پویش ژنوم برخی صفات تولیدمثلی گاو هلشتاین ایران

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، دانشگاه تهران، تهران، ایران

چکیده

هدف از انجام این تحقیق شناسایی نشانگرهای تک نوکلئوتیدی موثر بر برخی صفات تولیدمثلی در گاوهای شیری هلشتاین ابران است. برای این منظور از نمونه مو 150 راس گاو که بین سال­های 1390-1392 در یکی از گاوداری های شرکت فردوس پارس متولد شده بودند، جهت تعیین ژنوتیپ با تراشه حاوی SNP 30108 استفاده شد. بعد از انجام مراحل کنترل کیفیت  داده­ها با استفاده از روش تجزیه واریانس حداقل مربعات با استفاده از رویه GLM، آنالیز GWAS انجام گردید. نتایج آنالیز نشان داد 2 تغییر تک نوکلئوتیدی با صفت فاصله زایش تا  آبستنی و 12 تغییر تک نوکلئوتیدی با صفت فاصله بین اولین و آخرین تلقیح ارتباط معنی­داری (pValue<0.04) داشتند، در صفت روزهای باز  11 و برای صفت تعداد تلقیح به ازای آبستنی نیز 5 تغییر تک نوکلئوتیدی معنی­دار (pValue<0.04) وجود داشت. بررسی ها بعد از مکان یابی ژنها  تعدادیQTL و ژن­های مختلفی را  شناسایی کرد که بر صفات مورد بررسی اثرگذار یا کنترل کننده بودند. از جمله این ژن­ها می­توان به ژن NUF2  در کروموزوم 3 که بر تکامل سلول تخم، ژن ANAPC1  در کروموزوم 11 که بر جنبانی اسپرم، ژن PITX2  در کروموزوم 6 که بر تولید شیر، ژن ELOVL6 در کروموزوم 6 که بر افزایش وزن بدن، موثر است اشاره نمود. به طور کلی می­توان نتیجه گرفت که کروموزوم 3، 6 و 11 دارای تغییرات تک نوکلئوتیدی هستند که در ارتباط قوی تری با صفات تولیدمثلی بوده و در حاشیه همین تغییرات تک نوکلئوتیدی، QTL و ژن هایی یافت شد که مرتبط با صفات تولید مثلی بوده اند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Genome-Wide Association Studies for Selected Reproductive traits in Iranian Holstein cattle

نویسندگان [English]

  • Mostafa Sdeghi
  • Narges Maddahi
  • Ardeshir Nejati-Javaremi
  • Seyed Reza Miraei-Ashtiani
  • Ali Jalil Sarghale
Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran , University of Tehran, Tehran, Iran
چکیده [English]

The aim of this study was to identify Single Nucleotide Polymorphism (SNP) associated with selected reproductive traits in Holstein dairy cattle. The interval between calving and the first insemination, days open, the interval between the first and last insemination, and the number of inseminations per pregnancy were analyzed. For this purpose, the hair samples of 150 cattle, born between 2012-2014 in one of the cattle farms of Ferdous Pars company, were used for genotyping based on 30108 SNPs chip. The data were analyzed using the least square variance analysis method using the GLM. GWAS analysis was performed after controlling the quality of the data. The results identified that 2 and 12 SNPs had a significant correlation with calving to insemination interval and interval between first to last insemination traits(p<0.04), respectively. In addition, 11 SNPs and 5 SNPs showed significant correlation for days open and the number of insemination per conception traits (p<0.04), respectively. In the Post-GWAS stage, some Quantitative Trait Loci (QTL) and various genes were identified that affected or controlled the trait. Out of the related NUF2 gene on chromosome 3 affects the development of the oocyte, ANAPC1 gene on chromosome 11, which affects sperm motility, PITX2 gene in chromosome 6, affects milk production and ELOVL6 gene on chromosome 6 affects body weight. In general, it can be concluded that chromosomes 3, 6 and 11 have SNPs that are more strongly related to reproductive traits.

کلیدواژه‌ها [English]

  • Days open
  • Day to first insemination
  • interval from first to last insemination

Extended Abstract

Introduction

The main goal in dairy industry is to achieve maximum profitability and the goal of animal breeding is to increase the economic efficiency of the system. Today, with vast advances in the field of molecular methods and the identification of a large number of genetic markers, including Single Nucleotide Polymorphic (SNP) markers, livestock breeders can investigate and find QTLs affecting these traits with higher accuracy. Genome-Wide Association Studies (GWAS) as a procedure might be used to find loci correlated with reproductive traits in Iranian Holstein cows.

Materials and methods

For this purpose, the hair samples of 150 cattle, born between 2012-2014 in one of the cattle farms of Ferdous Pars company, were used for genotyping based on 30.108 SNPs chip. The data were analyzed using the least square variance analysis method using the GLM. GWAS analysis was performed after controlling the quality of the data.

 

Results and Discussion

The results showed that 2 SNPs associated with the Days to first services and 12 SNPs associated with Interval from First to Last Insemination (p<0.04). 11 SNPs in the Days Open and 5 SNPs for the Number of Services per Conception showed significant correlation (p<0.04) with these traits. In the Post-GWAS stage, some Quantitative Trait Loci and various genes were identified that affected or controlled the traits. QTLs, were observed near the SNPs related to IFL, DO and NSPC traits, they are related to body weight traits. So the heifer must have reached optimal weight at insemination time. In BTA20, near the SNP association on day to the first insemination, 1 QTL was associated with the stillbirth trait and 1 QTL with high correlation Rump angle trait was observed. Stillbirth and suitable Rump angle can be effective in returning the uterus to normal conditions and improving reproductive traits. Several QTLs affected on mamma structure traits (Udder width, Udder attachment, Udder height), milk production (Milk yield, Milk fat percentage) were observed in BTA20 and BTA27 in the vicinity of SNPs related to DFS traits. So in the near the SNPs identified, there are most QTLs that are correlated with milk production, reproduction, physical index and health traits. Out of the related genes, RHO gene on chromosome 28 is effected on ovulation, the length of the reproductive cycle and fertility. CAMK2D gene in chromosome 6 was related to the number of insemination per conception and fertility techniques. In BTA21, GABRB3 gene was related to the beginning and end of lactation in cows, and lactation is one of the effective traits for the number of inseminations per conception. NUF2 gene on chromosome 3 that affects the development of the oocyte, ANAPC1 gene on chromosome 11, which affects sperm motility, PITX2 gene in chromosome 6, affected on milk production, ELOVL6 gene in chromosome 6 affects increases body weight, CAMK2D gene in chromosome 6 is effective artificial insemination number trait and fertility techniques.

 

Conclusion

it can be concluded that chromosomes 3, 6 and 11 have SNPs that are more strongly related to reproductive traits.

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علوم دامی ایران
دوره 55، شماره 3
مهر 1403
صفحه 565-581

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  • تاریخ دریافت: 25 شهریور 1402
  • تاریخ بازنگری: 04 دی 1402
  • تاریخ پذیرش: 07 دی 1402

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