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شناسایی جایگاه‌های صفات کمّی مؤثر بر صفات لاشه و اندام‌های داخلی کروموزوم شمارۀ دو بلدرچین ژاپنی

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، گروه علوم دامی، تهران

2 دانشیار، گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

3 کارشناس ارشد گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

4 دانشجوی دکتری گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه شهید باهنر کرمان، کرمان

چکیده

برای مکان‌یابی جایگاه‌های صفات کمّی (QTL) مؤثر بر صفات لاشه و اندام‌های داخلی کروموزوم شمارۀ دو بلدرچین ژاپنی، با استفاده از تلاقی متقابل دو سویۀ پرندۀ سفید (تخم‌گذار) و وحشی (گوشتی)، پرندگان نسل F1 ایجاد شدند. 422 پرندۀ نسل F2 حاصل از تلاقی تصادفی پرندگان نسل F1 در پایان دورۀ 35 روزۀ پرورش کشتار شدند و رکوردبرداری فنوتیپی صفات مربوط به لاشه انجام گرفت و نمونه‌های خون آن‌ها برای تعیین ژنوتیپ چهار نشانگر ریزماهواره واقع روی کروموزوم دو جمع‌آوری شد. داده‌های مربوط به تعیین ژنوتیپ و رکوردبرداری فنوتیپی با روش نقشه‌یابی درون فاصله‌ای مبتنی بر تابعیت و با استفاده از 3 مدل آماری متفاوت مورد تجزیه و تحلیل QTL قرار گرفتند و در مجموع 15 جایگاه معنا‌دار مربوط به این صفات شناسایی شدند. در مدل اول تمامی صفات با اثر غلبه معنا‌دار شدند. در مدل دوم تمامی صفات نزدیک نشانگر GUJ0084 قرار داشتند و اغلب صفات QTLهای معنا‌داری با اثرات غلبه و ایمپرینتینگ داشتند. در مدل سوم نیز بیشتر صفات در جنس ماده و با اثر غلبه معنا‌دار شدند. نتایج این پژوهش نقش توارث غیر مندلی (ایمپرینتینگ ژنومی) و اثرات پلیوتروپی و تک‌‌‌‌‌‌‌‌‌ژنی را روی بعضی صفات نشان می‌دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Identification of quantitative trait loci affecting carcass traits and internal organs on chromosome two in Japanese quail

نویسندگان [English]

  • Ehsan Nasirifar 1
  • Ali K. Esmailizadeh 2
  • Hasan Moradian 3
  • Saeed Sohrabi 4
1 Ph. D. Student, Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Associate Professor, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
3 Former M. Sc. Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
4 Ph. D. Student, Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده [English]

The aim of present research was to locate genomic loci (QTL) on chromosome 2. Itis associated with the carcass traits in Japanese quail. The F1 population was created by using reciprocal crosses between two strains of white birds (layer) and wild (broiler) birds. The F2 birds (422 birds) were derived by random mating of the F1 birds. All of the F2 birds were slaughtered at 35 days of age and recorded for carcass traits. Blood samples were collected at slaughtering time to genotype for four microsatellite markers on chromosome 2. Genotypic and phenotypic data were analyzed for QTL mapping with interval mapping method based on regression applying three different genetic models. A total number of 15 loci was found to be significantly associated with carcass traits. In the first model all traits with dominance effect were significant. The second model often had significant QTL with dominance and imprinting effects and all the traits were closest to the GUJ0084 marker. When it comes to the third model, most of the traits were significant in the female with dominance effect. Results of this study showed the role of Non-Mendelian inheritance (genomic imprinting) and pleiotropy as well as single gene effects in some traits.

کلیدواژه‌ها [English]

  • imprinting
  • Japanese quail
  • microsatellite markers
  • QTL mapping
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