نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه پاتوبیولوژی، بخش انگل شناسی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه،ایران
2 مرکز تحقیقات آرتمیای کشور، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران.
چکیده
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Ichthyophthirius multifiliis is a ciliated parasite of freshwater fish with global distribution, which causes economically important infection in cold-water fish. The aim of this research was to conduct an experimental study on the effects of hydro-alcoholic extracts of thyme (Thymus vulgaris) and lavender (Lavandula angustifolia) plants on theront Ichthyophthirius multifiliis isolated from rainbow trout (Oncorhynchus mykiss). Ingredients of hydro-alcoholic extracts of thyme and lavender were analyzed using gas chromatography-mass spectrometry (GC-MS). Hydro-alcoholic extracts of thyme (0.05 mg/µl) and lavender (0.05 mg/µl) were prepared using methanol and Soxhlet apparatus. In this study, concentrations of 40, 20, 10, 5, 2.5, and 1.25 mg/L were prepared from hydro-alcoholic of thyme and lavender plants with three replicates for each concentration. The duration of fatality of the extracts was evaluated at 23°C for 4 hours. GC-MS analyses elucidated that there were 36 (25.75%thymol) and 23 (17.04% lavender) components of hydro-alcoholic extracts of thyme and lavender, respectively. Fatality effect of hydro-alcoholic extracts of thyme and lavender had significant association with different concentrations. The highest effect of the extracts was at 40 mg/L of hydro-alcoholic extract of thyme (82.77 ± 1.71) and lavender (62.77 ± 1.79). The numbers of theronts were decreased with increasing concentration and exposure time in different concentrations of aqueous alcoholic hydro-alcoholic extracts of T. vulgaris and L. angustifolia. The fatality effect of hydro-alcoholic extract of T. vulgaris was higher than L. angustifolia. This study uncovered that different concentrations of hydro-alcoholic extracts of garden thyme and lavender destroyed I. multifiliis theronts under laboratory conditions.
کلیدواژه‌ها [English]
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