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اثر سطوح جیره‌ای فسفولیپیدهای فعال بر پاسخ سامانۀ ایمنی خونی در جوجه‌های نر گوشتی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

2 استادیار گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

3 دانشیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج

چکیده

این مطالعه برای ارزیابی تأثیرات استفاده از سطوح جیره‌ای منبع فسفولیپیدهای فعال در جیرۀ غذایی بر پاسخ سامانۀ ایمنی جوجه‌های گوشتی، با 180 قطعه جوجۀ نر گوشتی سویۀ راس 308 اجرا شد. جیره‌های آزمایشی شامل سطوح 0، 2/0، 4/0، 6/0، 8/0، 1، 2/1، 4/1، 6/1 درصد منبع فسفولیپیدهای فعال به نحوی تهیه شدند که انرژی قابل سوخت‌وساز، پروتئین و سایر مواد مغذی در آن‌ها یکسان بود. خون‌گیری در 16 روزگی برای سنجش پادتن علیه ویروس نیوکاسل، در 41 روزگی برای سنجش تفریقی گلبول‌های سفید و در 36 روزگی و 45 روزگی برای تست تیتر پادتن علیه پادگن گلبول قرمز گوسفند انجام گرفت. نتایج نشان داد که سطح جیره‌ای منبع فسفولیپیدهای فعال بر اندام‌های لنفاوی، تعداد هتروفیل، لنفوسیت، مونوسیت، بازوفیل، نسبت هتروفیل به لنفوسیت، تیتر آنتی‌بادی‌های G و M به علاوه تیتر آنتی‌بادی ضد نیوکاسل اثر معناداری نداشت. در نتیجه، به نظر می‌رسد که سطوح استفاده‌شدۀ منبع فسفولیپیدهای فعال، اثری بر فراسنجه‌های ایمنی ارزیابی‌شده در این آزمایش نداشتند.

کلیدواژه‌ها


عنوان مقاله [English]

The effects of feeding different dietary levels of active phospholipids on humeral immune system response in male broilers

نویسندگان [English]

  • Haniyeh Sadat Banikamal 1
  • Mahdi Zhandi 2
  • Malak Shakeri 2
  • Hossin Moravej 3
1 M. Sc. Student, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2 Assistant Professor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 Associate Profrssor, Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

This study was carried out to investigate the effects of dietary levels of an active phospholipids source on immune system response in broilers using 180 male Ross 308 broilers chicks. Experimental diets containing 0, 0.2, 0.4, 0.6, 0.8, 1, 1.2, 1.4, 1.6 % of active phospholipids were prepared in a way that metabolizable energy, protein and other nutrients was the same in them. Blood samples were collected at days 16 for Newcastle test, day 41 for differential measurement of white blood cells and at days 36 and 45 for SRBC test. The obtained results showed that different levels of active phospholipids source had no significant effect on lymphoid organs, number of heterophil, lymphocyte, monocyte, basophil, heterophil to lymphocyte ratio, IgG and IgM titer as well as anti-Newcastle antibody titer in broilers. In conclusion, it seems that levels of used active phospholipids source had no effect on the evaluated immune parameters in this experiment.

کلیدواژه‌ها [English]

  • Antibody
  • broiler
  • Immunity
  • newcastle
  • Phospholipid
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