بررسی ظرفیت بافری چند ترکیب بافری رایج در تغذیۀ نشخوارکنندگان با استفاده از روش عیارسنجی اسید و تأثیر آن‌ها بر فراسنجه‌های تولید گاز

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد تغذیۀ دام، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان

2 دانشیار گروه علوم دامی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان

3 استاد گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

چکیده

هدف آزمایش بررسی ظرفیت بافری شماری از بافرها یا قلیایی کننده‌ها و تأثیر آن‌ها بر هضم و تخمیر جیره‌های پر کنسانتره بود. از غلظت‌های 0، 5/0، 1، 5/1 و 2 گرم در 100 میلی‌لیتر انواع بافرها و قلیایی کننده‌ها شامل: بی‌کربنات سدیم، سدیمسسکویی کربنات، اکسید منیزیم، کربنات منیزیم، بنتونیت سدیم، زئولیت و بافر ترکیبی (۷۵/۰ درصد سدیم سسکویی کربنات+ ۷۵/۰درصد بی‌کربنات سدیم) استفاده شد. pH اولیۀ محلول بافری، مقدار اسید هیدروکلریک مصرف‌شده برای تغییر ناگهانی pH به 2/5 یا زیر آن و pH نهایی ثبت شد. کمترین هیدروکلریدریک اسید مصرف‌شده برای کاهش ناگهانی pH به ترتیب مربوط به محلول بافری حاوی بنتونیت سدیم، اکسید منیزیم، کربنات منیزیم و شاهد بود و بیشترین آن برای سطح 5/1 و 2 درصد سدیم سسکویی کربنات، بافر ترکیبی و سطح 5/1 و 2 درصد بی‌کربنات سدیم بود. بافر زئولیت نیز مقاومت مناسبی در برابر تغییر pH نشان داد و تفاوت معنی‌داری با بافر ترکیبی و یا بافر بی‌کربنات سدیم نداشت. تیمارهای مورد استفاده در آزمایش تولید گاز شامل: جیرۀ شاهد یا پایه بدون افزودن بافر و مقادیر 5/0 تا 2 درصد بی‌کربنات سدیم، 5/0 تا 2 درصد سدیم سسکویی کربنات، 5/0 درصد بنتونیت سدیم، 2 درصد کربنات منیزیم، 5/1، 2 و 5/2 درصد زئولیت و بافر ترکیبی بود که به جیرۀ پایه افزوده شدند. بیشترین گاز تولیدشده مربوط به بافرهای سدیم سسکویی کربنات، بی‌کربنات سدیم، بافر ترکیبی و زئولیت بود. بنابراین، از نظر ظرفیت بافری و حجم گاز تولیدشده، سدیم سسکویی کربنات بهترین جایگاه و پس‌از آن بافر ترکیبی، بی‌کربنات سدیم و زئولیت نیز بافرهای مناسبی برای استفاده در جیره‌های حاوی مواد متراکم زیاد در تغذیۀ نشخوارکنندگان ارزیابی شدند.

کلیدواژه‌ها

عنوان مقاله [English]

Investigation the buffering capacity of several conventional buffer compounds in feeding of ruminant animals by acid titration method and their effect on gas production parameters

نویسندگان [English]

  • Nasrin Mahdavirad 1
  • Morteza Chaji 2
  • Mohammad Bojarpour 2
  • Mehdi Dehghan banadaki 3
1 M.Sc. Student of Animal Nutrition, Department of Animal Science, Khuzestan Agricultural Sciences and Natural Resources University, P.O. Box 63517-73637, Mollasani, Ahvaz, Iran
2 Associate Professor, Department of Animal Science, Khuzestan Agricultural Sciences and Natural Resources University, P.O. Box 63517-73637, Mollasani, Ahvaz, Iran
3 Professor, Department of Animal Science, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
چکیده [English]

The aim of present experiment was to investigate the buffering capacity of some buffers or alkalizers, their effect on digestion and fermentation of high concentration diets. The concentration of 0, 0.5, 1, 1.5, 2 g in 100 ml from different buffers and alkalizing agents including: sodium bicarbonate, sodium sesquicarbonate, magnesium oxide, magnesium carbonate, sodium bentonite, zeolite and combined buffer (0.75% sodium bicarbonate+ 0.75% sodium sesquicarbonate) was used. The initial pH of the buffer solution, the amount of consumed hydrochloric acid for abrupt changes of pH to 5.2 or below it, and the final pH was registered. The lowest amount of the hydrochloric acid was consumed for solutions containing sodium bentonite, magnesium oxide, magnesium carbonate and control for suddently dropping their pH; and highest amount by sodium sesquicarbonate, combined buffer and sodium bicarbonate, respectively. The zeolite buffer also indicated a proper resistance to pH changes and did not have a significant difference with the combined buffer or sodium bicarbonate buffer. The treatments in gas production were included: control or basal diet without buffer, and amounts of 0.5 to 2% sodium bicarbonate, 0.5 to 2% sodium sesquicarbonate, 0.5% sodium bentonite, 2% magnesium carbonate, 1.5, 2 and 2.5% of zeolite and combined buffer, which were added to basal diet. The highest produced gas was in the sodium sesquicarbonate, sodium bicarbonate, combined buffers and zeolite. Therefore, sodium sesquicarbonate, combined buffers, sodium bicarbonate and zeolite had the best position respectively, which were evaluated as suitable buffers for using in diets containing high concentration ingredients for feeding the ruminant animals.

کلیدواژه‌ها [English]

  • Buffer capacity
  • pH
  • sodium sesquicarbonate
  • sodium bicarbonate
  • Zeolite

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  • تاریخ دریافت: 01 آذر 1396
  • تاریخ بازنگری: 06 اسفند 1396
  • تاریخ پذیرش: 15 اسفند 1396

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