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مطالعه جامع شیوع پاروویروس سگ‌سانان (‏CPV‏) در ایران و مقایسه فیلوژنتیکی ناحیه ژن ‏VP2‎‏ ‏سویه‌های غالب ایرانی با سایر سویه‌های شایع

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری تخصصی ژنتیک و اصلاح نژاد دام، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه ‏تهران، کرج ‏

2 استاد، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج ‏

3 دانشیار، گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج ‏

4 استادیار ویروس‌شناسی، دانشگاه علوم پزشکی البرز

چکیده

یکی از اصلی­ترین چالش­هایی که این روزها گله‌داران، صاحبان سگ­ها و یا مراکز آموزش و پرورش سگ­های کار را تهدید می­کند مربوط به بیماری­ پاروویروس (CPV) سگ­سانان است. این ویروس در طی سال‌ها از زمان شناسایی تا کنون به انواع و سویه‌های متعددی انشقاق یافته که در نتیجه تغییرات ژنتیکی و آمینو اسیدی، تابع آن بوده و موجب شده طیف وسیعی از خانواده گوشت­خواران را در سراسر جهان تهدید کند. بهترین راه مبارزه با تبعات این بیماری واکسیناسیون می‌باشد. لذا با توجه به عدم وجود مطالعات کافی از نحوه پراکنش و شیوع سویه‌های مختلف این ویروس که ممکن است از علل برجسته خطای واکسن و درگیری بالای سگ­‌های کشور با این ویروس باشد در این تحقیق با مطالعه مولکولی و مقایسه فیلوژنتیکی سویه‌های ایرانی نسبت به سایر سویه‌های شایع جهان دریافتیم که سویه غالب شایع در کشور از نوع CPV-2a بوده و با شیوع 24 درصدی و ضمن اختلاف ژنتیکی در چندین نقطه از ژن VP2 با دیگر سویه‌ها احتمالاً شاهد انتشار سویه جدیدی از این ویروس در کشور هستیم. درضمن بر اساس درخت فیلوژنی رسم شده مشخص شد که ایزوله­های ایرانی آن سویه اختلاف توپولوژیکی نسبتاً زیادی نسبت به سویه‌های منشأ واکسن‌های تجاری رایج دارند که فرض اولیه این تحقیق را حمایت می­کند.

کلیدواژه‌ها


عنوان مقاله [English]

A comprehensive study of the prevalence of canine parvovirus (CPV-2) in Iran and ‎phylogenetic comparison of dominant Iranian CPV-types with other global types ‎based on VP2 gene ‎

نویسندگان [English]

  • Reza Faraji 1
  • Ardeshir Nejati Javaremi 2
  • Mostafa Sadeghi 3
  • Reza Miraei Ashtiani 2
  • Hamid Reza Mozhgani 4
1 Ph.D. Candidate in Animal Breeding & Genetics, College of Agriculture & Natural Resource, ‎Unversity of Tehran, Karaj, Iran
2 Professor in Animal Breeding & Genetics, College of Agriculture & Natural Resource, Unversity of Tehran, Karaj, Iran
3 Associate Professor in Animal Breeding & Genetics, College of Agriculture & Natural Resource, Unversity of Tehran, Karaj, Iran
4 Assistant Professor of Virology, Alborz University of Medical Sciences, Karaj, Iran‎
چکیده [English]

Nowadays one of the major challenges facing herders, dog owners, or working dog training centers is canine parvovirus (CPV-2). The virus has split into several types over the years since it discovered and resulted to genetic and amino acid changes that threaten a wide range of carnivorous around the world. The best way to avoiding the consequences of this disease is vaccination. This study were designed, due to the lack of sufficient studies on the distribution and prevalence of different types of this virus, which may be a prominent cause of vaccine errors, also because of high involvement of Iranian dogs with CPV. Molecular study and phylogenetic comparison of Iranian types compared to other types through the world showed that the dominant type of CPV-2 existed in Iran is CPV-2a with a prevalence of 24% and genetic differences in several points of VP2 gene with other types. We are probably facing to a new version of this virus in our country. In addition, based on the drawn phylogenetic tree, it was found that Iranian isolates on the other side have a relatively high topological difference compared to the origin types and conventional vaccines strain, which supports the initial hypothesis of this study.

کلیدواژه‌ها [English]

  • canine
  • CPV-2
  • Parvovirus
  • phylogenetic
  • VP2 gene‎
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