بررسی کارایی تغذیه‌ای نمک‌های کلسیمی پوشش‌دار روغن ماهی در شرایط برون و درون‌تنی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، دانشکده کشاورزی، دانشگاه ارومیه

2 استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج

3 استاد، دانشکده کشاورزی، دانشگاه ارومیه

4 دانشجوی دورۀ دکتری، دانشکده کشاورزی، دانشگاه ارومیه

چکیده

در این پژوهش، ارزیابی میزان مواد مغذی و الگوی اسیدهای چرب، میزان مقاومت در برابر هیدروژنه شدن زیستی (بیوهیدروژناسیون) شکمبه‌ای، میزان آزادسازی روغن در نمک‌های کلسیمی پوشش­دار روغن ماهی در شرایط آزمایشگاهی اثر این نمک­ها در شرایط درون تنی بر میزان گوارش‌پذیری مواد مغذی و فراسنجه­های مایع شکمبه در مقایسه با روغن ماهی و نمک‌های کلسیمی بدون پوشش با استفاده از دام‌های فیستولادار مطالعه شد. پوشش­دار کردن منابع کلسیمی سبب افزایش کارایی محافظتی اسیدهای چرب غیراشباع در شکمبه شد (05/0P<). پوشش‌دار کردن مکمل‌های کلسیمی تفاوت معنی‌داری در میزان آزادسازی شکمبه‌ای مکمل‌ها ایجاد کرد ولی تفاوتی در میزان آزادسازی روغن از ترکیب مکمل‌ها در دیگر بخش‌های دستگاه گوارش و میزان آزادسازی در کل دستگاه گوارش وجود نداشت (05/0‌P<). مکمل‌های محافظت‌شده در مقایسه با روغن آزاد، سبب افزایش گوارش‌پذیری مواد مغذی مختلف شدند (05/0P<). استفاده از روغن ماهی به‌صورت محافظت نشده موجب کاهش میزان تولید استات و افزایش پروپیونات و کاهش میزان نیتروژن آمونیاکی و جمعیت پروتوزوآ شکمبه شد. بود یا نبود پوشش روی نمک‌های کلسیمی تأثیری در ارتباط با فراسنجه‌های شکمبه نداشت (05/0‌P>). پوشش‌دهی مکمل‌های کلسیمی سبب افزایش محافظت اسیدهای چرب غیراشباع در برابر هیدروژنه شدن زیستی شکمبه‌ای شده و تأثیر نامطلوبی بر شاخصه‌های مختلف تغذیه‌ای حیوانات نداشت. استفاده از میزان پوشش 10 درصد وزنی را می‌توان بهترین میزان پوشش با توجه به داده‌های مربوط به ترکیب شیمیایی، الگوی اسیدهای چرب، هیدروژنه شدن زیستی و آزادسازی در بخش‌های مختلف دستگاه گوارش دانست.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of Nutritional efficiency of encapsulated fish oil ca-salts in vitro and in vivo

نویسندگان [English]

  • Hamed Khalilvandi-Behroozyar 1
  • Mehdi Dehghan-Banadaky 2
  • Rasoul Pirmohammadi 3
  • Behzad AsadnEjad 4
1 Assistant Professor, Faculty of Agriculture, Urmia University, Urmia, Iran
2 Professor, University College of Agriculture & Natural Recouces, University of Tehran, Karaj, Iran
3 Professor, Faculty of Agriculture, Urmia University, Urmia, Iran
4 Ph.D. Candidate, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده [English]

In this research encapsulated fish oil Ca-salts were produced by using a saturated FA containing material and evaluated. Nutrient content and FA profiles of produced supplements were determined and ruminal biohydrogenation and oil releasing were examined in vitro. Additionally, in a complementary in vivo experiment, effects of dietary inclusion of fish oil, ca-salts and encapsulated ca-salts on nutrient digestibility and ruminal paramerters were evaluated using three rumen fistulated Holstein cows. Encapsulation of Ca-Salts increased protection efficiency of PUFA against ruminal biohydrogenation. Encapsulated ca-salts with 10 % of wall material (weight basis) had lower biohydrogenation afer 48-h in vitro incubation (P˂ 0.05). Encapsulation of ca-salts decreased rumen oil release but oil release in other simulated media or total tract oil release were not affected by encapsulation (P˃ 0.05). Protected supplements increased nutrient digestibility compared with fish oil (P˂ 0.05). Non-encapsulated fish oil decreased acetate and rumen N-NH3 concentration as well as protozoa `population, but increased propionic acid concentration (P˂0.05). Encapsulation of Ca-Salt did not change rumen parameters (P˃ 0.05). According to the results, it can be concluded that encapsulation of ca-salts increased protection of PUFA against ruminal biohydrogenation without worse effects on rumen parameters. Encapsulation with 10 % of wall material (weight basis) can be presented as the best treatment according to chemical composition, fatty acid profiles, rumen biohydrogenation and oil release results.

کلیدواژه‌ها [English]

  • Encapsulation
  • Omega-3
  • PUFA
  • Ruminal protection

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  • تاریخ دریافت: 11 مهر 1396
  • تاریخ بازنگری: 21 دی 1396
  • تاریخ پذیرش: 21 دی 1396

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