight pairs of white and wild quails were crossed reciprocally as a base population. A total of 34 quails were produced in first generation (F1) and 422 quails were generated by intercrossing the F1 population. Body weights at the time of hatching and different weeks were recorded in the second generation. The association between IGF1 promoter region polymorphism and body weight in different ages was investigated in F2 generation. Using PCR-SSCP assay and sequencing, a novel single nucleotide polymorphism (SNP) was identified in IGF1 promoter region in 472 birds from three Generations. Genotypic frequencies of AA, AG and GG genotypes were 0.05, 0.62 and 0.33, respectively for all generations. The frequency of A and G alleles were 0.36 and 0.64, respectively. The AA genotype was lower than AG and GG genotypes for body weights in ages of one to five weeks and slaughter time and the average of daily gain was also lower from week first to four but these differences weren’t significant. The average of daily gain was higher in females compared to males for AG genotype (P<0.001). Comparison between detected allele in the present study with reported allele by other research groups in chickens revealed a novel SNP in promoter of IGF1 gene in Japanese quail, but association between this polymorphism with body weight and growth rate were not significant.

کلیدواژه‌ها [English]

  • growth traits
  • Insulin-Like Growth Factor-I
  • Japanese quail
  • PCR-SSCP assay
  • sequencing

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سابقه مقاله

  • تاریخ دریافت: 08 مهر 1394
  • تاریخ بازنگری: 12 اسفند 1394
  • تاریخ پذیرش: 19 اسفند 1394

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