نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

2 استاد، گروه علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

3 استادیار، گروه علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران

چکیده

این آزمایش به‌ منظور مقایسه الگوی جذب و کارآیی منابع مختلف متیونین شامل منابع آزاد کریستاله مصنوعی (دی- ال- متیونین (DL-Met)، ال- متیونین (L-Met) و دی‌پپتید (Met-Met)) و متیونین باند ‌شده به پروتئین (PB-Met) در مرغ‌های ‌گوشتی با مصرف تک‌وعده‌ای خوراک انجام شد. تعداد 100 قطعه جوجه‎ گوشتی ماده سویه راس 308، در قالب طرح کاملاً تصادفی با پنج جیره‎ آزمایشی (پایه، DL-Met2.0، L-Met2.0، Met-Met2.0 و PB-Met2.0) به دلیل مطابقت با رژیم تک وعده­ای نیمچه­های مادر گوشتی از سن 39 تا 60 روزگی تغذیه شدند. در طی دوره آزمایش، میزان 90 گرم خوراک پلت 1 بار در روز به هر مرغ داده می‌شد که طی مدت 5/2±17 دقیقه مصرف می‌گردید. نتایج نشان دادند که مکمل ‌سازی جیره‌ با متیونین باند ‌شده به پروتئین (PB-Met2.0) موجب بهبود معنی‌دار (01/0P<) عملکرد رشد در مقایسه با مصرف آمینو اسیدهای مصنوعی شد. حداکثر غلظت متیونین پلاسمای خون، 1 ساعت پس از مصرف جیره‌‌های حاوی DL-Met2.0 و Met-Met2.0 و 2 ساعت بعد از مصرف جیره‌های حاوی PB-Met2.0 و L-Met2.0 مشاهده شد (01/0P<). بالا‌ترین میزان پروتئین لاشه و کارآیی پروتئین (01/0P<) و همچنین پایین‌ترین میزان نیتروژن فضولات (05/0P<) در تیمار حاوی PB-Met2.0 مشاهده شد. کارآیی آمینو اسید‌های ضروری خصوصاً متیونین، در مرغ‌های تغذیه ‌شده با جیره حاوی PB-Met افزایش یافت (01/0P<). در نتیجه به ‌نظر می‌رسد که در مرغ‌های ‌گوشتی با مصرف تک‌وعده‌ای خوراک،  DL-Met و Met-Met به دلیل عدم همزمانی جذب با سایر آمینو اسیدها، بهره­وری کمتری دارند. بنابراین همزمان ‌سازی جذب آمینو اسید متیونین با استفاده از منابع مناسب، موجب افرایش کارآیی آن می‌شود. در نتیجه PB-Met به‌عنوان منبع ترجیحی مکمل متیونین در جیره مرغ‌های‌ گوشتی با مصرف‌ تک‌وعده‌ای خوراک پیشنهاد می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of the absorption kinetics and utilization of crystalline-free and protein-‎bound methionine sources in broilers with single-meal feed consumption

نویسندگان [English]

  • Mona Zamani 1
  • Mojtaba Zaghari 2
  • Fatemeh Ghaziani 3

1 Ph.D. Candidate, Department of Animal Science, College of Agriculture & Natural Resources, ‎University of Tehran, Karaj, Iran

2 Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, Department of Animal Science, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

چکیده [English]

This experiment was performed to compare the absorption kinetics and utilization of different methionine (Met) sources including synthetic-free crystalline DL-Met (DL-Met), L-methionine (L-Met) and dipeptide (Met-Met) and protein-bound methionine (PB-Met) in broilers with single-meal feed consumption. A total of 100 female Ross 308 broiler chickens were fed in a completely randomized design with five experimental diets (basal, DL-Met2.0, L-Met2.0, Met-Met2.0 and PB-Met2.0) in accordance with compliance to the single-meal broiler breeder from 39 to 60 days of age. During experimental period, 90 grams of pellet feed was given per chicken once a day, which was consumed within 17±2.5 minutes. The results showed that dietary supplementation with PB-Met2.0 caused a significant improvement (P<0.01) in growth performance, compared to the consumption of synthetic amino acids. The maximum blood plasma methionine concentration was observed at 1 hour after consumption of diets containing DL-Met2.0 and Met-Met2.0 and 2 hours after consumption of diets containing PB-Met2.0 and L-Met2.0 (P<0.01). The highest carcass protein content and protein utilization efficiency (P<0.01) and also the lowest excreta nitrogen content (P<0.05) were observed in treatment containing PB-Met2.0. The efficacy of essential amino acids, especially methionine was increased in chickens fed diet containig PB-Met (P<0.01). As a result, it seems that DL-Met and Met-Met have less efficiency in broilers with single-meal feed consumption, due to the lack of absorption synchronization with other amino acids. Therefore, the absorption synchronization of methionine using appropriate sources increases its efficiency. In conclusion, PB-Met is recommended as a preferred source of methionine in diet of broilers with single-meal feed consumption.

کلیدواژه‌ها [English]

  • Amino Acid Imbalance
  • Excreta Nitrogen
  • Glutathione
  • Homocysteine
  • Protein Efficiency
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